One-step duplex reverse transcription-polymerase chain reaction for quantitative assessment of RNA degradation

Analytical Biochemistry

Volumn 295, Issue 1, 2001, Pages 113-116

  Sugita, Michio ^a   Haney, Jerry L ^a   Gemmill, Robert M ^a   Franklin, Wilbur A ^a  

^a UNIVERSITY OF COLORADO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

QUANTITATIVE ASSESSMENTS; REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION;

POLYMERASE CHAIN REACTION;

ACCURACY; ANALYTIC METHOD; ARTICLE; GENE AMPLIFICATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA DEGRADATION;

EID: 0035423837     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5216     Document Type: Article

References (8)

1. Isolation and crystallization of enolase
2. Validity of nucleic acid purities monitored by 260nm/280nm absorbance ratios
3. The expression of cytokine and cytokine receptor genes in long-term bone marrow culture in congenital and acquired bone marrow hypoplasias
4. Control genes for reverse-transcription-polymerase chain reaction: A comparison of beta actin and glyceraldehyde phosphate dehydrogenase
5. Multiplex PCR for quality control of template RNA/cDNA in RT-PCR assays
6. Quantitative determination of gene expression by competitive reverse transcription-polymerase chain reaction in degraded RNA samples
7. Assessment of RNA quality by semi-quantitative RT-PCR of multiple regions of a long ubiquitous mRNA
8. Preferential expression of novel MUC1 tumor antigen isoforms in human epithelial tumors and their tumor-potentiating function