Production and characterization of active soluble human β1,4-galactosyltransferase in Escherichia coli as a useful catalyst in synthesis of the Gal β1→4 G1cNAc linkage

Journal of Bioscience and Bioengineering

Volumn 91, Issue 1, 2001, Pages 85-87

  Shibatani, Shigeo ^a   Fujiyama, Kazuhito ^b   Nishiguchi, Susumu ^a   Seki, Tatsuji ^b   Maekawa, Yoshihiko ^a  

^a TOYOBO CO LTD   (Japan)

^b OSAKA UNIVERSITY   (Japan)

Author keywords

Escherichia coli; Maltose binding protein; 1,4 galactosyltransferase

Indexed keywords

GALACTOSE; GALACTOSYLTRANSFERASE; MALTOSE BINDING PROTEIN; OLIGOSACCHARIDE; URIDINE DIPHOSPHATE GALACTOSE;

ARTICLE; CATALYST; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SYNTHESIS;

BOVINAE; ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035109879     PISSN: 13891723     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1389-1723(01)80117-1     Document Type: Article

References (13)

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7. Secretion cloning vectors in Escherichia coli
8. Expression of mature pokeweed antiviral protein with or without C-terminal extrapeptide in Escherichia coli as a fusion with maltose-binding protein
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10. Studies on galactosyltransferase. Kinetic effects of α-lactalbumin with N-acetylglucosamine and glucose as galactosyl group acceptors
11. Analysis of the substrate binding sites of human galactosyltransferase by protein engineering
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13. Expression of deletion constructs of bovine beta-1,4-galactosyltransferase in Escherichia coli: Importance of Cys134 for its activity