Purification of cytomegalovirus-specific CD8 T cells from peripheral blood using HLA-peptide tetramers

British Journal of Haematology

Volumn 115, Issue 2, 2001, Pages 428-434

  Keenan, R D ^a,b   Ainsworth, J ^a   Khan, N ^a   Bruton, R ^a   Cobbold, M ^a   Assenmacher, M ^c   Milligan, D W ^b   Moss, P A H ^a  

^a UNIVERSITY DEPARTMENT OF MEDICINE   (United Kingdom)

^b UNIVERSITY HOSPITALS BIRMINGHAM NHS FOUNDATION TRUST   (United Kingdom)

^c MILTENYI BIOTEC GMBH   (Germany)

Author keywords

CD8 T cell; Cytomegalovirus; Magnetic selection; Marrow transplant; Tetramer

Indexed keywords

HLA ANTIGEN CLASS 1; PEPTIDE; TETRAMER;

ANTIGEN SPECIFICITY; ARTICLE; CELL SELECTION; CELLULAR IMMUNITY; CONTROLLED STUDY; CYTOMEGALOVIRUS; HEMATOPOIETIC STEM CELL TRANSPLANTATION; HUMAN; HUMAN CELL; IMMUNOTHERAPY; NORMAL HUMAN; PRIORITY JOURNAL; T LYMPHOCYTE; VIRUS PURIFICATION; VIRUS REACTIVATION; VIRUS REPLICATION;

ADOPTIVE TRANSFER; ANTIBODIES, VIRAL; ANTIGENS, VIRAL; CD8-POSITIVE T-LYMPHOCYTES; CYTOMEGALOVIRUS; CYTOMEGALOVIRUS INFECTIONS; HLA ANTIGENS; HUMANS; IMMUNODOMINANT EPITOPES; IMMUNOMAGNETIC SEPARATION; INTERFERON TYPE II; PEPTIDE FRAGMENTS;

EID: 0035725106     PISSN: 00071048     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2141.2001.03106.x     Document Type: Article

References (14)

1. Phenotypic analysis of antigen-specific T lymphocytes
2. Initiation of signal transduction through the T cell receptor requires the multivalent engagement of peptide/MHC ligand
3. Increased transplant-related morbidity and mortality in CMV-antibody positive patients despite highly effective prevention of CMV disease after allogeneic T-cell-depleted stem cell transplantation
4. Early reactivation of cytomegalovirus and high risk of interstitial pneumonitis following T-depleted BMT for adults with haematological malignancies
5. Cytomegalovirus seropositivity adversely influences outcome after, T-depleted unrelated donor transplant in patients with chronic myeloid leukaemia: The case for tailored graft-versus-host disease prophylaxis
6. Reverse Elispot assay for the clonal analysis of cytokine production
7. Functional heterogeneity and high frequencies of cytomegalovirus-specific CD8 (+) T lymphocytes in healthy antibody positive donors
8. Cytomegalovirus disease in adult marrow transplant recipients receiving gangcyclovir prophylaxis: A retrospective study
9. CMV antigenemia following bone marrow transplantation: Risk factors and outcomes
10. Reconstitution of CD8 T cells is essential for the prevention of multiple-organ cytomegalovirus histopathology after bone marrow transplantation
11. High gradient magnetic cell sorting
12. Reconstitution of cellular immunity against cytomegalovirus in recipients of allogeneic bone marrow by transfer of T-cell clones from the donor
13. The human cytolytic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein /pp. 65: Frequency, specificityand T-cell receptor usage of /pp. 65-Specific CTL
14. Resolution of earlt cytomegalovirus (CMV) infection after leukocyte transfusion therapy from a CMV antibody positive donor