Human N-Acetylglucosaminyltransferase I. Expression in Escherichia coli as a soluble enzyme, and application as an immobilized enzyme for the chemoenzymatic synthesis of N-linked oligosaccharides

Journal of Bioscience and Bioengineering

Volumn 92, Issue 6, 2001, Pages 569-574

  Fujiyama, Kazuhito ^a   Ido, Yoshihiro ^a   Misaki, Ryo ^a   Moran, Daniel G ^a   Yanagihara, Itaru ^b   Honda, Takeshi ^b   Nishimura, Shin Ichiro ^c   Yoshida, Toshiomi ^a   Seki, Tatsuji ^a  

^a OSAKA UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c HOKKAIDO UNIVERSITY   (Japan)

Author keywords

Acetylglucosaminyltransferase; Chemoenzymatic synthesis; Immobilization; N linked oligosaccharide

Indexed keywords

CATALYSIS; ENZYMES; ESCHERICHIA COLI; PROTEINS; SOLUBILITY; SYNTHESIS (CHEMICAL);

OLIGOSACCHARIDES;

BIOMEDICAL ENGINEERING;

AMYLOSE; MALTOSE BINDING PROTEIN; MANNOSE; N ACETYLGLUCOSAMINYLTRANSFERASE; OLIGOSACCHARIDE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CARBOHYDRATE SYNTHESIS; CATALYSIS; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; HUMAN; NONHUMAN; PROTEIN EXPRESSION;

ESCHERICHIA COLI; POSIBACTERIA;

EID: 0035702233     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(01)80318-2     Document Type: Article

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