Multiplex PCR for quality control of template RNA/cDNA in RT-PCR assays

Leukemia

Volumn 12, Issue 12, 1998, Pages 1984-1993

  Watzinger, F ^a   Lion, T ^a  

^a CHILDREN'S CANCER RESEARCH INSTITUTE   (Austria)

Author keywords

ABL; BCR; Beta 2 microglobulin; Multiplex PCR; Porphobilinogen deaminase; RT PCR

Indexed keywords

ACTIN; DNA; MESSENGER RNA;

ARTICLE; GENETICS; LABORATORY DIAGNOSIS; METHODOLOGY; MINIMAL RESIDUAL DISEASE; QUALITY CONTROL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STANDARD;

ACTINS; DNA; NEOPLASM, RESIDUAL; QUALITY CONTROL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPECIMEN HANDLING;

EID: 0032420845     PISSN: 08876924     EISSN: 14765551     Source Type: Journal    
DOI: 10.1038/sj.leu.2401217     Document Type: Article

References (11)

1. Nagel S, Schmidt M, Thiede C, Huhn D, Neubauer A. Quantification of Bcr-Abl transcripts in chronic myelogenous leukemia (CML) using standardized, internally controlled, competitive differential PCR (CD-PCR). Nucleic Acids Res 1996; 24: 4102-4103.
2. Kang YK, Zhan Z, Regis J, Alvarez M, Robey R, Meadows B, Dickstein B, Lee JS, Otsuki T, Stetler Stevenson M et al. Expression of mdr-1 in refractory lymphoma: quantitation by polymerase chain reaction and validation of the assay (published erratum appears in Blood 1995; 86: 4710). Blood 1995; 86: 1515-1524.
3. Hochhaus A, Lin F, Reiter A, Skladny H, Mason PJ, van Rhee F, Shepherd PC, Allan NC, Hehlmann R, Goldman JM, Cross NC. Quantification of residual disease in chronic myelogenous leukemia patients on interferon-alpha therapy by competitive polymerase chain reaction. Blood 1996; 87: 1549-1555.
4. van Rhee F, Marks DI, Lin F, Szydlo RM, Hochhaus A, Treleaven J, Delord C, Cross NC, Goldman JM. Quantification of residual disease in Philadelphia-positive acute lymphoblastic leukemia: comparison of blood and bone marrow. Leukemia 1995; 9: 329-335.
5. Kidd V, Lion T. Debate round-table. Appropriate controls for RTPCR. Leukemia 1997; 11: 871-881.
6. Tolan DR, Niclas J, Bruce BD, Lebo RV. Evolutionary implications of the human aldolase-A, -B, -C, and -pseudogene chromosome locations. Am J Hum Genet 1987; 41: 907-924.
7. Wells D, Bains W. Characterization of an unusual human histone H3.3 pseudogene. DNA Seq 1991; 2: 125-127.
8. Tso JY, Sun XH, Kao TH, Reece KS, Wu R. Isolation and characterization of rat and human glyceraldehyde-3-phosphate dehydrogenase cDNAs: genomic complexity and molecular evolution of the gene. Nucleic Acids Res 1985; 13: 2485-2502.
9. Moos M, Gallwitz D. Structure of two human beta-actin-related processed genes one of which is located next to a simple repetitive sequence. EMBO J 1983; 2: 757-761.
10. Masters JN, Yang JK, Cellini A, Attardi G. A human dihydrofolate reductase pseudogene and its relationship to the multiple forms of specific messenger RNA. J Mol Biol 1983; 167: 23-26.
11. Lion T. Appropriate controls for RT-PCR. Leukemia 1996; 10: 1843.