A multipartite interaction between Salmonella transcription factor σ28 and its anti-sigma factor FlgM: Implications for σ28 holoenzyme destabilization through stepwise binding

Journal of Molecular Biology

Volumn 306, Issue 5, 2001, Pages 915-929

  Chadsey, Meggen S ^a   Hughes, Kelly T ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

Author keywords

Anti sigma; Holoenzyme dissociation; Salmonella typhimurium; Sigma factor; Transcription

Indexed keywords

HELIX LOOP HELIX PROTEIN; HOLOENZYME; RNA POLYMERASE; SIGMA FACTOR; TRANSCRIPTION FACTOR;

AMINO ACID SUBSTITUTION; ARTICLE; DISSOCIATION CONSTANT; DNA BINDING; ENZYME ACTIVITY; ENZYME STABILITY; FLAGELLATE; GENE MUTATION; GENETIC ANALYSIS; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN ISOLATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SALMONELLA; SALMONELLA TYPHIMURIUM;

SALMONELLA; SALMONELLA TYPHIMURIUM; TYPHIMURIUM;

EID: 0035830954     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2001.4438     Document Type: Article

References (64)

1. Factor stimulating transcription by RNA polymerase
2. 70), promoters, and the kinetics of the steps of transcription intitiation
3. 70 family: Sequence conservation and evolutionary relationships
4. Eubacterial sigma factors
5. Promoter selectivity control of RNA polymerase
6. The Anti-sigma factors
7. Anti-sigma factors
8. F, contacting multiple conserved regions of the σ factor
9. F and its antisigma factor SpoIIAB
10. 70: Localization of a region involved in the interaction with core RNA polymerase
11. Use of in vitro protein synthesis from polymerase chain reaction-generated templates to study interaction of Escherichia coli transcription factors with core RNA polymerase and for epitope mapping of monoclonal antibodies
12. 32 with a small deletion in conserved region 3 of sigma has reduced affinity for core RNA polymerase
13. E affects its ability to bind core RNA polymerase
14. PCR mutagenesis identifies a polymerase-binding sequence of sigma 54 that includes a sigma 70 homology region
15. 70 subunit
16. 70 that are involved in interaction with RNA polymerase core enzyme
17. Identification of three regions essential for interaction between a σ-like factor and core RNA polymerase
18. 32 mutant with a single amino acid change in the highly conserved region 2.2 exhibits reduced core RNA polymerase affinity
19. Core-sigma interaction: Probing the interaction of the bacteriophage T4 gene 55 promoter recognition protein with E. coli RNA polymerase core
20. 32 determine core RNA polymerase binding specificity
21. The interface of σ with core RNA polymerase is externsive, conserved and functionally specialized
22. SpoIIAA governs the release of the cell-type specific transcription factor sigma F from its anti-sigma factor SpoIIAB
23. The bacteriophage T4 Asia protein: A molecular switch for sigma 70-dependent promoters
24. A mutant Escherichia coli sigma 70 subunit of RNA polymerase with altered promoter specificity
25. Altered promoter recognition by mutant forms of the sigma 70 subunit of Escherichia coli RNA polymerase
26. 70 subunit of Escherichia coli RNA polymerase
27. A salt-promoted inhibitor of RNA polymerase isolated from T4 phage-infected E. coli
28. 70 protein
29. Transcriptional analysis of the flagellar regulon of Salmonella typhimurium
30. The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons
31. Gene fliA encodes an alternative sigma factor specific for flagellar operons in Salmonella typhimurium
32. An alternative sigma factor controls transcription of flagellar class-III operons in Escherichia coli: Gene sequence, overproduction, purification and characterization
33. Transcriptional control of flagellar genes in E. coli K-12
34. Completion of the hook-basal body of the Salmonella typhimurium flagellum is coupled to FlgM secretion and fliC transcription
35. Negative regulatory loci coupling flagellin synthesis to flagellar assembly in Salmonella typhimurium
36. Molecular characterization of flgM, a gene encoding a negative regulator of flagellin synthesis in Salmonella typhimurium
37. Sensing structural intermediates in bacterial flagellar assembly by export of a negative regulator
38. Excretion of the anti-sigma factor through a flagellar substructure couples flagellar gene expression with flagellar assembly in Salmonella typhimurium
39. Genetic and molecular analyses of the interaction between the flagellum-specific sigma and anti-sigma factors in Salmonella typhimurium
40. 28 RNA polymerase holoenzyme
41. Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM
42. Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system
43. 70 factor induced by binding to the core enzyme
44. 70
45. 70 within the transcription elongation complex
46. 70 and NusA proteins. I. Binding interactions with core RNA polymerase in solution and within the transcription complex
47. 70 subunit fragment from E. coli RNA polymerase
48. The sigma subunit conserved region 3 is part of "5′-face" of active center of Escherichia coli RNA polymerase
49. 70 is implicated in the process of abortive transcription
50. Mapping the promoter DNA sites proximal to conserved regions of sigma 70 in an Escherichia coli RNA polymerase-lacUV5 open promoter complex
51. 28
52. Role of the FliA-FlgM regulatory system on the transcriptional control of the flagellar regulon and flagellar formation in Salmonella typhimurium
53. Differential regulation of multiple overlapping promoters in flagellar class II operons in Escherichia coll
54. Structure and expression of the fliA operon of Salmonella typhimurium
55. A unified view of polymer, dumbbell, and oligonucleotide DNA nearest-neighbor thermodynamics
56. Optimal conditions for directly sequencing double-stranded PCR products with sequenase
57. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
58. Regulation of proline utilization in Salmonella typhimurium: Characterization of put::Mud(Ap, lac) operon fusions
59. Maturation of the head of bacteriophage T4. I. DNA packaging events
60. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
61. Elution of proteins from sodium dodecyl sulfate-polyacrylamide gels, removal of sodium dodecyl sulfate, and renaturation of enzymatic activity: Results with sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase, and other enzymes
62. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice