Fluorescence micro-imaging of living cells and biomolecules with ultra high NA objectives

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 4597, Issue , 2001, Pages 121-127

  Terakawa, Susumu ^a   Tsuboi, Takashi ^a   Sakurai, Takashi ^a   Jeromin, Andreas ^b   Wakazono, Yoshihiko ^a   Yamamoto, Seiji ^a   Abe, Katsuyuki ^c  

^a HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b MOUNT SINAI HOSPITAL   (Canada)

^c OLYMPUS CORPORATION   (Japan)

Author keywords

Exocytosis; Fluorescence resonance energy transfer; Total internal reflection fluorescence microscopy (TIRFM)

Indexed keywords

CELLS; DNA; ENERGY TRANSFER; FLUORESCENCE; IMAGE ANALYSIS; IMAGING SYSTEMS; MICROSCOPIC EXAMINATION; PROTEINS; WATER;

TOTAL INTERNAL REFLECTION FLUORESCENCE MICROSCOPY (TIRFM);

BIOMEDICAL ENGINEERING;

EID: 0035771595     PISSN: 0277786X     EISSN: None     Source Type: Journal    
DOI: 10.1117/12.446647     Document Type: Article

References (7)

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2. Y. Kawano, C. Abe, T. Kaneda, Y. Aono, K. Abe, K. Tamura, and S. Terakawa, "High numerical aperture objective lenses and optical system improved objective type total internal reflection fluorescence microscopy," Proc. of SPIE, 4098, pp. 142-151, 2000.
3. J.A. Stayer and W. Almers, "A real-time view of life within 100 nm of the plasma membrane," Nature Review, 2, pp. 268-275, 2001.
4. D. Axelrod, "Selective imaging of surface fluorescence with very highly aperture microscope objectives," J. of Biomed. Opt., 6, pp. 6-13, 2001.
5. T. Tsuboi, T. Kikuta, A. Warashina, and S. Terakawa, "Protein kinase C-dependent supply of secretory granules to the plasma membrane," Biochem. Biophys. Res. Commun., 282, pp. 621-628, 2001.
6. D. Zenisek, J.A. Steyer, and W. Almers, "Transport, capture and exocytosis of single synaptic vesicles at active zones," Nature 406, pp. 849-854, 2000.
7. T. Tsuboi, C. Zhao, S. Terakawa, and G.A. Rutter, "Simultaneous evanescent wave imaging of insulin vesicle membrane and cargo during a single exocytotic event," Current Biol., 10, pp. 1307-1310, 2000.