Simultaneous evanescent wave imaging of insulin vesicle membrane and cargo during a single exocytotic event

Current Biology

Volumn 10, Issue 20, 2000, Pages 1307-1310

  Tsuboi, Takashi ^a   Zhao, Chao ^b   Terakawa, Susumu ^a   Rutter, Guy A ^b  

^a HAMAMATSU UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0034687214     PISSN: 09609822     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-9822(00)00756-9     Document Type: Article

References (14)

1. Cell physiology. Secretion without full fusion
2. Transmitter release from synapses: Does a preassembled fusion pore initiate exocytosis?
3. Secretory-granule dynamics visualized in vivo with a phogringreen fluorescent protein chimaera
4. Transport, docking and exocytosis of single secretory granules in live chromaffin cells
5. Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy
6. Imaging constitutive exocytosis with total internal reflection fluorescence microscopy
7. Molecular cloning of phogrin, a protein-tyrosine phosphatase homologue localized to insulin secretory granule membranes
8. Glucose-induced proton uptake in secretory granules of beta-cells in monolayer culture
9. 2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy
10. 2+ concentration changes at the secretory vesicle surface with a recombinant targeted cameleon
11. Insulin targeting to the regulated secretory pathway after fusion with green fluorescent protein and firefly luciferase
12. Microtubule-dependent transport of secretory vesicles visualized in real time with a GFP-tagged secretory protein
13. Monitoring secretion in real time: Capacitance, amperometry and fluorescence compared
14. High calcium concentrations shift the mode of exocytosis to the kiss-and-run mechanism