5′-bis-pyrenylated oligonucleotides displaying excimer fluorescence provide sensitive probes of RNA sequence and structure

Nucleic Acids Research

Volumn 29, Issue 17, 2001, Pages 3611-3620

  Kostenko, E ^a   Dobrikov, M ^a   Pyshnyi, D ^a   Petyuk, V ^a   Komarova, N ^a   Vlassov, V ^a   Zenkova, M ^a  

^a INSTITUTE OF CHEMICAL BIOLOGY AND FUNDAMENTAL MEDICINE   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; MESSENGER RNA; MONOMER; MULTIDRUG RESISTANCE PROTEIN; OLIGONUCLEOTIDE; PHOSPHATE; PYRENE; RNA; GLYCOPROTEIN P; PYRENE DERIVATIVE;

ARTICLE; BINDING AFFINITY; BINDING KINETICS; BINDING SITE; CHEMICAL BOND; CONTROLLED STUDY; FLUORESCENCE; HUMAN; HYBRIDIZATION; PRENYLATION; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; RNA BINDING; RNA PROBE; RNA SEQUENCE; RNA STRUCTURE; SYNTHESIS; CHEMISTRY; CONFORMATION; DNA DENATURATION; DNA PROBE; GENETICS; MOLECULAR GENETICS; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; SPECTROFLUOROMETRY; TEMPERATURE;

BASE SEQUENCE; DNA PROBES; FLUORESCENCE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; NUCLEIC ACID DENATURATION; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDES; P-GLYCOPROTEIN; PYRENES; RNA; RNA, MESSENGER; SPECTROMETRY, FLUORESCENCE; TEMPERATURE;

EID: 0035437821     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/29.17.3611     Document Type: Article

References (43)

1. Progress in antisense technology: The end of the beginning
2. Effects of RNA secondary structure on cellular antisense activity
3. Implication of RNA structure on antisense oligonucleotide hybridisation kinetics
4. An in vitro messenger RNA binding assay as a tool for identifying hybridisation-competent antisense oligonucleotides
5. Combinatorial screening and rational optimisation for hybridisation to folded hepatitis C virus RNA of oligonucleotides with biological antisense activity
6. A rapid in vitro method for obtaining RNA accessibility patterns for complementary DNA probes: Correlation with an intracellular pattern and known RNA structures
7. Mapping of RNA accessible sites for antisense experiments with oligonucleotide libraries
8. Selecting effective antisense reagents on combinatorial oligonucleotide arrays
9. The folding of large RNAs studied by hybridisation to arrays of complementary oligonucleotides
10. Nucleic acid detection using non-radioactive labelling methods
11. Quantitative hybridisation kinetics of DNA probes to RNA in solution followed by diffusional fluorescence correlation analysis
12. The pyrene excimer
13. Novel reagent for labelling of biomolecules-activated derivative of pyrene dichromophore with excimer fluorescence
14. Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes
15. Bis-pyrenyl excimer fluorescence: A sensitive oligonucleotide probe
16. Oligonucleotides with bis-pyrene adduct in the backbone: Synthesis and properties of intramolecular excimer forming probe
17. Oligonucleotide-polyamide hybrid molecules containing multiple pyrene residues exhibit significant excimer fluorescence
18. Secondary structure of 5′-terminal region of mRNA of human multidrug resistance gene
19. Oligoribonucleotide synthesis using T7 RNA polymerase and synthetic DNA templates
20. The visualisation of human erythrocyte membrane proteins and glycoproteins in SDS polyacrylamide gels employing a single staining procedure
21. Design of functional diversity in oligonucleotides via zwitter-ionic derivatives of deprotected oligonucleotides
22. Sensitised photomodification by binary systems. I. Synthesis of oligonucleotide reagents, and the effect of their structure on the efficacy of target modification
23. Optimisation of excimer-forming two-probe nucleic acid hybridisation method with pyrene as a fluorophore
24. Phosphorylation by oxidation-reduction condensation. Preparation of novel phosphorylating reagents
25. S-S-Di-2-pyridyl di thiophosphate as a key intermediate in the phosphorylation by oxidation-reduction condensation
26. Stabilisation of DNA-duplexes by minor groove ligands forming complexes of 1:2 type with parallel ligands orientation in minor groove
27. Secondary structure of the 5′-region of PGY1/MDR1 mRNA
28. Phe
29. Length dependence of RNA-RNA annealing
30. The kinetics of oligonucleotide replacement
31. Binding of complementary oligonucleotides to free and aminoacyl transfer ribonucleic acid synthetase bound transfer ribonucleic acid
32. A novel approach to introduce site-directed specific cross-links within RNA-protein complexes. Application to the Escherichia coli threonyl-tRNA synthetase/translational operator complex
33. Base-sequence dependence of emission lifetimes for DNA oligomers and duplexes covalently labeled with pyrene: Relative electron-transfer quenching efficiencies of A, G and T nucleosides toward pyrene
34. Synthesis and properties of an oligodeoxynucleotide modified with a pyrene derivative at the 5′-phosphate
35. Synthesis and properties of oligonucleotides bearing a pendant pyrene group
36. Fluorescent-labelled oligonucleotides that exhibit a measurable signal in the presence of complementary DNA
37. Reagent for introducing pyrene residues in oligonucleotides
38. 2′-Pyrene modified oligonucleotide provides a highly sensitive fluorescent probe of RNA
39. Study on RNA structure by pyrene-labelled 2′-O-methyloligoribonucleotides
40. Concerning inhibition of multiple drug resistance genes
41. Overcoming multidrug resistance in human tumour cells using free and liposomally encapsulated antisense oligodeoxynucleotides