Green Fluorescent Protein as a Novel Indicator of Antimicrobial Susceptibility in Aureobasidium pullulans

Applied and Environmental Microbiology

Volumn 67, Issue 12, 2001, Pages 5614-5620

  Webb, Jeremy S ^a   Barratt, Sarah R ^a   Sabev, Hristo ^a   Nixon, Marianne ^b   Eastwood, Ian M ^b   Greenhalgh, Malcolm ^b   Handley, Pauline S ^a   Robson, Geoffrey D ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b Avecia Biologics Limited   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DYES, REAGENTS, INDICATORS, MARKERS AND BUFFERS; FUNGICIDE; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN;

ARTICLE; ASCOMYCETES; DRUG EFFECT; FUNGUS SPORE; GENETIC TRANSFORMATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; SPECTROFLUOROMETRY;

ASCOMYCOTA; FUNGICIDES, INDUSTRIAL; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MICROBIAL SENSITIVITY TESTS; SPECTROMETRY, FLUORESCENCE; SPORES, FUNGAL; SUPPORT, NON-U.S. GOV'T; TRANSFORMATION, GENETIC;

EID: 0035650932     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.12.5614-5620.2001     Document Type: Article

References (43)

1. Baldwin, B. C., and T. E. Wiggins. 1484. Action of fungicidal triazoles of the dichlorobutrazol series un Ustilago maylis. Pesticide Sci. 15:156-166.
2. Ben-Josef, A. M., E. K. Manavathu, D. Platt, and J. D. Sobel. 2000. Proton translocating ATPase mediated fungicidal activity of a novel complex carbohydrate: CAN-296. Int. J. Antimicrob. Agents 13:287-295.
3. Casey, W. M., and N. T. Nguyen. 1995. Use of the green fluorescent protein to rapidly assess viability of Escherichia coli in preserved solutions. PDA J. Pharm. Sci. Technol. 50:352-355.
4. Chalfie, M., Y. Tu, G. Euskirchen, W. W. Ward, and D. C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science 263:802-805.
5. Cheng, L., J. Fu, A. Tsukamnto, and R. G. Hawley. 1996. Use of green fluorescent protein variants to monitor gene transfer and expression in mammalian cells. Nat. Biotechnol. 14:606-609.
6. Collins, L. A., M. N. Torrero, and S. G. Franzblau. 1998. Green fluorescent protein reporter microplate assay for high-throughput screening of compounds against Mycobacterium tuberculosis. Antimicrob. Aqents Chemother. 42:344-347.
7. Cormack, B. P., G. Bertram, M. Egerton, N. A. R, Gow, S. Falkow, and A. J. P. Brown. 1997. Yeast-enhanced green fluorescent protein (yEGFP): a reporter of gene expression in Candida albicans. Microbiology 143:303-311.
8. Costerton, J. W., P. S. Stewart, and E. P. Greenberg. 1999. Bacterial biofilms: a common cause of persistent infections. Science 284:1318-1322.
9. Cubitt, A. B., R. Heim, S. R. Adams, A. E. Boyd, L. A. Gross, and R. Y. Tsien. 1995. Understanding, improving and using green fluorescent proteins. Trends Biochem. Sci. 20:448-455.
10. Dhandayuthapani, S. L., E. Via, C. A. Thomas, P. M. Horowitz, D. Deretic, and V. Deretic. 1995. Green fluorescent protein as a marker for gene expression and cell biology of mycobacterial interactions with macrophages. Mol. Microbiol. 17:901-912.
11. Duncan, S., L. A. Glover, K. Killham, and J. I. Prosser. 1994. Luminescence-based detection of activity of starved and viable but nonculturable bacteria. Appl. Environ. Microbiol. 60:1308-1316.
12. Ermolayeva, E., and D. Sanders. 1995. Mechanism of pyrithione-induced membrane depolarization in Neurospora crassa. Appl. Environ. Microbiol. 61:3385-3390.
13. Gordon, C. L., V. Khalaj, A. F. J. Ram, D. B. Archer, J. L. Brookman, A. P. J. Trinci, D. J. Jeenes, J. H. Doonan, B. Wells, P. J. Punt, C. A. M. J. J. van den Hondel, and G. D. Robson. 2000. Glucoamylase::green fluorescent protein fusions to monitor protein secretion in Aspergillus niger. Microbiology 146: 415-426.
14. Hamilton, N. F. 1983. Biodeterioration of flexible polyvinyl chloride films by fungal organisms, p. 663-678. In T. A. Oxley and S. Barry (ed.), Biodeterioration 5. John Wiley & Sons Lid., Chichester, United Kingdom.
15. Heim, R., and R. Y. Tsien. 1996. Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer. Curr. Biol. 6:176-182.
16. Imai, T., and T. Ohno. 1995. The relationship between viability and intracellular pH in the yeast Saccharomyces cerevisiae. Appl. Environ. Microbiol. 42:344-347.
17. Kerr, C. J., C. R. Jones, V. F. Hillier. G. D. Robson, K. S. Osborn, and P. S. Handley. 1999. Statistical evaluation of a newly modified Robbins device using a bioluminescent pseudomonad to quantify adhesion to plastic. Biofouling 14:267-278.
18. Kneen, M., J. Farinas, Y. Li, and A. S. Verkman. 1998. Green fluorescent protein as a non-invasive intracellular pH indicator. Biophys. J. 74:1591-1599.
19. Kremer, L., A. Baulard, J. Estaquier, O. Poulain-Godefroy, and C. Locht. 1995. Green fluorescent protein as a new expression marker in mycobacteria. Mol. Microbiol. 17:913-922.
20. Llopis, J., J. M. McCaffery, A. Miyawaki, M. G. Farquhar, and R. Y. Tsien. 1998. Measurement of cytosolic, mitochondrial and Golgi pH in single living cells with green fluorescent proteins. Proc. Natl. Acad. Sci. USA 95:6803-6808.
21. 2+ based on green fluorescent proteins and calmodulin. Nature 388:882-887.
22. Monk, B. C., and D. S. Perlin. 1994. Fungal plasma membrane proton pumps as promising new antifungal targets. Crit. Rev. Microbiol. 20:209-223.
23. Noshiro, A., C. Purwin, M. Laux, K. Nicolay, W. A. Scheffers, and H. Holzer. 1987. Mechanism of stimulation of endogenous fermentation in yeast by carbonyl cyanide m-chlorophenylhydrazone. J. Biol. Chem. 262:14154-14157.
24. Parton, R. M., S. Fischer, R. Malhó. O. Papasouliotis, T. C. Jelilto, T. Leonard, and N. D. Read. 1997. Pronounced cytoplasmic gradients are not required for tip growth in plant and fungal cells. J. Cell Sci. 110:1187-1198.
25. Prasher, D. C., V. K. Eckenrode, W. W. Ward, F. G. Prendergast, and M. J. Cormier. 1992. Primary structure of the Aequoria victoria green fluorescent protein. Gene 111:229-233.
26. Punt, P. J., R. P. Oliver, M. A. Dingemanse, P. H. Pouwels, and C. A. M. J. J. van den Hondel. 1987. Transformation of Aspergillus based on the hygromycin B resistance marker from Escherichia coli. Gene 56:117-124.
27. Robey, R. B., O. Ruiz, A. V. P. Santos, J. Ma, F. Kear, L. Wang, C. Li, A. A. Bernardo, and J. A. L. Arruda. 1998. pH-dependent fluorescence of a heterologously expressed Aequoria green fluorescent protein mutant: in situ spectral characteristics and applicability to intracellular pH estimation. Biochemistry 37:9894-9901.
28. Rodriguez, G. G., D. Phipps, K. Ishiguro, and H. F. Ridgway. 1992. Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Appl. Environ. Microbiol. 58:1801-1808.
29. Serrano, R. M. 1988. Structure and function of a proton translocating ATPase in the plasma membranes of plants and fungi. Biochim. Biophys. Acta 947:1-28.
30. 2+ -ATPase. Nature 319:689-693.
31. Sheen, J., S. Hwang, Y. Niwa, H. Kobayaski, and D. W. Galbraith. 1995. Green-fluorescent protein as a new vital marker in plant cells. Plant J. 8:777-784.
32. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
33. Stewart, G. S. A. B., and P. Williams. 1992. lux genes and the applications of bacterial bioluminescence. J. Gen. Microbiol. 138:1289-1300.
34. Swaminathan, R., C. P. Hoang, and A. S. Verkman. 1997. Photobleaching recovery and anisotropy decay of green fluorescent protein GFP-S65T in solution and in cells: cytoplasmic viscosity probed by green fluorescent protein translational and rotational diffusion. Biophys. J. 72:1900-1907.
35. Trueman, J. R. 1971. The halogens, p. 138-183. In W. B. Hugo (ed.), Inhibition and destruction of the microbial cell. Academic Press, London, England.
36. Tsien, R. Y. 1998. The green fluorescent protein. Annu. Rev. Biochem. 67:509-544.
37. Vanden Wymelenberg, A. J., D. Cullen, R. N. Spear, B. Schoenike, and J. H. Andrews. 1997. Expression of green fluorescent protein in Aureobasidium pullulans and quantification of the fungus on leaf surfaces. BioTechniques 23:686-690.
38. +-ATPase in the plasma membrane of Saccharomyces cerevisiae is activated during growth latency in octanoic acid-supplemented medium accompanying the decrease in intracellular pH and cell viability. Appl. Environ. Microbiol. 64:779-783.
39. Wang, J., D. W. Holden, and S. A. Leong. 1988. Gene transfer system for the phytopathogenic fungus Ustilago maydis. Proc. Natl. Acad. Sci. USA 85:865-869.
40. Webb, J. S., M. Nixon, I. M. Eastwood, M. Greenhalgh, G. D. Robson, and P. S. Handley. 2001. Fungal colonization and biodeterioration of plasticized polyvinyl chloride. Appl. Environ. Microbiol. 66:3014-3200.
41. Weitzel, G., U. Pilalus, and L. Rensing. 1987. The cytoplasmic pH, ATP content and total protein synthesis rate during heat-shock protein inducing treatments in yeast. Exp. Cell Res. 170:64-79.
42. Xu, K. D., G. A. McFeters, and P. S. Stewart. 2000. Biofilm resistance to antimicrobial agents. Microbiology 146:547-549.
43. Yang, H., Y. Nemoto, T. Momma, H. Matsuoka, S. Yamada, O. Sumita, K. Takatori, and H. Kurata. 1995. Rapid viability assessment of spores of several fungi by an ionic intensified fluorescein diacetate method. Curr. Microbiol. 30:173-176.