Coexpression of G-CSF with an unglycosylated G-CSF receptor mutant results in secretion of a stable complex

Protein Expression and Purification

Volumn 14, Issue 1, 1998, Pages 45-53

  Horan, Thomas P ^a   Simonet, Lizette ^a   Jacobsen, Rick ^a   Mann, Mike ^a   Haniu, Mitsuru ^a   Wen, Jie ^a   Arakawa, Tsutomu ^a   Kuwamoto, Moeka ^a   Martin, Frank ^a  

^a AMGEN INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CDNA LIBRARY; CELL LYSATE; CHO CELL; CRYSTALLIZATION; CYTOKINE RECEPTOR; GENE MUTATION; GLYCOSYLATION; GRANULOCYTE COLONY STIMULATING FACTOR; IMMUNOGLOBULIN RECEPTOR; LIGAND BINDING; MESSENGER RNA; MUTANT; PROTEIN EXPRESSION; PROTEIN STABILITY; STOICHIOMETRY; WESTERN BLOTTING;

CRICETINAE; CRICETULUS GRISEUS;

EID: 0032190603     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.1998.0942     Document Type: Article

References (27)

1. Nicola N. A. Hematopoietic cell growth factors and their receptors. Annu. Rev. Biochem. 58:1989;45-77.
2. Metcalf D., Nicola N.A. Synthesis by mouse peritoneal cells of G-CSF, the differentiation inducer for myeloid leukemia cells: Stimulation by endotoxin, M-CSF, and multi-CSF. Leuk. Res. 9:1985;35-50.
3. Nagata S., Golstein P. The FAS death factor. Science. 267:1995;1449-1456.
4. Bazan J. A novel family of growth factor receptor: A common binding domain in the growth hormone, prolactin, erythropoietin, and IL-6 receptors and the p75 IL-2 receptor beta chain. Biochem. Biophys. Res. Commun. 164:1989;788-795.
5. Miyajima A., Mui A. L.-F., Ogorochi T., Sakamaki K. Receptors for granulocyte-macrophage colony-stimulating factor, interleukin-3, and interleukin-5. Blood. 82:1993;1960-1974.
6. Mosely B., Beckmann M. P., March C. J., Idzera R. L., Gimpel S. K., Vandenbos T., Friend D., Alpert A., Ancerson D., Jackson J., Wignall J. M., Smith C., Gallis B., Sims J. E., Urdal D., Widmer M. B., Cosman D., Park L. S. The murine interleukin-4 receptor: Molecular cloning and characterization of secreted and membrane bound forms. Cell. 59:1989;335-348.
7. Goodwin R. G., Friend D., Ziegler S. F., Jerzy R., Falk B. A., Gimpel S. K., Cosman D., Dower S. K., March G. J., Namen A. E., Park L. S. Cloning of the human and murine interleukin-7 receptor: Demonstration of a soluble form and homology to a receptor superfamily. Cell. 60:1990;941-951.
8. Cosman D. The hematopoietin receptor superfamily. Cytokine. 5:1993;95-106.
9. Bazan J. F. Structural design and molecular evolution of a cytokine receptor superfamily. Proc. Natl. Acad. Sci. USA. 87:1990;6934-6938.
10. Fukunaga R., Ishizaka-Ikeda E., Pan C.-X., Seto Y., Nagata S. Functional domains of the granulocyte colony-stimulating factor receptor. EMBO J. 10:1991;2855-2865.
11. Hiraoka O., Anaguchi H., Yamasaki K., Fukunaga R., Nagata S., Ota Y. Ligand binding domain of granulocyte colony-stimulating factor receptor. J. Biol. Chem. 269:1994;22412-22419.
12. Hiraoka O., Anaguchi H., Asakura A., Ota Y. Requirement for the immunoglobulin-like domain of granulocyte colony-stimulating factor receptor in formation of a 2:1 receptor-ligand complex. J. Biol. Chem. 270:1995;25928-25934.
13. Horan T., Wen J., Narhi L., Parker V., Garcia A., Arakawa T., Philo J. Dimerization of the extracellular domain of granulocyte-colony stimulating factor by ligand binding: A monovalent ligand induces 2:2 complex. Biochemistry. 35:1996;4886-4896.
14. Horan T., Martin F., Simonet L., Arakawa T., Philo J. Dimerization of granulocyte-colony stimulating factor receptor: The Ig plus CRH domain forms a 2:2 complex with ligand. J. Biochem. 121:1997;370-375.
15. Haniu M., Horan T., Arakawa T., Le J., Katta V., Hara S., Rohde M. F. Disulfide structure and N-glycosylation sites of an extracellular domain of granulocyte-colony stimulating factor receptor. Biochemistry. 35:1996;13040-13046.
16. Law M.-F., Byrne J. C., Howley P. M. A stable bovine papillomavirus hybrid plasmid that expresses a dominant selective trait. Mol. Cell. Biol. 3:1983;2110-2115.
17. Wigler M., Silverstein S., Lee L. S., Pellicer A., Chang Y. C., Axel R. Transfer of purified Herpes virus thymidine kinase gene to cultured mouse cells. Cell. 11:1977;223-232.
18. Wen J., Arakawa T., Philo J. Size-exclusion chromatography with on-line light scattering, absorbance, and refractive index detectors for studying proteins and their interactions. Anal. Biochem. 240:1996;155-156.
19. Denning G. M., Anderson M. P., Amara J. F., Marshall J., Smith A. E., Welsh M. J. Processing of mutant cystic fibrosis transmembrane conductance regulator is temperature-sensitive. Nature. 358:1992;761-764.
20. Komar A. A., Kommer A., Krasheninnikov I. A., Spirin A. S. Cotranslational folding of globin. J. Biol. Chem. 272:1997;10646-10651.
21. Walter P., Blobel G. Signal recognition particle: A ribo nucleoprotein required for cotranslational translocation of proteins; isolation and properties. Methods Enzymol. 96:1983;682-691.
22. Larsen A., Davis T., Curtis B. M., Gimpel S., Sims J. E., Cosman D., Park L., Sorensen E., March C. J., Smith C. A. Expression cloning of a human granulocyte colony-stimulating factor receptor: A structural mosaic of hematopoietin receptor, immunoglobulin, and fibronectin domains. J. Exp. Med. 172:1990;1559-1570.
23. Flynn G. C., Pohl J., Flocco M. T., Rothman J. E. Peptide-binding specificity of the molecular chaperone Bip. Nature. 353:1991;726-730.
24. Degen E., Williams D. B. Participation of a novel 88kD protein in the biogenesis of murine class I histocompatibility molecules. J. Cell. Biol. 112:1991;1099-1115.
25. Wada I., Rindress D., Cameron P. H., Ou W.-J., Doherty J. J., Louvard D., Bell A. W., Dignard D., Thomas D. Y., Bergeron J. J. M. SSRα and associated calnexin are major calcium binding proteins of the endoplasmic reticulum membrane. J. Biol. Chem. 266:1991;19599-19610.
26. Hammond C., Helenius A. Folding of VSV G protein: Sequential interaction with Bip and calnexin. Science. 266:1994;456-458.
27. Chen W., Helenius J., Braakman I., Helenius A. Cotranslational folding and calnexin binding during glycoprotein synthesis. Proc. Natl. Acad. Sci. USA. 92:1995;6229-6233.