Mutations in the Saccharomyces cerevisiae type 2A protein phosphatase catalytic subunit reveal roles in cell wall integrity, actin cytoskeleton organization and mitosis

Genetics

Volumn 145, Issue 2, 1997, Pages 227-241

  Evans, David R H ^a   Stark, Michael J R ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIN; PHOSPHOPROTEIN PHOSPHATASE; SORBITOL;

ALLELE; CATALYSIS; CELL MEMBRANE PERMEABILITY; CELL PROLIFERATION; CYTOSKELETON; ENZYME ACTIVATION; GENE DELETION; GENE MUTATION; MITOSIS; MORPHOGENESIS; PRIORITY JOURNAL; REVIEW; SACCHAROMYCES CEREVISIAE; SEQUENTIAL ANALYSIS; TEMPERATURE SENSITIVITY;

ACTINS; ALLELES; CATALYSIS; CELL WALL; CYTOSKELETON; FUNGAL PROTEINS; MICROTUBULES; MITOSIS; MORPHOGENESIS; MUTATION; PHENOTYPE; PHOSPHOPROTEIN PHOSPHATASE; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS, DNA; TEMPERATURE;

SACCHAROMYCES CEREVISIAE;

EID: 0031014433     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Review

References (60)

1. ADAMS, A. E. M., and J. R. PRINGLE, 1984 Relationship of actin and tubulin distribution to bud growth in wild-type and morphogenetic mutant Saccharomyces cerevisiae. J. Cell Biol. 98: 934-945.
2. BARTON, G. J., P. T. W. COHEN and D. BARFORD, 1994 Conservation analysis and structure prediction of the serine/threonine phosphatases. Eur. J. Biochem. 220: 225-237.
3. BENDER, A., and J. R. PRINGLE, 1989 Multicopy suppression of the cdc24 budding defect in yeast by CDC42 and three newly identified genes including the ros-related gene RSR1. Proc. Natl. Acad. Sci. USA 86: 9976-9980.
4. BERBEN, G., J DUMONT, V. GILLIQUET, P.-A. BOLLE and F. HILGER, 1991 The YDp plasmids: a uniform set of vectors bearing versatile gene disruption cassettes for Saccharomyces cerevisiae. Yeast 7: 475-477.
5. BUTLER, A. R., J. H. WHITE and M. J. R. STARK, 1991 Analysis of the response of Saccharomyces cerevisiae cells to Kluyveromyces lactis toxin. J. Gen. Microbiol. 137: 1749-1757.
6. CHANT, J., 1994 Cell polarity in yeast. Trends Genet. 10: 328-333.
7. CID, V. J., DURAN, F. DEL REY, M. P. SNYDER, C. NOMBELA et al., 1995 Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol. Rev. 59: 345-386.
8. COHEN, P., 1989 The structure and regulation of protein phosphatases. Annu. Rev. Biochem. 58: 453-508.
9. COHEN, P. T. W., 1994 Nomenclature and chromosomal localization of human protein serine/threonine phosphatase genes. Adv. Prot. Phosphatases 8: 371-376.
10. COHEN, P., D. L. SCHELLING and M. J. R. STARK, 1989 Remarkable similarities between yeast and mammalian protein phosphatases. FEBS Letts. 250: 601-606.
11. COSTIGAN, C., S. GEHRUNG and M. SNYDER, 1992 A synthetic lethal screen identifies SLK1, a novel protein kinase homolog implicated in yeast cell morphogenesis and cell growth. Mol. Cell. Biol. 12: 1162-1178.
12. COSTIGAN, C., D. KOLODRUBETZ and M. SNYDER, 1994 NPH6A and NPH6B, which encode HMG1-like proteins, are candidates for downstream components of the the SLT2 mitogen-activated protein kinase pathway. Mol. Cell. Biol. 14: 2391-2403.
13. 1 cyclins CLN1 and CLN2 and a GAP-like protein have a role in bud formation. EMBO J. 12: 5277-5286.
14. DMOCHOWSKA, A., P. GOLIK and P. P. STEPIEN, 1995 The novel nuclear gene DSS-1 of Saccharomyces cerevisiae is necessary for mitochondrial biogenesis. Curr. Genet. 28: 108-112.
15. DOSEFF, A. I., and K. T. ARNDT, 1995 LAS1 is an essential nuclear protein involved in cell morphogenesis and cell surface growth. Genetics 141: 857-871.
16. DRGONOVA, J., T. DRGON, K. TANAKA, R. KOLIAR, G.-C. CHEN et al., 1996 Rho1p, a yeast protein at the interface between cell polarization and morphogenesis. Science 272: 277-279.
17. EGLOFF, M. P., P. T. W. COHEN, P. REINEMER and D. BARFORD, 1995 Crystal structure of the catalytic subunit of human protein phosphatase I and its complex with tungstate. J. Mol. Biol. 254: 942-959.
18. ERREDE, B., and D. E. LEVIN, 1993 A conserved kinase cascade for MAP kinase activation in yeast. Curr. Biol. 5: 254-260.
19. EVANS, D. R. H., C. RASMUSSEN, P. J. HANIC-JOYCE, G. C. JOHNSTON, R. A. SINGER et al., 1995 Mutational analysis of the Prt1 protein subunit of yeast translation initiation factor 3. Mol. Cell. Biol. 15: 4525-4535.
20. FERNANDEZ-SARABIA, M. J., A. SUTTON, T. ZHONG and K. T. ARNDT, 1992 SIT4 protein phosphatase is required for the normal accumulation of SWI4, CLN1, CLN2 and HCS26 RNAs during late G1. Genes Dev. 6: 2417-2428.
21. GIETZ, R. D., and A. SUGINO, 1988 New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene 74: 527-534.
22. HEALY, A. M., S. ZOLNIEROWICZ, A. E. STAPLETON, M. GOEBL, A. A. DEPAOLI-ROACH et al., 1991 CDC55, a Saccharomyces cerevisiae gene involved in cellular morphogenesis: identification, characterization and homology to the B subunit of mammalian type 2A protein phosphatase. Mol. Cell. Biol. 11: 5767-5780.
23. HOFFMANN, R., S. JUNG, M. EHRMANN and H. W. HOFER, 1994 The Saccharomyces cerevisiae gene PPH3 encodes a protein phosphatase with properties different from PPX, PP1 and PP2A. Yeast 10: 567-578.
24. KAISER, C., S. MICHAELIS and A. MITCHELL, 1994 Methods in Yeast Genetics, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
25. KAMADA, Y., H. QADOTA, C. P. PYTHON, Y. ANRAKU, Y. OHYA et al., 1996 Activation of yeast protein kinase C by Rho1 GTPase. J. Biol. Chem. 271: 9193-9196.
26. KARPOVA, T. S., M. M. LEPETIT and J. A. COOPER, 1993 Mutations that enhance the cap2 null mutant phenotype in Saccharomyces cerevisiae affect the actin cytoskeleton, morphogenesis and pattern of growth. Genetics 135: 693-709.
27. KILMARTIN, J., and A. E. M. ADAMS, 1984 Structural rearrangements of tubulin and actin during the cell cycle of the yeast Saccharomyces cerevisiae. J. Cell Biol. 98: 922-933.
28. KIM, Y.-J., L. FRANCISCO, G.-C. CHEN, E. MARCOTTE and C. S. M. CHAN, 1994 Control of cellular morphogenesis by Ip12/Bem2 GTPase-activating protein: possible role of protein phosphorylation. J. Cell Biol. 127: 1381-1394.
29. KINOSHITA, N., H. OHKURA and M. YANAGIDA, 1990 Distinct, essential roles of type 1 and 2A protein phosphatases in the control of the fission yeast cell division cycle. Cell 63: 405-415.
30. + gene encodes a 110-kDa essential protein implicated in mitotic control. Mol. Cell. Biol. 11: 5839-5847.
31. KINOSHITA, N., H. YAMANO, H. NIWA, T. YOSHIDA and M. YANAGIDA, 1993 Negative regulation of mitosis by the fission yeast protein phosphatase ppa2. Genes Dev. 7: 1059-1071.
32. KINOSHITA, K, T. NEMOTO, K. NABESHIMA, H. KONDOH, H. NIWA et al., 1996 The regulatory subunits of fission yeast protein phosphatase 2A (PP2A) affect cell morphogenesis, cell wall synthesis and cytokinesis. Genes Cells 1: 29-45.
33. LEE, K. S., K. IRIE, Y. GOTOH, Y. WATANABE, H. ARAKI et al., 1993 A yeast mitogen-activated protein kinase homolog (Mpk1) mediates signalling by protein kinase C. Mol. Cell. Biol. 13: 3067-3075.
34. LEE, T. H., M. J. SOLOMON, M. C. MUMBY and M. W. KIRSCHNER, 1991 INH, a negative regulator of MPF, is a form of protein phosphatase 2A. Cell 64: 415-423.
35. LEE, T. H., C. TURCK and M. W. KIRSCHNER, 1994 Inhibition of cdc2 activation by INH/PP2A. Mol. Cell. Biol. 5: 323-338.
36. LEUNG, D. W., E. CHEN and D. V. GOEDDEL, 1989 A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction. Technique A. J. Cell. Mol. Biol. 1: 11-15.
37. LEW D. J., and S. I. REED, 1993 Morphogenesis in the yeast cell cycle: regulation by Cdc28 and cyclins. J. Cell Biol. 120: 1305-1320.
38. LIN, F. C., and K. ARNDT, 1995 The role of Saccharomyces cerevisiae type 2A phosphatase in the actin cytoskeleton and in entry into mitosis. EMBO J. 14: 2745-2759.
39. MAYER-JAEKEL, R. E., H. OHKURA, R. GOMES, C. E. SUNKEL, S. BAUMGARTNER et al., 1993 The 55 kd regulatory subunit of Drosophila protein phosphatase 2A is required for anaphase. Cell 72: 621-633.
40. MUHLRAD, D., R. HUNTER and R. PARKER, 1992 A rapid method for localized mutagenesis of yeast genes. Yeast 8: 79-82.
41. NASMYTH, K., and L. DIRICK, 1991 The role of SWI4 and SWI6 in the activity of G1 cyclins in yeast. Cell 66: 995-1013.
42. NONAKA, H., K. TANAKA, H. HIRANO, T. FUJIWARA, H. KOHNO et al., 1995 A downstream target of RHOI small GTP-binding protein is PKC1, a homolog of protein kinase C, which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae. EMBO J. 14: 5931-5938.
43. PALMER, R. E., D. S. SULLIVAN, T. HLIFFAKER and D. KOSHIAND, 1992 Role of astral microtubules and actin in spindle orientation and migration in the budding yeast Saccharomyces cerevisiae. J. Cell Biol. 119: 583-593.
44. PETERSON, J., Y. ZHENG, L. BENDER, A. MYERS, R. CERIONE et al., 1994 Interactions between the bud emergence proteins Bem1p and Bem2p and Rho-type GTPases in yeast. J. Cell Biol. 127: 1395-1406.
45. QADOTA, H., C. P. PYTHON, S. B. INOUE, M. ARISAWA, Y. ANRAKU et al., 1996 Identification of yeast Rho1p GTPase as a regulatory subunit of 1,3-β-glucan synthase. Science 272: 279-281.
46. RONNE, H., M. CARLBERG, G.-Z. Hu and J. O. NEHLIN, 1991 Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis. Mol. Cell. Biol. 11: 4876-4884.
47. SAMBROOK, J., E. F. FRITSCH and T. MANIATIS, 1989 Molecular Cloning: A Laboratory Manual, Ed. 2. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
48. SCHIESTL, R. H., and R. D. GIETZ, 1989 High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr. Genet. 16: 339-346.
49. SIKORSKI, R. S., and P. HIETER, 1989 A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
50. SNEDDON, A. A., P. T. W. COHEN and M. J. R. STARK, 1990 Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes. EMBO J. 9: 4339-4346.
51. SONTAG, E., V. NUNBHAKDI-CRAIG, G. S. BLOOM and M. C. MUMBY, 1995 A novel pool of protein phosphatase 2A is associated with microtubules and is regulated during the cell cycle. J. Cell Biol. 128: 1131-1144.
52. 1 for progression into S phase. Mol. Cell. Biol. 11: 2133-2148.
53. TURCQ, B., K. F. DOBINSON, N. SERIZAWA and A. M. LAMBOWITZ, 1992 A protein required for RNA processing and splicing in Neurospora mitochondria is related to gene products involved in cell cycle protein phosphatase functions. Proc. Natl. Acad. Sci. USA 89: 1676-1680.
54. URSIC, D., J. C. SEDBROOK, K. L. HIMMEL and M. R. CULBERTSON, 1994 The essential yeast Tcp1 protein affects actin and microtubules. Mol. Biol. Cell 5: 1065-1080.
55. VAN ZYL, W., W. HUANG, A. A. SNEDDON, M. STARK, S. CAMIER et al., 1992 Inactivation of the protein phosphatase 2A regulatory subunit A results in morphological and transcriptional defects in Saccharomyces cerevisiae. Mol. Cell. Biol. 12: 4946-4959.
56. WANG, T., and A. BRETSCHER, 1995 The rho-GAP encoded by BEM2 regulates cytoskeletal structure in budding yeast. Mol. Biol. Cell 6: 1011-1024.
57. WASSERMAN, D. A., N. M. SOLOMON, H. C. CHANG, F. D. KARIM, M. THERRIEN et al., 1996 Protein phosphatase 2A positively and negatively regulates Ras1-mediated photoreceptor development in Drosophila. Genes Dev. 10: 272-278.
58. WATANABE, Y., K. IRIE and K. MATSUMOTO, 1995 Yeast RLM1 encodes a serum response factor-like protein that may function downstream of the Mpk1 (S1t2) mitogen-activated protein kinase pathway. Mol. Cell. Biol. 15: 5740-5749.
59. WILSON, R. B., A. A. BRENNER, T. B. WHITE, M. J. ENGLER, J. P. GAUGHRAN et al., 1991 The Saccharomyces cerevisiae SRK1 gene, a suppressor of bcy1 and ins1, may be involved in protein phosphatase function. Mol. Cell. Biol. 11: 3369-3373.
60. ZARZOV, P., C. MAZZONI and C. MANN, 1996 The SLT2(MPK1) MAP kinase is activated during periods of polarized cell growth in yeast. EMBO J. 15: 83-91.