Active-site mutations impairing the catalytic function of the catalytic subunit of human protein phosphatase 2A permit baculovirus-mediated overexpression in insect cells

Biochemical Journal

Volumn 357, Issue 1, 2001, Pages 225-232

  Myles, T ^a   Schmidt, K ^a   Evans, D R H ^a   Cron, P ^a   Hemmings, B A ^a  

^a FRIEDRICH MIESCHER INSTITUTE FOR BIOMEDICAL RESEARCH   (Switzerland)

Author keywords

Kinetics; Mutagenesis; PP2A; Recombinant protein; Yeast

Indexed keywords

CATALYST ACTIVITY; CELL CULTURE; ENZYME KINETICS; MOLECULAR STRUCTURE; MUTAGENESIS; YEAST;

MUTATIONS;

BIOCHEMISTRY;

PHOSPHOPROTEIN PHOSPHATASE;

AMINO ACID SUBSTITUTION; ANIMAL CELL; ARTICLE; AUTOREGULATION; BACULOVIRUS; CATALYSIS; DEPHOSPHORYLATION; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; INSECT; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FAMILY; SITE DIRECTED MUTAGENESIS; VIRUS EXPRESSION;

AMINO ACID SUBSTITUTION; ANIMALS; ASPARTIC ACID; BACULOVIRIDAE; BINDING SITES; CATALYSIS; CELL LINE; HISTIDINE; HUMANS; KINETICS; MUTAGENESIS, SITE-DIRECTED; PHOSPHOPROTEIN PHOSPHATASE; PROTEIN SUBUNITS; RECOMBINANT PROTEINS; SPODOPTERA; TRANSFECTION;

ANIMALIA; INSECTA; SACCHAROMYCETALES; UNIDENTIFIED BACULOVIRUS;

EID: 0035395687     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/0264-6021:3570225     Document Type: Article

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