Purification of Lac repressor protein using polymer displacement and immobilization of the protein

Bioseparation

Volumn 8, Issue 6, 1999, Pages 307-316

  Kumar, A ^a   Galaev, I Yu ^a   Mattiasson, B ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

IMAC; Immobilization; IPTG inducer; Lac operator; Lac repressor; Phosphocellulose; Polymer displacement

Indexed keywords

CHROMATOGRAPHY; ELUTION; ION EXCHANGE; LAC REPRESSOR PROTEIN; PLASMID DNA; PLASMID; POLYMER; PROTEIN CONFORMATION; PROTEIN DNA BINDING; PROTEIN IMMOBILIZATION; PROTEIN PURIFICATION;

ABSORPTION SPECTROSCOPY; CELLULOSE DERIVATIVES; CHEMICAL REACTORS; CHROMATOGRAPHY; DNA; ESCHERICHIA COLI; ION EXCHANGERS; PURIFICATION; SUGARS;

CATION EXCHANGE COLUMNS; LAC REPRESSOR PROTEIN; PHOSPHOCELLULOSE; POLYETHYLENEIMINE; POLYMER DISPLACEMENT;

PROTEINS;

BACTERIAL PROTEINS; CHROMATOGRAPHY, LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; POLYMERS; REPRESSOR PROTEINS;

ESCHERICHIA COLI;

EID: 0033383946     PISSN: 0923179X     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1008101711218     Document Type: Article

References (31)

1. Barker A, Fickert R, Oehler S & Muller-Hill B (1998) Operator search by mutant lac repressors. J. Mol. Biol. 278: 549-558.
2. Benson Chanda V (ed.) (1994) Current Protocols in Molecular Biology, Vol 1, John Wiley & Sons, New York.
3. Beyreuther K, Adler K, Fanning E, Murray C, Klemm A & Geisler N (1975) Amino-acid sequence of lac repressor from Escherichia coli. Isolation, sequence analysis and sequence assembly of tryptic peptides and cyanogen-bromide fragments. Fur. J. Biochem. 59: 491-509.
4. Bourgeois S & Riggs AD (1970) The lac repressor-operator interaction IV. Assay and purification of operator DNA. Biochem. Biophys. Res. Commun. 38: 348-354.
5. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal. Biochem. 72: 248-254.
6. Butler AP, Rezvin A & von Hippel PH (1977) Molecular parameters characterising the interaction of Escherichia coli lac repressor with non-operator DNA and inducer. Biochemistry 16: 4757-4768.
7. Eley J (1969) Purification of chicken pancreas nuclease by substrate elution from phosphorylated cellulose. Biochemistry 8: 1502-1506.
8. Gadam SD, Jayaraman G & Cramer SM (1993) Characterization of non-linear adsorption properties of dextran-based polyelectrolyte displacers in ion-exchange systems. J. Chromatogr. 630: 37-52.
9. Galaev I Yu, Arvidsson P & Mattiasson B (1995) Polymer displacement in dye affinity chromatography. J. Chromatogr. A 710: 259-266.
10. Gerstner JA & Cramer SM (1992) Cation-exchange displacement chromatography of proteins with protamine displacers: effect of induced salt gradients. Biotechnol. Progr. 8: 540-545.
11. Ghose S & Mattiasson B (1991) Evaluation of displacement chromatography for the recovery of lactate dehydrogenase from beef heart under scale-up conditions. J. Chromatogr. 547: 145-153.
12. Ghose S & Mattiasson B (1993) Protein adsorption to hydrophobic zeolite Y: salt effects and application to protein fractionation. Biotechnol. Appl. Biochem. 18: 311-320.
13. Gilbert W & Muller-Hill B (1966) Isolation of lac repressor. Proc. Nat. Acad. Sci. USA 56: 1891-1898.
14. Hermanson GT, Krishna Mallia A & Smith PK (eds.) (1992) Immobilized Affinity Ligand Techniques. Academic Press, Inc., San Diego, CA.
15. Jayaraman G, Gadam SD & Cramer SM (1993) Ion-exchange displacement chromatography of proteins. Dextran-based polyelectrolytes as high affinity displacers. J. Chromatogr. 630: 53-68.
16. Klint D, Arvidsson P, Blum Z & Eriksson H (1994) Purification of proteins by the use of hydrophobic zeolite Y Protein Expres. Purific. 5: 569-576.
17. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.
18. Lin SY & Riggs AD (1972) Lac operator analogues: bromodeoxyuridine substitution in the lac operator affects the rate of dissociation of the lac repressor. Proc. Natl. Acad. Sci. USA 69: 2574-2576.
19. Lundeberg J, Wahlberg J & Uhlen M (1990) Affinity purification of specific DNA fragments using a lac repressor fusion protein. Genet. Anal. Techn. Appl. 7: 47-52.
20. Matsuura N, Ohshima Y & Horiuchi T (1972) Secondary structure of the lac repressor. Biochem. Biophys. Res. Commun. 47: 1438-1443.
21. Muller-Hill B (1975) Lac repressor and lac operator. Prog. Biophys. Mol. Biol. 30: 227-252.
22. Muller-Hill B, Beyreuther K & Gilbert W (1971) Lac repressor from Escherichia coli. In: Grossman L & Moldave K (eds.) Methods in Enzymology, Vol. 21D (pp. 483-487) Academic Press, New York.
23. Ohshima Y, Matsuura M & Horiuchi T (1972) Conformational change of the lac repressor induced with the inducer. Biochem. Biophys. Res. Commun. 47: 1444-1450.
24. Porath J & Olin B (1983) Immobilized metal ion affinity adsorption and immobilized metal ion affinity chromatography of biomaterials. Serum protein affinities for gel-immobilized iron and nickel ions. Biochemistry 22: 1621-1630.
25. Riggs AD & Bourgeois S (1968) On the assay, isolation and characterization of the lac repressor. J. Mol. Biol. 34: 361-364.
26. Riggs AD, Suzuki H & Bourgeois S (1970) Lac repressor-operator interaction I. Equilibrium studies. J. Mol. Biol. 48: 67-83.
27. Rosenberg JM, Khallai OB, Kopka ML, Dickerson RE & Riggs AD (1977) Lac repressor purification without inactivation of DNA binding activity. Nucleic Acids Res. 4: 567-572.
28. Ruther U & Muller-Hill B (1983) Easy identification of cDNA clones. EMBO J. 2: 1791-1794.
29. Sambrook J, Fritsch EF & Maniatis T (1989) Molecular Cloning; Laboratory Manual. Cold Spring Harbor, NY.
30. Subramanian G, Phillips MW & Cramer SM (1988) Displacement chromatography of biomolecules. J. Chromatogr. 439: 341-351.
31. Ward JM, Wallace LJ, Cowan D, Shadbolt P & Levison PR (1991) Phosphocellulose as a tool for rapid purification of DNA-modifying enzymes. Anal. Chim. Acta 249: 195-200.