Visualization of meiotic events in intact living anthers by means of two-photon microscopy

Micron

Volumn 32, Issue 7, 2001, Pages 679-684

  Feijó, José A ^a,b   Cox, Guy ^c  

^a INSTITUTO GULBENKIAN DE CIÊNCIA   (Portugal)

^b UNIVERSITY OF LISBON   (Portugal)

^c UNIVERSITY OF SYDNEY   (Australia)

Author keywords

Agapanthus umbelatus; Anthers; DAPI; Meiosis; Two photon microscopy

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; INDOLE DERIVATIVE;

ANGIOSPERM; ARTICLE; FLUORESCENCE MICROSCOPY; MEIOSIS; METABOLISM; METHODOLOGY; PHOTON; PHYSIOLOGY; PLANT; ULTRASTRUCTURE;

ANGIOSPERMS; INDOLES; MEIOSIS; MICROSCOPY, FLUORESCENCE; PHOTONS; PLANT COMPONENTS;

AGAPANTHUS;

EID: 0035480577     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(00)00097-4     Document Type: Article

References (25)

1. Aragon-Alcaide L., Beven A., Moore G., Shaw P. The use of vibratome sections of cereal spikelets to study anther development and meiosis. Plant J. 14:1998;503-508.
2. Bai X., Peirson B.N., Dong F., Xue C., Makaroff C.A. Isolation and characterization of SYNI, aRAD21-like gene essential for meiosis in Arabidopsis. Plant Cell. 11:1999;417-430.
3. Bennet M.D., Smith J.B., Simpson S., Wells B. Intranuclear fibrillar material in cereals pollen mother cells. Chromosoma. 71:1979;289-332.
4. Chikashige, Y., Ding, D.-Q., Funabiki, H., Haraguchi, H., Haraguchi, T., Mashiko, S., Yanagida, M., Hiraoka, Y., 1994. Telomere-led premeitoic chromosome movement in fission yeast. Science 264, 270-273
5. Couteau F., Belzile F., Horlow C., Grandjean Vezon D., Doutriaux M.-P. Random chromosome segregation without meiotic arrest in both male and female meiocytes of a dmcl mutant of Arabidopsis. Plant Cell. 11:1999;1623-1634.
6. Cox G.C., Sheppard C.J.R. Multiphoton fluorescence microscopy. Mason W.T., Fluorescent and Luminescent Probes for Biological Activity. 2nd ed. :1999;331-336 Academic Press.
7. Dawe R.K., Sedat J.W., Agard D.A., Cande W.Z. Meiotic chromosome pairing in maize is associated with a novel chromatin organization. Cell. 76:1994;901-912.
8. Denk W., Piston D.W., Webb W.W. Two photon molecular excitation in laser scanning microscopy. Pawley J.B., Handbook of Biological Confocal Microscopy. 2nd ed. :1995;445-458 Plenum Press, New York.
9. Ding D-Q., Chikashige Y., Haraguchi T., Hiraoka Y. Oscillatory nuclear movement in fission yeast meiotic prophase is driven by astral microtubules, as revealed by continuous observation of chormosomes and microtubules in living cells. J. Cell Sci. 111:1998;701-712.
10. Endow S.A., Komma D.J. Spindle dynamics druring meiosis in Drosophila oocytes. J. Cell Biol. 137:1997;1321-1336.
11. Endow S.A., Komma D.J. Assembly and dynamics of an anastral:astral spindle: the meiosis II spindle of Drosophila oocytes. J. Cell Sci. 111:1998;2487-2495.
12. Feijó J.A., Pais M.S. Cytomixis in meiosis during the microsporogenesis of Ophrys lutea: an ultrastuctural study. Caryologia. 42:1989;37-48.
13. Feijó, J.A., 1995. Sexual reproduction in Ophrys lutea. PhD.Thesis, University of Lisboa, Lisboa
14. Franklin A.E., McElver J., Sunjevaric I., Rothstein R., Bowen B., Cande W.Z. Three-dimensional microscopy of the rad51 recombination protein during meiotic prophase. Plant Cell. 11:1999;808-824.
15. Gerritsen H.G., DeGrauw C.J. Imaging of optically thick specimen using two-photon excitation microscopy. Microsc. Res. Tech. 47:1999;206-209.
16. Heberle-Bors E. Experimental control of pollen development. Chupeau Y., Caboche M., Henry Y., Androgenesis and Haploid Plants. 1988;Springer-Verlag, Berlin, Heidelberg, New York.
17. Heslop-Harrison J. Cytoplasmic conections between angiosperms meiocytes. Ann. Bot. 30:1966;222-230.
18. Martinez-Perez E., Shaw P., Reader S., Aragon-Alcaide L., Miller T., Moore G. Homologous chromosome pairing in wheat. J. Cell Sci. 112:1999;1761-1769.
19. Miller A.L., Gow A.R. Correlation between profile of ion-current circulation and root development. Physiologia Plantarum. 75:1989;102-108.
20. Patterson G.H., Piston D.W. Photobleaching in two-photon excitation microscopy. Biophys. J. 78:2000;2159-2162.
21. Piston D.W., Knobel S. Quantitative imaging of metabolism by two-photon excitation microscopy. Methods in Enzymology. 307:1999;351-369.
22. Piston D.W., Summers R.G., Webb W.W. Biophysical Journal. 63:1993;A110.
23. Potter S. Vital imaging: two photons are better than one. Curr. Biol. 6:1996;1595-1598.
24. Reynolds T.L. Pollen embryogenesis. Plant Molecular Biology. 33:1997;1-10.
25. Yu H.-G., Muszynski M.G., Dawe R.K. The maize homolog of the cell cycle checkpoint protein MAD2 reveals kinetochore substructure and contrasting mitotic and meiotic localization patterns. J. Cell Biol. 145:1999;425-435.