Single-particle approaches in the analysis of small 2D crystals of the mitochondrial channel VDAC

Journal of Structural Biology

Volumn 133, Issue 2-3, 2001, Pages 254-265

  Verschoor, Adriana ^a   Tivol, William F ^a   Mannella, Carmen A ^a  

^a WADSWORTH CENTER   (United States)

Author keywords

Electron microscopy; Image processing; Mitochondria; Single particle analysis; Two dimensional crystals; VDAC

Indexed keywords

MITOCHONDRIAL PROTEIN; VOLTAGE GATED CHANNEL FORMING PROTEIN;

CONFERENCE PAPER; CONTROLLED STUDY; CORRELATION ANALYSIS; CRYSTALLOGRAPHY; DATA ANALYSIS; IMAGE PROCESSING; MULTIVARIATE ANALYSIS; NONHUMAN; PARTICLE SIZE; PRIORITY JOURNAL; PROTEIN ANALYSIS; SINGLE PARTICLE ANALYSIS;

EID: 0035783160     PISSN: 10478477     EISSN: None     Source Type: Journal    
DOI: 10.1006/jsbi.2001.4355     Document Type: Conference Paper

References (28)

1. Bowie, J. U. (2000) Membrane proteins: Are we destined to repeat history? Curr. Opin. Struct. Biol. 10, 935-937.
2. Dolder, M., Zeth, K., Tittmann, P., Gross, H., Welte, W., and Wallimann, T. (1999) Crystallization of the human, mitochondrial voltage-dependent anion-selective channel in the presence of phospholipids, J. Struct. Biol. 127, 64-71.
3. Dorset, D. L., Engel, A., Häner, M., Massalski, A., and Rosenbusch, J. P. (1983) Two-dimensional crystal packing of matrix porin, a channel-forming protein in Escherichia coli outer membranes, J. Mol. Biol. 165, 701-710.
4. + conduction and selectivity, Science 280, 69-77.
5. Frank, J., Chiu, W., and Degn, L. (1988) The characterization of structural variations within a crystal field, Ultramicroscopy 26, 345-360.
6. Frank, J., Radermacher, M., Penczek, P., Zhu, J., Li, Y., Ladjadj, M., and Leith, A. (1996) SPIDER and WEB: Processing and visualization of images in 3D electron microscopy and related fields, J. Struct. Biol. 116, 190-199.
7. Frank, J., Verschoor, A., and Boublik, M. (1981) Computer averaging of electron micrographs of 40S ribosomal subunits, Science 214, 1353-1355.
8. Gabashvili, I. S., Agrawal, R. K., Spahn, C. M., Grassucci, R. A., Svergun, D. I., Frank, J., and Penczek, P. (2000) Solution structure of the E. coli 70S ribosome at 11.5 Å resolution, Cell 100, 537-549.
9. Guo, X. W. (1991) Electron Microscopic Studies of 2D Membrane Crystals of Mitochondrial Channel, VDAC, Ph.D. thesis, State Univ. of New York at Albany.
10. Guo, X. W., and Mannella, C. A. (1992) Classification of projection images of crystalline arrays of the mitochondrial, voltage-dependent anion-selective channel embedded in aurothioglucose, Biophys. J. 63, 418-427.
11. Guo, X. W., and Mannella, C. A. (1993) Conformational change in the mitochondrial channel, VDAC, detected by electron cryomicroscopy, Biophys. J. 64, 545-549.
12. Guo, X. W., Smith, P. R., Cognon, B., D'Arcangelis, D., Dolginova, E., and Mannella, C. A. (1995) Molecular design of the voltage-dependent, anion-selective channel in the mitochondrial outer membrane, J. Struct. Biol. 114, 41-59.
13. Henderson, R. (1995) The potential and limitations of neutrons, electrons and X-rays for atomic resolution microscopy of unstained biological molecules, Q. Rev. Biophys. 28, 171-193.
14. Lebart, L., Morineau, A., and Warwick, K. M. (1984) Multivariate Descriptive Statistical Analysis, Wiley, New York.
15. Mannella, C. A. (1984) Phospholipase-induced crystallization of channels in mitochondrial outer membranes, Science 224, 165-166.
16. Mannella, C. A. (1986) Mitochondrial outer membrane channel (VDAC, porin) two-dimensional crystals from Neurospora, Methods Emzymol. 125, 595-610.
17. Mannella, C. A. (1998) Conformational changes in the mitochondrial channel protein, VDAC, and their functional implications, J. Struct. Biol. 121, 207-218.
18. Mannella, C. A., Columbini, M., and Frank, J. (1983) Structural and functional evidence for multiple channel complexes in the outer membrane of Neurospora crassa mitochondria, Proc. Natl. Acad. Sci. USA 80, 2243-2247.
19. Mannella, C. A., and Frank, J. (1986) Effect of succinylation on images of negatively stained arrays of mitochondrial outer membrane channels, J. Ultrastruc. Mol. Struct. Res. 96, 31-40.
20. Mannella, C. A., and Guo, X. W. (1992) Model for a gated mito-chondrial channel based on electron microscopy of 2D crystal polymorphs, Transac. Am. Crystallogr. Assn. 28, 175-182.
21. Mannella, C. A., and Guo, X. W. (1990) Interaction between the VDAC channel and a polyanionic effector: An electron microscopic study, Biophys. J. 57, 23-31.
22. Mannella, C. A., Guo, X. W., and Cognon, B. (1989) Diameter of the mitochondrial outer membrane channel: Evidence from electron microscopy of frozen-hydrated membrane crystals, FEBS Lett. 253, 231-234.
23. Ostermeier, C., Iwata, S., and Michel, H. (1996) Cytochrome c oxidase, Curr. Opin. Struct. Biol. 6, 460-466.
24. 2 synthase-1, Nature 367, 243-249.
25. Sherman, M. B., Soejima, T., Chiu, W., and van Heel, M. (1998) Multivariate analysis of single unit cells in electron crystallography, Ultramicroscopy 74, 179-199.
26. van Heel, M., and Frank, J. (1981) Use of multivariate statistical analysis in analyzing the images of biological macromolecules, Ultramicroscopy 6, 187-194.
27. Walz, T., and Grigorieff, N. (1998) Electron crystallography of two-dimensional crystals of membrane proteins, J. Struct. Biol. 121, 142-161.
28. Weiss, M. S., and Schulz, G. E. (1992) Structure of porin at 1.8 Å resolution, J. Mol. Biol. 227, 493-509.