Expression, purification, and characterization of the active immunoglobulin-like domain of human granulocyte-colony-stimulating factor receptor in Escherichia coli

Protein Expression and Purification

Volumn 21, Issue 2, 2001, Pages 317-322

  Ishibashi, Matsujiro ^a   Tokunaga, Hiroko ^a   Arakawa, Tsutomu ^b   Tokunaga, Masao ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

^b ALLIANCE PROTEIN LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ESCHERICHIA COLI;

EID: 0035717689     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1381     Document Type: Article

References (13)

1. Expression cloning of a receptor for murine granulocyte colony-stimulating factor
2. Three different mRNAs encoding human granulocyte colony-stimulating factor receptor
3. Atomic structure of the GCSF-receptor complex showing a new cytokine-receptor recognition scheme
4. Dimerization of the extracellular domain of granulocyte-colony stimulating factor receptor by ligand binding: A monovalent ligand induces 2:2 complexes
5. Dimerization of granulocyte-colony stimulating factor receptor: The Ig plus CRH construct of granulocyte-colony stimulating factor receptor forms a 2:2 complex with a ligand
6. Coexpression of G-CSF with an unglycosylated G-CSF receptor mutant results in secretion of a stable complex
7. Evidence for the ligand-induced conversion from a dimer to a tetramer of the granulocyte colony-stimulating factor receptor
8. Requirement for the immunoglobulin-like domain of granulocyte colony-stimulating factor receptor in formation of a 2:1 receptor-ligand complex
9. Expression and purification of cytokine receptor homology domain of human granulocyte-colony stimulating factor receptor in Escherichia coli
10. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
11. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa
12. Protein measurement with the folin phenol reagent
13. Characterization of deletion mutations in the carboxy-terminal peptide-binding domain of the Kar2 protein in Saccharomyces cerevisiae