Insights into the Specificity and Function of (Allo)antigen-reactive T Cells

American Journal of Transplantation

Volumn 1, Issue 2, 2001, Pages 109-114

  Volk, Hans Dieter ^a   Kern, Florian ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

Antigen reactive T cells; ELISPOT; Flow cytometry; Intracellular cytokine staining

Indexed keywords

ALLOANTIGEN; AUTOANTIGEN;

ANTIBODY SPECIFICITY; HUMAN; IMMUNOLOGY; MAJOR HISTOCOMPATIBILITY COMPLEX; REVIEW; T LYMPHOCYTE;

ANTIBODY SPECIFICITY; AUTOANTIGENS; HUMANS; ISOANTIGENS; MAJOR HISTOCOMPATIBILITY COMPLEX; T-LYMPHOCYTES;

EID: 0035401094     PISSN: 16006135     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1600-6143.2001.10203.x     Document Type: Article

References (14)

1. Givan AL, Fisher JU, Waugh M, Emstoff MS, Wallace PK. A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens. J Immunol Methods 1999; 230: 99-112.
2. Noorchashm H, Lieu YK, Rostami SY et al. A direct method for the calculation of alloreactive CD4+ T cell precursor frequency. Transplantation 1999; 67: 1281-1284.
3. Suchin EJ, Langmuir PB, Palmer E et al. Quantifying the frequency of alloreactive T cells in vivo: new answers to an old question. J Immunol 2001; 166: 973-981.
4. Waldrop SL, Pitcher CJ, Peterson DM, Maino VC, Picker LJ. Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency. J Clin Invest 1997; 99: 1739-1750.
5. Suni MA, Picker LJ, Maino VC. Detection of antigen-specific T cell cytokine expression in whole blood by flow cytometry. J Immunol Methods 1998; 212: 89-98.
6. Kern F, Surel IP, Brock C et al. T-cell epitope mapping by flow cytometry. Nat Med 1998; 4: 975-978.
7. Kern F, Faulhaber N, Frömmel C et al. Analysis of CD8 T cell reactivity to cytomegalovirus using protein-spanning pools of overlapping pentapeptides. Eur J Immunol 2000; 30: 1676-1682.
8. Heeger PS, Greenspan NS, Kuhlenschmidt S et al. Pretransplant frequency of donor-specific, IFN-gamma producing lymphocytes is a manifestation of immunologic memory and correlates with the risk of posttransplant rejection episodes. J Immunol 1999; 163: 2267-2275.
9. Valujskikh A, Heeger PS. A closer look at cytokine secreting T lymphocytes. Graft 2000; 3: 250-259.
10. Brosterhus H, Brings S, Leyendeckers H et al. Enrichment and detection of live antigen-specific CD4+ and CD8+ T cells based on cytokine secretion. Eur J Immunol 1999; 29: 4053-4059.
11. Scheffold A, Assenmacher M, Reners-Schramm L, Lauster R, Radbruch A. High-sensitivity immunofluorescence for detection of the pro-and anti-inflammatory cytokines gamma Interferon and interleukin-10 on the surface of cytokine-secreting cells. Nat Med 2000; 6: 107-110.
12. Crawford F, Kozono H, White J, Marrack P, Kappler J. Detection of antigen-specific T cells with multivalent soluble class II MHC covalent peptide complexes. Immunity 1998; 8: 675-682.
13. Ogg GS, McMichael AJ. HLA-peptide tetrameric complexes. Curr Opin Immunol 1998; 10: 393-396.
14. Obst R, Netuschil N, Klopfer K, Stevanovic S, Rammense HG. The role of peptides in T cell alloreactivity is determined by self-major histocompatibility complex molecules. J Exp Med 2000; 6: 805-812.