Use of the mycobacteriophage L5 excisionase in Mycobacterium tuberculosis to demonstrate gene essentiality

Tuberculosis

Volumn 81, Issue 5-6, 2001, Pages 359-364

  Parish, T ^a   Lewis, J ^b   Stoker, N G ^a  

^a LONDON SCHOOL OF HYGIENE AND TROPICAL MEDICINE   (United Kingdom)

^b UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; EXCISIONASE; GENE PRODUCT; PROTEIN GLNE; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; BACTERIAL CHROMOSOME; BACTERIAL GENETICS; BACTERIOPHAGE; BACTERIUM COLONY; CONTROLLED STUDY; EXCISION; EXPRESSION VECTOR; GENE INACTIVATION; GENE TECHNOLOGY; GENETIC ANALYSIS; GENETIC RECOMBINATION; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PLASMID; PRIORITY JOURNAL;

MYCOBACTERIUM TUBERCULOSIS;

EID: 0035700179     PISSN: 14729792     EISSN: None     Source Type: Journal    
DOI: 10.1054/tube.2001.0312     Document Type: Article

References (9)

1. glnE is an essential gene in Mycobacterium tuberculosis
2. Production of mutants in amino acid biosynthesis genes of Mycobacterium tuberculosis by homologous recombination
3. Site-specific integration of mycobacteriophage L5 - Integration proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and Bacille Calmette-Guerin
4. Identification and characterization of mycobacteriophage L5 excisionase
5. Isolation of genomic DNA from mycobacteria
6. Use of a flexible cassette method to generate a double unmarked Mycobacterium tuberculosis tlyA plcABC mutant by gene replacement
7. Superinfection immunity of mycobacteriophage L5: Applications for genetic transformation of mycobacteria
8. An essential two-component signal transduction system in Mycobacterium tuberculosis
9. New use of BCG for recombinant vaccines