Identification of thermostabilizing residues in a Bacillus alkaline cellulase by construction of chimeras from mesophilic and thermostable enzymes and site-directed mutagenesis

FEMS Microbiology Letters

Volumn 195, Issue 1, 2001, Pages 67-72

  Hakamada, Yoshihiro ^a   Hatada, Yuji ^a   Ozawa, Tadahiro ^a   Ozaki, Katsuya ^a   Kobayashi, Tohru ^a   Ito, Susumu ^a  

^a KAO CORPORATION   (Japan)

Author keywords

Alkaliphile; Cellulase; Ionic interaction; Mesophile; Thermostability

Indexed keywords

ASPARAGINE; ASPARTIC ACID; CELLULASE; GLUTAMIC ACID; ION; LYSINE;

ALKALINITY; AMINO ACID SEQUENCE; ARTICLE; BACILLUS; CHIMERA; ENZYME STABILITY; HYDROPHILICITY; ISOELECTRIC FOCUSING; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; THERMOSTABILITY;

ALKALIES; AMINO ACID SEQUENCE; BACILLUS; CELLULASE; CHIMERIN PROTEINS; ENZYME STABILITY; HEAT; ISOELECTRIC POINT; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ANALYSIS, DNA; SURFACE PROPERTIES;

BACILLUS SP.;

EID: 0035808971     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(00)00547-4     Document Type: Article

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