Identification and characterization of mycobacteriophage L5 excisionase

Molecular Microbiology

Volumn 35, Issue 2, 2000, Pages 350-360

  Lewis, John A ^a   Hatfull, Graham F ^a  

^a UNIVERSITY OF PITTSBURGH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; EXCISIONASE; INTEGRASE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIOPHAGE; CONTROLLED STUDY; DNA RECOMBINATION; ENZYME SPECIFICITY; GENOME; MYCOBACTERIUM SMEGMATIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROPHAGE; PROTEIN EXPRESSION; THERMOSTABILITY;

AMINO ACID SEQUENCE; ATTACHMENT SITES, MICROBIOLOGICAL; DNA NUCLEOTIDYLTRANSFERASES; DNA, VIRAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MOLECULAR SEQUENCE DATA; MYCOBACTERIOPHAGES; MYCOBACTERIUM SMEGMATIS; PLASMIDS; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE ALIGNMENT; VIRAL PROTEINS; VIRUS INTEGRATION;

EID: 0033972688     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01695.x     Document Type: Article

References (34)

1. Argos, P., Landy, A., Abremski, K., Egan, J.B., Haggard-Ljungquist, E., Hoess, R.H., et al. (1986) The integrase family of site-specific recombinases: regional similarities and global diversity. EMBO J 5: 433-440.
2. Ausubel, P.M., Brent, R., Kingston, R.E., Moore, D.D., Smith, U.A., Seidman, J.G., and Struhl, K. (1988) Current Protocols in Molecular Biology Brooklyn: Green Publishing Associates, p. 2.4.2.
3. Banerjee, A., Dubnau, E., Quemard, A., Balasubramanian, V., Um, K.S., Wilson, T., et al. (1994) inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis. Science 263: 227-230.
4. Barsom, E.K., and Hatfull, G.F. (1996) Characterization of a Mycobacterium smegmatis gene that confers resistance to phages L5 and D29 when overexpressed. Mol Microbiol 21: 159-170.
5. Brown, K.L., Sarkis, G.J., Wadsworth, C., and Hatfull, G.F. (1997) Transcritional silencing by the mycobacteriophage L5 repressor. EMBO J 16: 5914-5921.
6. Cole, S. T., Brosch, R., Parkhill, J., Gamier, T., Churcher, C., Harris, D., et al. (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393: 537-544.
7. Esposito, D., and Scocca, J.J. (1997) The integrase family of tyrosine recombinases: evolution of a conserved active site domain. Nucleic Acids Res 25: 3605-3614.
8. Ford, M.E., Sarkis, G.J., Belanger, A.E., Hendrix, R.W., and Hatfull, G.F. (1998) Genome strucure of mycobacteriophage D29: implications for phage evolution. J Mol Biol 279: 143-164.
9. Fullner, K.J., and Hatfull, G.F. (1997) Mycobacteriophage L5 infection of Mycobacterium bovis BCG: implications for phage genetics in the slow growing mycobacteria. Mol Microbiol 26: 755-766.
10. Hatfull, G.F. (1994) Mycobacteriophage L5: a toolbox for tuberculosis. Am Soc Microbiol News 60: 255-260.
11. Hatfull, G.F., and Grindley, N.D.F. (1988) Resolvases and DNA-lnvertases: a Family of Enzymes Active in Site-Specifc Recombination. In Genetic Recombination. Kucherlapati, R., and Smith, G.R. (eds). Washington, DC: American Society for Microbiology, pp. 357-296.
12. Hatfull, G.F., and Jacobs Jr, W.R. (1994) Mycobacteriophages: Cornerstones of mycobacterial research. In Tuberculosis: Pathogenesis, Protection and Control. Bloom B. R. (ed.). Washington, DC: American Society for Microbiology, pp. 165-183.
13. Hatfull, G.F., and Sarkis, G.J. (1993) DNA sequence, structure and gene expression of mycobacteriophage L5: a phage system for mycobacterial genetics. Mol Microbiol 7: 395-405.
14. Hendrix, R.W., Smith, M.C.M., Burns, N., Ford, M.E., and Hatfull, G.F. (1999) Evolutionary relationships among diverse bacteriophages and prophages: all the World's a Phage. Proc Natl Acad Sci USA 96: 2192-2197.
15. Landy, A. (1989) Dynamic, structural and regulatory aspects of λ site-specific recombination. Annu Rev Biochem 58: 913-949.
16. Lee, M.H., and Hatfull, G.F. (1993) Mycobacteriophage L5 integrase-mediated site-specific integration in vitro. J Bacteriol 175: 6836-6841.
17. Lee, M.H., Pascopella, L., Jacobs Jr, W.R., and Hatfull, G.F. (1991) Site-specific integration of mycobacteriophage L5: Integration-proficient vectors for Mycobacterium smegmatis, Mycobacterium tuberculosis, and bacille Calmette-Guérin. Microbiology 88: 3111 -3115.
18. Nesbit, C.E., Levin, M.E., Donnelly-Wu, M.K., and Hatfull, G.F. (1995) Transcriptional regulation of repressor synthesis in mycobacteriophage L5. Mol Microbiol 17 1045-1056.
19. Pascopella, L, Collins, F.M., Martin, J.M., Lee, M.H., Hatfull, G.F., Stover, C.K., et al. (1994) Use of in vivo complementation in Mycobacterium tuberculosis to identify a genomic fragment associated with virulence. Infect Immun 62: 1313-1309.
20. Pavelka, M.S., and Jacobs Jr, W.R. (1996) Biosynthesis of Diaminopimelate, the precursor of lysine and a component of the peptidoglycan, is an essential function of Mycobacterium smegmatis. J Bacteriol 178: 6496-6507.
21. Pedulla, M.L., and Hatfull, G.F. (1998) Characterization of the mlHF Gene of Mycobacterium smegmatis. J Bacteriol 180: 5473-5477.
22. Pedulla, M.L., Lee, M.H., Lever, D.C., and Hatfull, G.F. (1996) A novel host factor for integration of mycobacteriophage L5. Proc Natl Acad Sci USA 93: 15411-15416.
23. Pelicic, V., Reyrat, J.M., and Gicquel, B. (1996) Generation of unmarked directed mutations in mycobacteria, using sucrose counter-selectable suicide vectors. Mol Microbiol 20: 919-925.
24. Pen̄a, C.E.A., Stoner, J.E., and Hatfull, G.F. (1996) Position of strand exchange in mycobacteriophage L5 integration and characterization of the attB Site. J Bacteriol 178: 5533-5536.
25. Pen̄a, C.E.A., Lee, M.H., Pedulla, M.L., and Hatfull, G.F. (1997) Characterization of the mycobacteriophage L5 attatchment site attP. J Mol Biol 266: 76-92.
26. Peña, C.E.A., Stoner, J., and Hatfull, G.F. (1998a) Mycobacteriophage D29 integrase-mediated recombination: specificity of mycobacteriophage integration. Gene 225: 143-151.
27. Peña, C.E.A., Kahlenberg, J.M., and Hatfull, G.F. (1998b) The role of supercoiling in mycobacteriophage L5 integrative recombination. Nucleic Acids Res 26: 4012-4018.
28. Peña, C.E.A., Kahlenberg, M., and Hatfull, G.F. (1999) Protein-DNA complexes in mycobacteriophage L5 integrative recombination. J Bacteriol 181: 454-461.
29. Ptashne, M. (1992) A Genetic Switch: Gene Control and Phage λ. Palo Alto, CA: Cell Press & Blackwell Scientific Publications, pp. 49-67.
30. Salmi, D., Magrini, V., Hartzell, P.L., and Youderian, P. (1998) Genetic determinants of immunity and integration of temperate Myxococcus xanthus phage Mx8. J Bacteriol 180: 614-621.
31. Schägger, H., and Jagow, G.V. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electorphoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166: 368-379.
32. Snapper, S.B., Lugosi, L, Jekkel, A., Melton, R.E., Kieser, T., Bloom, B.R., and Jacobs Jr, W.R. (1988) Lysogeny and transformation in mycobacteria: stable expression of foreign genes. Proc Natl Acad Sci USA 85: 6987-6991.
33. Stover, C.K., de la Cruz, V.F., Fuerst, T.R., Burlein, J.E., Benson, LA., Bennett, L.T., et al. (1991) New use of BCG for recombinant vaccines. Nature 351: 456-160.
34. Thorpe, H.M., arid Smith, M.C. (1998) In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc Natl Acad Sci USA 95: 5505-5510.