Flow cytometric DNA-quantification of three-color immunophenotyped cells for subpopulation specific determination of aneuploidy and proliferation

Journal of Immunological Methods

Volumn 253, Issue 1-2, 2001, Pages 145-152

  Oelschlaegel, Uta ^a   Freund, Daniel ^a   Range, Ursula ^a   Ehninger, Gerhard ^a   Nowak, Ralf ^a  

^a UNIVERSITY HOSPITAL CARL GUSTAV CARUS   (Germany)

Author keywords

Aneuploidy; DNA quantification; Flow cytometry; Multiparameter immunophenotyping

Indexed keywords

ANTIGEN; DNA;

ANEUPLOIDY; ANTIGEN EXPRESSION; ARTICLE; B LYMPHOCYTE; CANCER CELL CULTURE; CHEMOTHERAPY; DNA CONTENT; FLOW CYTOMETRY; HEMATOPOIESIS; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LYMPHOCYTE PROLIFERATION; LYMPHOCYTE SUBPOPULATION; NORMAL HUMAN; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE GENETICS; T LYMPHOCYTE; TUMOR CELL;

ANEUPLOIDY; CELL CYCLE; CELLS, CULTURED; COLOR; DNA, NEOPLASM; FLOW CYTOMETRY; FLUORESCENT DYES; HUMANS; IMMUNOPHENOTYPING; INDOLES; LYMPHOCYTE ACTIVATION; MULTIPLE MYELOMA; T-LYMPHOCYTE SUBSETS; TUMOR CELLS, CULTURED;

EID: 0035400267     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(01)00389-1     Document Type: Article

References (12)

1. High-sensitive immunophenotyping and DNA ploidy studies for the investigation of minimal residual disease in multiple myeloma
2. Simultaneous measurement of DNA content and cell-surface immunofluorescence of human bone marrow cells using a single laser flow cytometer
3. Improved single laser measurement of two cellular antigens and DNA-ploidy by the combined use of propidium iodide and TO-PRO-3 iodide
4. Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer
5. Simultaneous quantification of c-myc oncoprotein, total cellular protein, and DNA content using multiparameter flow cytometry
6. Three-color versus four-color multiparameter cell cycle analyses of primary acute myeloid leukemia samples
7. Simultaneous analysis of DNA content and surface antigens in human bone marrow
8. Sensitivity of combined DNS/immunophenotype flow cytometry for the detection of low levels of aneuploid lymphoblastic leukemia cells in bone marrow
9. Relations between IL3-induced proliferation and in vitro cytokine secretion of bone marrow cells from AML patients
10. Flow cytometric DNA quantification in immunophenotyped cells as a sensitive method for determination of aneuploid multiple myeloma cells in peripheral blood stem cell harvests and bone marrow after therapy
11. A new method for the analysis of plasma cell DNA content in Multiple Myeloma samples using a CD38/propidium iodide double staining technique
12. Measurement of lymphocyte subset proliferation by three-color immunofluorescence and DNA flow cytometry