Induction of a Mitosis Delay and Cell Lysis by High-Level Secretion of Mouse α-Amylase from Saccharomyces cerevisiae

Applied and Environmental Microbiology

Volumn 67, Issue 8, 2001, Pages 3693-3701

  Wang, Bi Dar ^a   Kuo, Tsong Teh ^a,b  

^a INSTITUTE OF MOLECULAR BIOLOGY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

[No Author keywords available]

Indexed keywords

AMYLASE; CDC55 PROTEIN, S CEREVISIAE; CELL CYCLE PROTEIN; CYCLIN DEPENDENT KINASE 1; FUNGAL DNA; MATURATION PROMOTING FACTOR; PHOSPHOPROTEIN PHOSPHATASE; RECOMBINANT PROTEIN; SACCHAROMYCES CEREVISIAE PROTEIN;

ANIMAL; ARTICLE; CULTURE MEDIUM; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; MITOSIS; MOUSE; PHYSIOLOGY; SACCHAROMYCES CEREVISIAE; ULTRASTRUCTURE;

ALPHA-AMYLASE; ANIMALS; CDC28 PROTEIN KINASE, S CEREVISIAE; CELL CYCLE PROTEINS; CULTURE MEDIA; DNA, FUNGAL; GENE EXPRESSION REGULATION, FUNGAL; MATURATION-PROMOTING FACTOR; MICE; MITOSIS; PHOSPHOPROTEIN PHOSPHATASE; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 0035432054     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.67.8.3693-3701.2001     Document Type: Article

References (56)

1. al-Rubeai, M., and A. N. Emery. 1990. Mechanisms and kinetics of monoclonal antibody synthesis and secretion in synchronous and asynchronous hybridoma cell cultures. J. Biotechnol. 16:67-86.
2. Alvarez, P., M. Sampedro, M. Molina, and C. Nombela. 1994. A new system for the release of heterologous proteins from yeast based on mutant strains deficient in cell integrity. J. Biotechnol. 8:81-88.
3. Bender, A., and J. R. Pringle. 1989. Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ras-related gene RSR1. Proc. Natl. Acad. Sci. USA 86:9976-9980.
4. Booher, R. N., R. J. Deshaies, and M. W. Kirschner. 1993. Properties of Saccaromyces cerevisiae weel and its differential regulation of p34CDC28 in response to G1 and G2 cyclins. EMBO J. 12:3417-3426.
5. Chen, D.-C., B.-C. Yang, and T.-T. Kuo. 1992. One-step transformation of yeast in stationary phase. Curr. Genet. 21:83-84.
6. Chen, D.-C., L.-T. Chuang, W.-P. Chen, and T.-T. Kuo. 1995. Abnormal growth induced by expression of HBsAg in the secretion pathway of S. cerevisiae pep4 mutants. Curr. Genet. 27:201-206.
7. Chen, D.-C., S.-Y. Chen, M.-F. Gee, J.-T. Pan, and T.-T. Kuo. 1999. A variant of Saccharomyces cerevisiae pep4 strain with improved oligotrophic proliferation, cell survival and heterologous secretion of alpha-amylase. Appl. Microbiol. Biotechnol. 51:185-192.
8. Chen, D.-C., B.-D. Wang, P.-Y. Chou, and T.-T. Kuo. 2000. Asparagine as nitrogen source for improving the secretion of mouse α-amylase in the Saccharomyces cerevisiae protease A deficient strains. Yeast 16:207-217.
9. Cherlet, M., S. J. Kromenaker, and F. Srienc. 1995. Surface IgG content of murine hybridomas: direct evidence for variation of antibody secretion rates during the cell cycle. Biotechnol. Bioeng. 47:535-540.
10. Cohen, P., S. Alemany, B. A. Hemmings, T. J. Resink, P. Stralfors, and H. Y. L. Tung. 1988. Protein phosphatase-1 and protein phosphatase 2A from rabbit skeletal muscle. Methods Enzymol. 15:390-408.
11. Drgonova, J., T. Drgon, K. Kollar, G. C. Chen, R. A. Ford, C. S. Chan, Y. Takai, and E. Cabib. 1996. Rholp, a yeast protein at the interface between cell polarization and morphogenesis. Science 272:277-279.
12. Errede, B., and D. E. Levin. 1993. A conserved kinase cascade for MAP kinase activation in yeast. Curr. Biol. 5:254-260.
13. Evangelista, C. C., A. M. Rodriguez Torres, M. P. Limbach, and R. S. Zitomer. 1996. Rox3 and Rtsl function in the global stress response pathway in baker's yeast. Genetics 142:1083-1093.
14. Evans, D. R. H., and M. J. R. Stark. 1997. Mutations in Saccharomyces cerevisiae type 2A protein phosphatase catalytic subunit reveal roles in cell wall integrity, actin cytoskeleton organization and mitosis. Genetics 145:227-241.
15. Fitch, I., C. Dahmann, U. Surana, A. Amon, K. Nasmyth, L. Goethsch, B. Bayers, and B. Futcher. 1992. Characterization of the B-type cyclin genes of the budding yeast Saccharomyces cerevisiae. Mol. Biol. Cell 3:805-818.
16. Ghiara, J. B., H. E. Richardson, K. Sugimoto, M. Henze, D. J. Lew, C. Wittenberg, and S. I. Reed. 1991. A cyclin B homolog in S. cerevisiae: chronic activation of the Cdc28 protein kinase by cyclin prevents exit from mitosis. Cell 65:163-174.
17. Gomes, R., R. E. Karess, H. Ohkura, D. M. Glover, and C. E. Sunkel. 1993. Abnormal anaphase resolution (aar): a locus required for progression through mitosis in Drosophila. J. Cell Sci. 104:583-593.
18. Hall, A. 1994. Small GTP-binding proteins and the regulation of actin cytoskeleton. Annu. Rev. Cell Biol. 10:31-54.
19. Healy, A. M., S. Zolnierowicz, A. E. Stapleton, M. Goebl, A. A. DePaoli-Roach, and J. R. Pringle. 1991. CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization, and homology to the B subunit of mammalian type 2A protein phosphatase. Mol. Cell. Biol. 11:5767-5780.
20. Helliwell, S. B., I. Howald, N. Barbet, and M. N. Hall. 1998. Evidence for two related TOR2 signaling pathways coordinating cell growth in Saccharomyces cerevisiae. Genetics 48:99-112.
21. Hoffmann, I., and E. Karsenti. 1994. The role of cdc25 in checkpoints and feedback controls in the eukaryotic cell cycle. J. Cell Sci. 18:75-79.
22. 1 cyclin expression in the budding yeast Saccharomyces cerevisiae. Mol. Cell. Biol. 13:6274-6282.
23. Kim, K., C. S. Park, and J. R. Mattoon. 1988. High-efficiency, one-step starch utilization by transformed Saccharomyces cerevisiae cells which secrete both yeast glucoamylase and mouse α-amylase. Appl. Environ. Microbiol. 54:966-971.
24. Kim, Y.-J., L. Francisco, G.-C. Chen, E. Marcotte, and C. S. M. Chan. 1994. Control of cellular morphogenesis by Ipl2/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J. Cell Biol. 127:1381-1394.
25. Kromenaker, S. J., and F. Srienc. 1991. Cell-cycle dependent protein accumulation by procedure and nonprocedure murine hybridoma cell lines: a population analysis. Biotechnol. Bioeng. 38:665-677.
26. Leberer, E., C. Wu, T. Leeuw, A. Fourest-Lieuvin, J. E. Segall, and D. Y. Thomas. 1997. Functional characterization of the Cdc42p binding domain of yeast Ste20p protein kinase. EMBO J. 16:83-97.
27. Lin, F. C., and K. T. Arndt. 1995. The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J. 14:2745-2759.
28. Martens, D. E., C. D. de Gooijer, C. A. M. van der Velden-de Groot, E. C. Beuvery, and J. Tramper. 1993. Effect of dilution rate on growth, productivity, cell cycle and size, and shear sensitivity of hybridoma cells in a continuous culture. Biotechnol. Bioeng. 41:429-439.
29. Mayer-Jaekel, R. E., H. Ohkura, P. Ferrigno, N. Andjelkovic, K. Shiomi, T. Uemura, D. M. Glover, and B. A. Hemmings. 1994. Drosophila mutants in the 55 kDa regulatory subunit of protein phosphatase 2A show strongly reduced ability to dephosphorylate substrates of p34cdc2. J. Cell Sci. 107:2609-2616.
30. Mendenhall, M. D., and A. E. Hodge. 1998. Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae. Microbiol. Mol. Biol. Rev. 62:1191-1243.
31. Minshull, J., A. Straight, A. D. Rudner, A. F. Dernburg, A. Belmont, and A. W. Murray. 1996. Protein phosphatase 2A regulates MPF activity and sister chromatid cohesion in budding yeast. Curr. Biol. 6:1609-1620.
32. Nanumiya, S., T. Ishizaki, and N. Watanabe. 1997. Rho effectors and reorganization of the actin cytoskeleton. FEBS Lett. 410:68-72.
33. Peterson, J., Y. Zheng, L. Bender, A. Myers, R. Cerione, and A. Bender. 1994. Interactions between the bud emergence protein Bemlp and Bem2p and Rho-type GTPases in yeast. J. Cell Biol. 127:1395-1406.
34. Piggot, J. R., R. B. Rai, and B. L. A. Carter. 1982. A bifunctional gene product involved in two phases of the yeast cell cycle. Nature 398:1228-1231.
35. Pines, J. 1995. Cyclins and cyclin-dependent kinases: theme and variations. Adv. Cancer Res. 66:181-212.
36. Qadota, H., C. P. Python, S. D. Inoue, M. Arisawa, Y. Anraku, Y. Zheng, T. Watanabe, D. E. Levin, and Y. Ohya. 1996. Identification of yeast Rholp GTPase as a regulatory subunit of 1,3-beta-glucan synthase. Science 272: 279-281.
37. Richardson, H. E., C. S. Stueland, J. Thomas, P. Russell, and S. I. Reed. 1992. Cyclin-B homologs in Saccharomyces cerevisiae function in S phase and G2. Genes Dev. 6:2021-2034.
38. Schwab, M., A. S. Lutum, and W. Seufert. 1997. Yeast Hct1 is a regulator of Clb2 cyclin proteolysis. Cell 90:683-693.
39. Skibbens, R. V., and P. Hieter. 1998. Kinetochores and the checkpoint mechanism that monitors for defects in the chromosome segregation machinery. Annu. Rev. Genet. 32:307-337.
40. Stateva, L. I., P. V. Venkov, A. A. Hadjiolov, L. A. Koleva, and N. L. Luydskanov. 1988. Polyploid fragile strains of S. cerevisiae - a novel source of proteins for nutritional purposes. Yeast 4:219-225.
41. Surana, U., H. Robitsch, T. Schuster, I. Fitch, A. B. Futcher, and K. Nasmyth. 1991. The role of CDC28 and cyclins during mitosis in the budding yeast S. cerevisiae. Cell 65:145-161.
42. Surana, U., A. Amon, C. Dowzer, J. McGrew, B. Byers, and K. Nasmyth. 1993. Destruction of CDC28/CLB mitotic kinase is not required for the metaphase to anaphase transition in budding yeast. EMBO J. 12:1969-1978.
43. Tapon, N., and A. Hall. 1997. Rho, Rac and Cdc42 GTPase regulate the organization of the actin cytoskeleton. Curr. Opin. Cell Biol. 9:86-92.
44. Toczyski, D. P., D. J. Galgoczy, and L. H. Hartwell. 1997. CDC5 and CK11 control adaptation to the yeast DNA damage checkpoint. Cell 90:1097-1106.
45. Uchiyama, K., M. Morimoto, Y. Yokoyama, and S. Shioya. 1998. Cell cycle dependency of rice α-amylase production in a recombinant yeast. Biotechnol. Bioeng. 54:262-271.
46. Uchiyama, K., and S. Shioya. 1999. Modeling and optimization of α-amylase production in a recombinant yeast fed-batch culture taking account of the cell cycle population distribution. J. Biotechnol. 71:133-141.
47. van Aelst, L., and C. D'Souza-Schorey. 1997. Rho GTPases and signaling networks. Genes Dev. 11:2295-2322.
48. van Zyl, W., W. Huang, A. A. Sneddon, M. Stark, S. Camier, M. Werner, C. Mark, A. Stentence, and J. R. Broach. 1992. Inactivatiori of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae. Mol. Cell. Biol. 12:4946-4959.
49. Verlhac, M.-H., R.-H. Chen, P. Hanachi, J. W. B. Hershey, and R. Derynck. 1997. Identification of partners of TIF34, a component of the yeast eIF3 complex, required for cell proliferation and translation initiation. EMBO J. 16:6812-6822.
50. Visintin, R., S. Prinz, and A. Amon. 1997. CDC20 and CDH1: a family of substrate-specific activators of APC-dependent proteolysis. Science 278:460-463.
51. Wang, B.-D., D.-C. Chen, and T.-T. Kuo. Characterization of a Saccharomyces cerevisiae mutant with oversecretion phenotype. Appl. Microbiol. Biotechnol., in press.
52. Wang, Y., and A. Bretscher. 1995. The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol. Biol. Cell 6:1011-1024.
53. Wang, Y., and D. J. Burke. 1997. Cdc55p, the B-type regulatory subunit of protein phosphatase 2A, has multiple functions in mitosis and is required for the kinetochore/spindle checkpoint in Saccharomyces cerevisiae. Mol. Cell. Biol. 17:620-626.
54. Wera, S., and B. A. Hemmings. 1995. Serine/threonine protein phosphatases. Biochem. J. 311:17-29.
55. Yanagida, M., N. Kinoshita, E. M. Stone, and H. Yamano. 1992. Protein phosphatases and cell division cycle control. Ciba Found. Symp. 170:130- 140.
56. Zhang, N., D. C. J. Gardner, S. G. Oliver, and L. I. Stateva. 1999. Genetically controlled cell lysis in the yeast Saccharomyces cerevisiae. Biotechnol. Bioeng. 64:607-615.