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At 40% DOPS, the system behaved irreproducibly. The p6-p3 transition was observed in some 10-15% of the experiments. On the other hand, at 50% DOPS and more, the transition was observed systematically.
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40
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Mechanical stress associated with transfer of the crystals to continuous carbon film is sufficient to induce the p6-p3 transition on lipid monolayers containing 20% DOPS. It may be that the p3 phase observed by EM in these conditions is in a region of the phase diagram where it is metastable. This might explain why better ordered p3 crystals are usually observed in negatively stained preparations, which require drying, as compared to those embedded in vitreous ice, which do not.
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