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Volumn 10, Issue 1, 2001, Pages 86-91

Bioavailability of phosphorus in low phytic acid barley

Author keywords

Barley; Bioavailability; Broiler; Low phytic acid; Phosphorus

Indexed keywords


EID: 0035612152     PISSN: 10566171     EISSN: None     Source Type: Journal    
DOI: 10.1093/japr/10.1.86     Document Type: Article
Times cited : (27)

References (21)
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    • A l-g sample of grain was mixed with 20 mL of 0.2M citrate buffer (pH 5.5) in 1M NaCl solution and extracted on a magnetic stirrer at 30°C for 30 min. The slurry was centrifuged, and the clear supernatant was cooled on an ice bath and served as a source of enzyme. One milliliter of enzyme solution was pipetted into 1 mL of 5 mM sodium phytate solution and incubated for 120 min. The reaction was stopped by the addition of 2 mL of 15% TCA. The concentration of P was determined by the standard Fiske Subbarow method. One unit of phytase activity was defined as the amount of enzyme that frees 1 μmol inorganic P from 1.5 mM sodium phytate/ min at pH 5.5 and 37°C
    • A l-g sample of grain was mixed with 20 mL of 0.2M citrate buffer (pH 5.5) in 1M NaCl solution and extracted on a magnetic stirrer at 30°C for 30 min. The slurry was centrifuged, and the clear supernatant was cooled on an ice bath and served as a source of enzyme. One milliliter of enzyme solution was pipetted into 1 mL of 5 mM sodium phytate solution and incubated for 120 min. The reaction was stopped by the addition of 2 mL of 15% TCA. The concentration of P was determined by the standard Fiske Subbarow method. One unit of phytase activity was defined as the amount of enzyme that frees 1 μmol inorganic P from 1.5 mM sodium phytate/ min at pH 5.5 and 37°C.
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* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.