Copurification of the Lac repressor with polyhistidine-tagged proteins in immobilized metal affinity chromatography

Protein Expression and Purification

Volumn 21, Issue 2, 2001, Pages 352-360

  Owens, Róisín M ^a   Grant, Andrew ^a   Davies, Nicholas ^a   O'Connor, C David ^a  

^a UNIVERSITY OF SOUTHAMPTON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

EID: 0035718504     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1384     Document Type: Article

References (20)

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4. Surface topography of histidine residues: A facile probe by immobilized metal ion affinity chromatography
5. Secretion in yeast: Purification and in vitro translocation of chemical amounts of prepro-alpha-factor
6. One-step purification of recombinant human papillomavirus type 16 E7 oncoprotein and its binding to the retinoblastoma gene product
7. 2+-carboxymethylaspartate-agarose Superflow, as demonstrated by one-step purification of lactate dehydrogenase from chicken breast muscle
8. BipA: A tyrosine-phosphorylated GTPase that mediates interactions between enteropathogenic Escherichia coli (EPEC) and epithelial cells
9. Plasmid transformation of Escherichia coli and other bacteria
10. Defining protease specificity with proteomics: A protease with a dibasic amino acid recognition motif is regulated by a two-component signal transduction system in Salmonella
11. In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis
12. Rapid identifcation of proteins by peptide-mass fingerprinting
13. Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases
14. Error-tolerant identification of peptides in sequence databases by peptide sequence tags
15. Protein folding and association: Insights from the interfacial and thermodynamic properties of hydrocarbons
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17. Lac repressor and lac operator
18. Crystal structure of the lactose operon repressor and its complexes with DNA and inducer
19. Purification of Lac repressor protein using polymer displacement and immobilization of the protein