Enhanced plasmid DNA transfection with lysosomotropic agents in cultured fibroblasts

International Journal of Pharmaceutics

Volumn 218, Issue 1-2, 2001, Pages 81-92

  Ciftci, Kadriye ^a,b   Levy, Robert J ^a  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b TEMPLE UNIVERSITY   (United States)

Author keywords

Cell culture; Chloroquine; Gene transfer; In vitro; Lysosomotropic agents; Plasmid DNA; PVP; Sucrose

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; BETA GALACTOSIDASE; CHLOROQUINE; DIMETHYL SULFOXIDE; FORMAZAN; LIPOFECTAMINE; PLASMID DNA; POVIDONE; SUCROSE;

ANIMAL CELL; ARTICLE; CELL DEATH; CELL LINE; CELL SPECIFICITY; CELL SURVIVAL; CELL VIABILITY; CONTROLLED STUDY; CYTOTOXICITY; DNA TRANSFECTION; DRUG BIOAVAILABILITY; FIBROBLAST CULTURE; GENE EXPRESSION; GENE TARGETING; HUMAN; HUMAN CELL; NONHUMAN; OSMOTIC PRESSURE; PRIORITY JOURNAL; PROTEIN DETERMINATION;

ANIMALS; ANTIRHEUMATIC AGENTS; BETA-GALACTOSIDASE; CELL LINE; CELL SURVIVAL; CELLS, CULTURED; CHLOROQUINE; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; FIBROBLASTS; GENE THERAPY; HUMANS; LYSOSOMES; MICROSCOPY, PHASE-CONTRAST; PHARMACEUTIC AIDS; PLASMIDS; POVIDONE; SUCROSE; TRANSFECTION;

EID: 0035821383     PISSN: 03785173     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-5173(01)00623-8     Document Type: Article

References (35)

1. Optimization of non-viral gene transfer of vascular smooth muscle cells in vitro and in vivo
2. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye binding
3. Polymer based gene delivery systems: An efficient way to introduce DNA into mammalian cells
4. A promising delivery systems for cancer gene therapy
5. High-efficiency receptor mediated delivery of small and large (48 kb) gene constructs using the endosome disruption activity of defective or chemically inactivated adenovirus particles
6. Lysosomotropic agents
7. Biochemical and functional analysis of an adenovirus based ligand complex for gene transfer
8. Structure activity study of inhibition of amphotericin B (fungizone) binding to sterols, toxicity to cells and lethality to mice by ester of sucrose
9. Lipofectamine reagent: A new higher efficiency polycationic liposome transfection reagent
10. Renal changes in the rabbit resulting from intravenous injection of hypetonic solution of sucrose
11. The effects of loading on lysosomal hydrolases
12. DNA vaccine for hepatitis-B: Evidence for immunogenicity in chimpanzees and comparison with other vaccines
13. Metabolic instability of plasmid DNA in the cytosol: A potential barrier to gene transfer
14. Delivery of plasmid DNA into mammalian cells using pH sensitive liposomes: Comparison with cationic liposomes
15. Cyclic amphipathic peptide-DNA complexes mediate high efficiency transfection of adherent mammalian cells
16. Mechanism of gene uptake and expression in adult mouse skeletal muscle
17. Influence of formulation factors on gene transfer mediated by cationic polypeptides
18. High efficiency polymer DNA transfection of chloroquine treated cells
19. Chloroquine retinopathy in Nigerian patients with heart block
20. DNA-chitosan nanospheres for gene delivery
21. The effect of cytochalasin-B on the release of lysosomal enzymes and intralysosomally stored polyvinylpyrolidone in the isolated perfused rat liver
22. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays
23. Polyvinyl derivatives as novel interactive polymers for controlled gene delivery to muscle
24. Fluid endocytosis by rat liver and spleen
25. Fluid endocytosis rat parenchymal and non-parenchymal live cells
26. Hypertonic sucrose inhibition of endocytic transport suggests multiple early endocytic compartments
27. Intracellular digestion of saturated and unsaturated phospholipid liposomes by mucosal cells. Possible mechanism of transport of liposomally entrapped macromolecules across the isolated vascularity perfused rabbit illeum
28. The effect of povidone-iodine solution applied at the conclusion of ophthalmic surgery
29. Chronic toxicity and carcinogenicity study of isomalt in rats and mice
30. Evidence for both prelysosomal and lysosomal intermediates in endocytic pathways
31. Coupling adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor mediated gene delivery and expression of transfected genes
32. Delivery Of CFTR gene to rabbit airways by gelatin DNA microspheres
33. Cardiac lysosomal derangements in mouse heart after long term exposure to non metabolizable sugars
34. Penetration of cells by herpes simplex virus doe not require a low pH-dependent endocytic pathway
35. Mechanism of oligonucleotide release from cationic liposomes