Design and evaluation of new ligands for lysozyme recovery by affinity thermoprecipitation

Chemical Engineering Science

Volumn 56, Issue 19, 2001, Pages 5681-5692

  Vaidya, A A ^a   Lele, B S ^a   Deshmukh, M V ^a   Kulkarni, M G ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

Acetamido; Affinity precipitation; Ligands; Lysozyme; N isopropylacrylamide

Indexed keywords

ENZYMES; HYDROPHILICITY; ORGANIC COMPOUNDS; POLYMERS;

LIGAND CONCENTRATION;

CHEMICAL ENGINEERING;

PRECIPITATION; SEPARATION;

EID: 0035828709     PISSN: 00092509     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0009-2509(01)00169-5     Document Type: Article

References (45)

1. Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme
2. Molecular tailoring of thermoreversible copolymer gels: Some new mechanistic insights
3. Crystallographic studies of the activity of hen egg-white lysozyme
4. Characterization of the LCST behaviour of aqueous poly(N-isopropylacrylamide) solutions by thermal and cloud point techniques
5. Separation of proteins from polyelectrolytes by ultrafiltration
6. Characterization of pH-sensitive polymeric supports for selective precipitation of proteins
7. Biotin-modified submicron latex particles for affinity precipitation of avidin
8. On the binding of chitin oligosaccharides to lysozyme
9. The dependence of lysozyme activity on pH and ionic strength
10. Affinity precipitation- An alternative to fluidized bed adsorption?
11. Fractionation of natural and model egg-white protein solutions with modified and unmodified polysulfone UF membranes
12. Interactions between thermosensitive hydrogel microspheres and proteins
13. Intrinsic viscosity- molecular weight relationships for poly(N-isopropylacrylamide) solutions
14. Affinity thermoprecipitation: Contribution of the efficiency of ligand-protein interaction and access of the ligand
15. Thermoreactive water-soluble polymers, nonionic surfactants, and hydrogels as reagents in biotechnology
16. The enzyme-substrate complex in a muramidase catalyzed reaction I. Difference spectrum of complex
17. N-lower-fatty-acyl derivatives of Chitosan as adsorbents for lysozyme and chitinase
18. Separation of binary protein mixtures by ultrafiltration
19. Nitrile or amide hydrolysis to carboxylic acids
20. Purification of lysozyme by affinity chromatography
21. Smart polymers for enhanced binding and recovery in biotechnology
22. Biospecific adsorption of lysozyme onto monoclonal antibody ligand immobilized on nonporous silica particles
23. Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors
24. Studies on the active center of trypsin: The binding of amidines and guanidines as models of the substrate side chain
25. Investigation of binding site density: Effects on the interaction between cibacron blue-dextran conjugates and lysozyme
26. Thermal stability of high molecular weight polyacrylamide aqueous solutions
27. Inhibition of lysozyme by derivatives of D-glucosamine
28. Kinetic study on thermal denaturation of hen egg-white lysozyme involving precipitation
29. Macroporous chitin affinity membranes for lysozyme separation
30. Studies on the enzymic activity of lysozyme, III. The binding of saccharides
31. The binding and cleavage by lysozyme of N-acetylglucosamine oligosaccharides
32. Batch isolation of hen egg white lysozyme with magnetic chitin
33. High temperature hydrolysis of aliphatic amides
34. Separation of proteins with polyacrylic acids
35. Temperature-responsive bioconjugates. I synthesis of temperature-responsive oligomers with reactive end groups and their coupling to biomolecules
36. Cell culture on a thermoresponsive polymeric surface
37. Chitosan as an affinity macroligand for precipitation of N-acetyl glucosamine binding proteins/enzymes
38. Enhancing ligand-protein binding in affinity thermoprecipitation: Elucidation of spacer effects
39. Binding of hyaluronan to lysozyme at various pHs and salt concentrations
40. Binding properties of glycosaminoglycans to lysozyme-effect of salt and molecular weight
41. Controlled synthesis of amphiphilic block copolymers with pendant N-acetyl-D-glucosamine residues by living cationic polymerization and their interaction with WGA lectin
42. Chitin-coated celite as an affinity adsorbent for high-performance liquid chromatography of lysozyme