Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer

Cytometry

Volumn 39, Issue 2, 2000, Pages 96-107

  Corver, Willem E ^a   Koopman, Louise A ^a   Van Der Aa, Jan ^b   Regensburg, Mira ^c   Fleuren, Gert Jan ^a   Cornelisse, Cees J ^a  

^a LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^b Immunocytometry Systems   (Belgium)

^c LEIDEN CYTOLOGY AND PATHOLOGY LABORATORY   (Netherlands)

Author keywords

Cervical cancer; DNA ploidy; Ep CAM; Flow cytometry; Keratin; Multiparameter; Tumor heterogeneity; Vimentin

Indexed keywords

ALLOPHYCOCYANIN; CELL ADHESION MOLECULE; DNA; KERATIN; KERATIN ANTIBODY; PROPIDIUM IODIDE; VIMENTIN;

ARTICLE; CELL HETEROGENEITY; COLOR; CONTROLLED STUDY; FLOW CYTOMETRY; HUMAN; HUMAN CELL; LASER FLOWMETER; PHENOTYPE; PLOIDY; PRIORITY JOURNAL; STAINING; UTERINE CERVIX CANCER;

ANTIBODIES, MONOCLONAL; ANTIGENS, NEOPLASM; CARBOCYANINES; CELL ADHESION MOLECULES; DNA; FEMALE; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; HUMANS; KERATINS; LYMPHOCYTES; PHENOTYPE; PHYCOCYANIN; PROPIDIUM; TUMOR CELLS, CULTURED; UTERINE CERVICAL NEOPLASMS;

EID: 0033974317     PISSN: 01964763     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0320(20000201)39:2<96::AID-CYTO2>3.0.CO;2-X     Document Type: Article

References (31)

1. Corver WE, Cornelisse CJ, Fleuren GJ. Simultaneous measurement of two cellular antigens and DNA using fluorescein-isothiocyanate, R-phycoerythrin, and propidium iodide on a standard FACScan. Cytometry 1994;15:117-128.
2. Corver WE, Bonsing BA, Abeln ECA, Vlak-Theil PM, Cornelisse CJ, Fleuren GJ. One tube triple staining method for the flow cytometric analysis of DNA ploidy and phenotypic heterogeneity of human solid tumors using single laser excitation. Cytometry 1996;25:358-366.
3. Brockhoff G, Endl E, Minuth W, Hofstädter F, Knüchel R. Options of flow cytometric three-colour DNA measurements to quantitate EGFR in subpopulations of human bladder cancer. Anal Cell Pathol 1996; 11:55-70.
4. Li BDL, Harlow SP, Budnick RM, Sheedy DL, Stewart CC. Detection of HER-2/neu oncogene amplification in flow cytometry-sorted breast ductal cells by competitive polymerase chain reaction. Cancer 1994; 73:2771-2778.
5. Abeln ECA, Corver WE, Kuipers-Dijkshoorn NJ, Fleuren GJ, Cornelisse CJ. Molecular genetic analysis of flow-sorted ovarian tumour cells: improved detection of loss of heterozygosity. Br J Cancer 1994;70: 255-262.
6. Bonsing BA, Corver WE, Gorsira MCB, van Vliet M, Oud PS, Cornelisse CJ, Fleuren GJ. Specificity of seven monoclonal antibodies against p53 evaluated with Western blotting, immunohistochemistry, confocal laser scanning microscopy and flow cytometry. Cytometry 1997;28: 11-24.
7. Corver WE, Fleuren GJ, Cornelisse CJ. Improved single laser measurement of two cellular antigens and DNA-ploidy by the combined use of propidium iodide and TO-PRO-3 iodide. Cytometry 1997;28:329-336.
8. Hirons GT, Fawcett JJ, Crissman HA. TOTO and YOYO: new very bright fluorochromes for DNA content analyses by flow cytometry. Cytometry 1994;15:129-140.
9. Doornbos RMP, De Grooth BG, Kraan YM, Van Der Poel CJ, Greve J. Visible diode lasers can be used for flow cytometric immunofluorescence and DNA analysis. Cytometry 1994;15:267-271.
10. Glade CP, Botermans RJG, Van Erp PEJ, Van de Kerkhof PCM. The dynamics of the response of normal skin to single and multiple epicutaneous leukotriene B4 applications analysed by by three-colour flow cytometry. Acta Derm Venereol [Stockh] 1995;75:437-441.
11. Glade CP, Van Erp PEJ, Van de Kerkhof PCM. Epidermal cell DNA content and intermediate filaments keratin 10 and vimentin after treatment of psoriasis with calcipotriol cream once daily, twice daily and in combination with clobetasone 17-butyrate cream or betamethasone 17-valerate cream: a comparative flow cytometric study. Br J Dermatol 1996;135:379-384.
12. Smedts F, Ramaekers F, Troyanovsky S, Pruszczynski M, Link M, Lane B, Leigh I, Schijf C, Vooijs P. Keratin expression in cervical cancer. Am J Pathol 1992;141:497-511.
13. Litvinov SV, Velders MP, Bakker HAM, Fleuren GJ, Warnaar SO. Ep-CAM: a human epithelial antigen is a homophilic cell-cell adhesion molecule. J Cell Biol 1994;125:437-446.
14. Moldenhauer G, Momburg F, Möller P, Schwartz R, Hämmerling GJ. Epithelium-specific surface glycoprotein of Mr 34,000 is a widely distributed human carcinoma marker. Br J Cancer 1987;56:714-721.
15. Litvinov SV, Van Driel W, Van Rhijn CM, Bakker HAM, Van Krieken H, Fleuren GJ, Warnaar SO. Expression of Ep-CAM in cervical squamous epithclia correlates with an increased proliferation and the disappearance of markers for terminal differentiation. Am J Pathol 1996;148: 865-875.
16. Corver WE, Cornelisse CJ, Hermans J, Fleuren GJ. Limited loss of nine tumor-associated surface antigenic determinants after tryptic cell dissociation. Cytometry 1995;19:267-272.
17. Ensley JF, Maciorowski Z, Pietraszkiewicz H, Klemic G, KuKuruga M, Sapareto S, Corbett T, Crissman J. Solid tumor preparation for flow cytometry using a standard murinc model. Cytometry 1987;8:479-487.
18. Van Muijen GNP, Ruiter DJ, Warnaar SO. Coexpression of intermediate filament polypeptides in human fetal and adult tissues. Lab Invest 1987;57:359-369.
19. Van Muijen GNP, Warnaar SO, Ponec M. Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis. Exp Cell Res 1987;171:331-345.
20. Kimmig R, Kapsner T, Spelsberg H, Untch M, Hepp H. DNA cell-cycle analysis of cervical cancer by flow cytometry using simultaneous cytokeratin labelling for identification of tumour cells. J Cancer Res Clin Oncol 1995;121:107-114.
21. Zarbo RJ, Visscher D, Crissman JD. Two-color multiparametric method for flow cytometric DNA analysis of carcinomas using staining for cytokeratin and leukocyte-common antigen. Anal Quant Cytol Histol 1989;11:391-402.
22. Van Muijen GNP, Ruiter DJ, Ponec M, Huiskens-van der Mey C, Warnaar SO. Monoclonal antibodies with different specificities against cytokeratin. Am J Pathol 1984;114:9-17.
23. Ulich TR, Liao SY, Layfield L, Romansky S, Cheng L, Lewin KJ. Endocrine and tumor differentiation markers in poorly differentiated small-cell carcinoids of the cervix and vagina. Arch Pathol Lab Med 1986;110:1054-1057.
24. Prieto VG, Lugo J, McNutt NS. Intermediate-and low-molecular-weight keratin detection with the monoclonal antibody MNF116. An immunohistochemical study on 232 paraffin-embedded cutaneous lesions. J Cutan Pathol 1996;23:234-241.
25. Van Muijen GNP, Ruiter DJ, Franke WW, Achtstätter T, Haasnoot WHB, Ponec M, Warnaar SO. Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res 1986;162:97-113.
26. Wauters CC, Smedts F, Gerrits LG, Bosman FT, Ramaekers FC. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol 1995;26:852-855.
27. Schaafsma HE, Ramaekers FC, van Muijen GN, Ooms EC, Ruiter DJ. Distribution of cytokeratin polypeptides in epithelia of the adult human urinary tract. Histochemistry 1989;91:151-159.
28. Ivanyi D, Ansink A, Groeneveld E, Hageman PC, Mooi WJ, Heintz AP. New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. J Pathol 1989;159:7-12.
29. Levy R, Czernobilsky B, Geiger B. Subtyping of epithelial cells of normal and metaplastic human uterine cervix, using polypeptide-specific cytokeratin antibodies. Differentiation 1988;39:185-196.
30. Broers JL, Ramaekers FC, Rot MK, Oostendorp T, Huysmans A, van Muijen GN, Wagenaar SS, Vooijs GP. Cytokeratins in different types of human lung cancer as monitored by chain-specific monoclonal antibodies. Cancer Res 1988;48:3221-3229.
31. Perkins W, Campbell I, Leigh IM, MacKie RM. Keratin expression in normal skin and epidermal neoplasms demonstrated by a panel of monoclonal antibodies. J Cutan Pathol 1992;19:476-482.