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6
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0031875175
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Linkage mapping of two mutations that reduce phytic acid content of barley grain
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Larson, S.R., K.A. Young, A. Cook, T.K. Blake, and V. Raboy, 1998. Linkage mapping of two mutations that reduce phytic acid content of barley grain. Theor. Appl. Genet. 97:141-146.
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7
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0033556132
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Availability of phosphorus and trace elements in low-phytate varieties of barley and corn for rainbow trout (Oncorhynchus mykiss)
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0347592370
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Low phytic acid barley for growing pigs: Pig performance and bone strength
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Zurich, Switzerland
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Veum, T.L., D.R. Ledoux, and V. Raboy, 1999. Low phytic acid barley for growing pigs: Pig performance and bone strength. Page 287 In: Proc. 50th Annu. Mtg. Eur. Assoc. Anim. Prod., Zurich, Switzerland.
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10
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0346474456
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Stover Hatchery, Stover, MO.
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11
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0345843626
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USDA-Agricultural Research Service, 1691 S. 2700 W, P.O. Box 307, Aberdeen, ID 83210
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USDA-Agricultural Research Service, 1691 S. 2700 W, P.O. Box 307, Aberdeen, ID 83210.
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12
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0000286458
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0001349401
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0347104743
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Hoffmann-La Roche Inc., Nutley, NJ
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Hoffmann-La Roche Inc., Nutley, NJ.
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15
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0347104741
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A l-g sample of grain was mixed with 20 mL of 0.2M citrate buffer (pH 5.5) in 1M NaCl solution and extracted on a magnetic stirrer at 30°C for 30 min. The slurry was centrifuged, and the clear supernatant was cooled on an ice bath and served as a source of enzyme. One milliliter of enzyme solution was pipetted into 1 mL of 5 mM sodium phytate solution and incubated for 120 min. The reaction was stopped by the addition of 2 mL of 15% TCA. The concentration of P was determined by the standard Fiske Subbarow method. One unit of phytase activity was defined as the amount of enzyme that frees 1 μmol inorganic P from 1.5 mM sodium phytate/ min at pH 5.5 and 37°C
-
A l-g sample of grain was mixed with 20 mL of 0.2M citrate buffer (pH 5.5) in 1M NaCl solution and extracted on a magnetic stirrer at 30°C for 30 min. The slurry was centrifuged, and the clear supernatant was cooled on an ice bath and served as a source of enzyme. One milliliter of enzyme solution was pipetted into 1 mL of 5 mM sodium phytate solution and incubated for 120 min. The reaction was stopped by the addition of 2 mL of 15% TCA. The concentration of P was determined by the standard Fiske Subbarow method. One unit of phytase activity was defined as the amount of enzyme that frees 1 μmol inorganic P from 1.5 mM sodium phytate/ min at pH 5.5 and 37°C.
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17
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21
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0034303130
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