Secretory expression of human insulin in methylotrophic yeast pichia pastoris

Acta Biochimica et Biophysica Sinica

Volumn 31, Issue 5, 1999, Pages 588-589

  Wang, Yan ^a   Liang, Zhen He ^a   Zhang, You Shang ^a   Cui, Da Fu ^a   Feng, You Min ^a  

^a INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

Expression system; Pichia pastoris; Porcine insulin precursor (PIP); Recombinant human insulin

Indexed keywords

EID: 0005588933     PISSN: 05829879     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (5)

1. Cregg J M, Vedvick T S. Raschke W C. Recent advances in the expression of foreign genes in Pichia pastoris. Bio/Technology, 1993, 11: 905-910
2. Zhang Y S, Hu H M, Cai R R et al Secretory expression of a single-chain insulin precursor in yeast and its conversion into human insulin. Science in China (Series C) 1996, 39(3): 225-233
3. (Feng Y M, Zhang B Y, Zhang Y S et al.): Kluyveromyces lactis (Acta Biochimica et Biophysica Sinica), 1997, 29(2): 129-133
4. Clare J J, Romanos M A, Rayment F B et al. Production of mouse epidermal growth factor in yeast: high level secretion using Pichia pastoris strains containing multiple gene copies. Gene, 1991, 105: 205-212
5. Kjeldsen T, Brandt J, Anderson A S et al. A removable spacer peptide in an α-factor-leader/insulin precursor fusion protein improves processing and concomitant yield of the insulin precursor in Saccharomyces cerevisiae. Gene, 1996, 170: 107-112