An in vitro messenger RNA binding assay as a tool for identifying hybridization-competent antisense oligonucleotides

Antisense and Nucleic Acid Drug Development

Volumn 6, Issue 3, 1996, Pages 221-228

  Stull, Robert A ^a   Zon, Gerald ^b   Szoka Jr , Francis C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b ILLUMINA INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGODEOXYNUCLEOTIDE; MESSENGER RNA; OLIGODEOXYNUCLEOTIDE PHOSPHOROTHIOATE; POLYACRYLAMIDE GEL; TUMOR NECROSIS FACTOR ALPHA;

ARTICLE; BINDING AFFINITY; CELL CULTURE; CONTROLLED STUDY; DISSOCIATION CONSTANT; DNA HYBRIDIZATION; ISOTOPE LABELING; PRIORITY JOURNAL; RNA BINDING; RNA TRANSCRIPTION;

MURINAE;

EID: 0029831281     PISSN: 10872906     EISSN: None     Source Type: Journal    
DOI: 10.1089/oli.1.1996.6.221     Document Type: Article

References (6)

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2. BACON, T.A., and WICKSTROM, E. (1991). Walking along the human c-myc mRNA with antisense oligodeoxynucleotides: Maximum efficacy at the 5′ cap region. Oncogene Res. 6, 13-19.
3. BILOFSKY, H.S., and BURKS, C. (1988). The GenBank genetic sequence data bank. Nucleic Acids Res. 16, 1861-1864.
4. BOIZIAU, C., KURFURST, R., CAZENAVE, C., ROIG, V., THUONG, N.T., and TOULMÉ, J.J. (1991). Inhibition of translation by antisense-oligonucleotides via an RNase-H independent mechanism. Nucleic Acids Res. 19, 1113-1119.
5. BORER, P.N. (1974). Optical properties of nucleic acids, absorption, and circular dichroism spectra. In: Handbook of Biochemistry and Molecular Biology. G. Fasman, ed. (CRC Press, Cleveland OH) pp. 843-853.
6. CAPUT, D., BEUTLER, B., HARTOG, K., THAYER, R., BROWN-SHIMER, S., and CERAMI, A. (1986). Identification of a common nucleotide sequence in the 3′-untranslated region of the mRNA molecules specifying inflammatory mediators. Proc. Natl. Acad. Sci. USA 83, 1670-1674.