Intact microglia are cultured and non-invasively harvested without pathological activation using a novel cultured cell recovery method

Biomaterials

Volumn 22, Issue 11, 2001, Pages 1213-1223

  Nakajima, Kazuyuki ^a,b   Honda, Shizuyo ^a   Nakamura, Yasuko ^a   López Redondo, Fernando ^a   Kohsaka, Shinichi ^a   Yamato, Masayuki ^c   Kikuchi, Akihiko ^c   Okano, Teruo ^c  

^a NATIONAL INSTITUTE OF NEUROSCIENCE   (Japan)

^b SOKA UNIVERSITY   (Japan)

^c TOKYO WOMEN'S MEDICAL UNIVERSITY   (Japan)

Author keywords

Cell culture; Microglia; Poly(N isopropylacrylamide); Temperature responsive; Tissue engineering

Indexed keywords

HYDRATION; HYDROPHILICITY; NEUROLOGY; ORGANIC POLYMERS; TRANSPLANTATION (SURGICAL);

MICROGLIA;

CELL CULTURE;

BRAIN DERIVED NEUROTROPHIC FACTOR; EDETIC ACID; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; NEUROTROPHIC FACTOR; POLY(N ISOPROPYLACRYLAMIDE); POLYMER; TRYPSIN; TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; BIOENGINEERING; CELL CULTURE; CELL ISOLATION; CELL PROLIFERATION; CELL STRUCTURE; CELL TRANSPLANTATION; CENTRAL NERVOUS SYSTEM; CONTROLLED STUDY; HYDRATION; HYDROPHILICITY; IMMUNOBLOTTING; IMMUNOCYTOCHEMISTRY; MICROGLIA; NEWBORN; NONHUMAN; PATHOLOGY; PRIORITY JOURNAL; RAT; SURFACE PROPERTY; TECHNIQUE; TEMPERATURE; TISSUE ENGINEERING;

ANIMALS; ANIMALS, NEWBORN; BRAIN-DERIVED NEUROTROPHIC FACTOR; CELL CULTURE TECHNIQUES; CULTURE TECHNIQUES; IMMUNOHISTOCHEMISTRY; MICROGLIA; NF-KAPPA B; RATS; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0035371956     PISSN: 01429612     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0142-9612(00)00270-2     Document Type: Article

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