Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium

Neuroscience Research

Volumn 25, Issue 3, 1996, Pages 203-208

  Amano, Hiro ^a   Kurosaka, Reiko ^a   Ema, Makoto ^a   Ogawa, Yoshiyuki ^a  

^a NATIONAL INSTITUTE OF HEALTH SCIENCES   (Japan)

Author keywords

C6 glioma cells; Cell growth; Proliferation; Serum free medium; Trypsin

Indexed keywords

ANTIMITOTIC AGENT; GROWTH FACTOR; SERINE PROTEINASE; SOYBEAN TRYPSIN INHIBITOR; TRYPSIN;

ANIMAL CELL; ARTICLE; CELL ADHESION; CELL DENSITY; CELL GROWTH; CELL PROLIFERATION; CELL SUSPENSION; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; GLIOMA CELL; NONHUMAN; PRIORITY JOURNAL; RAT;

EID: 0030200196     PISSN: 01680102     EISSN: None     Source Type: Journal    
DOI: 10.1016/0168-0102(96)01007-3     Document Type: Article

References (29)

1. Benda, P., Lightbody, J., Sato, G., Levine, L. and Sweet, W. (1968) Differentiated rat glial cell strain in tissue culture. Science, 161: 370-371.
2. Bennett, K., McGinnis, J.F. and de Vellis, J. (1977) Reversible inhibition of the hydrocortisone induction of glycerol phosphate dehydrogenase by cytochalasin B in rat glial C6 cells. J. Cell. Physiol., 93: 247-260.
3. Bradshaw, S.L., Naus, C.C.G., Zhu, D., Kidder, G.M., D'Ercole, A.J. and Han, V.K.M. (1993) Alterations in the synthesis of insulin-like growth factor binding proteins and insulin-like growth factors in rat C6 glioma cells transfected with a gap junction connexin43 cDNA. Regul. Pept., 48: 99-112.
4. Coyle, D.E. (1995) Adaptation of C6 glioma cells to serum-free conditions leads to the expression of a mixed astrocyte-oligoden-drocyte phenotype and increased production of neurite-promoting activity. J. Neurosci. Res., 41: 374-385.
5. Donta, S.T. (1973) The growth of functional rat glial cells in a serumless medium. Exp. Cell Res., 82: 119-124.
6. Fan, K. and Uzman, B.G. (1977) Rat glioma cells (C6) cultured in serum-free defined medium. Exp. Cell Res., 106: 397-401.
7. Fischer, I., Nolan, C.E. and Shea, T.B. (1987) Effects of retinoic acid on expression of the transformed phenotype in C6 glioma cells. Life Sci., 41: 463-470.
8. Hayashi, Y., Kashiwagi, K., Ohta, J., Nakajima, M., Kawashima, T. and Yoshikawa, K. (1994) Alzheimer amyloid protein precursor enhances proliferation of neural stem cells from fetal rat brain. Biochem. Biophys. Res. Commun., 205: 936-943.
9. Herbert, J.M., Lamarche, I., Prabonnaud, V., Dol, F. and Gauthier, T. (1994) Tissue-type plasminogen activator is a potent mitogen for human aortic smooth muscle cells. J. Biol. Chem., 269: 3076-3080.
10. Kiess, W., Lee, L., Graham, D.E., Greenstein, L., Tseng, L.Y.-H., Rechler, M.M. and Nissley, S.P. (1989) Rat C6 glial cells synthesize insulin-like growth factor I (IGF-I) and express IGF-I receptors and IGF-II/mannose 6-phosphate receptors. Endocrinology, 124: 1727-1736.
11. Kumar, S., Weingarten, D.P., Callahan, J.W. and de Vellis, J. (1984) Regulation of mRNAs for three enzymes in the glial cell model C6 cell line. J. Neurochem., 43: 1455-1463.
12. Loret, C., Sensenbrenner, M. and Labourdette, G. (1989) Differential phenotypic expression induced in cultured rat astroblasts by acidic fibroblast growth factor, epidermal growth factor, and thrombin. J. Biol. Chem., 264: 8319-8327.
13. Lowe, Jr., W.L., Mayer, T., Karpen, C.W. and Lorentzen, L.R. (1992) Regulation of insulin-like growth factor I production in rat C6 glioma cells: possible role as an autocrine/paracrine growth factor. Endocrinology. 130: 2683-2691.
14. MacCumber, M.W., Ross, C.A. and Snyder, S.H. (1990) Endothelin in brain: receptors, mitogenesis, and biosynthesis in glial cells. Proc. Natl. Acad. Sci. USA. 87: 2359-2363.
15. Maltese, W.A. and Volpe, J.J. (1979) Induction of an oligodendroglial enzyme in C-6 glioma cells maintained at high density or in serum-free medium. J. Cell. Physiol., 101: 459-470.
16. Moonen, G., Grau-Wagemans, M.-P., Selak, I., Lefebvre, P.P., Rogister, B., Vassaili, J. and Belin, D. (1985) Plasminogen activator is a mitogen for astrocytes in developing cerebellum. Dev. Brain Res., 20: 41-48.
17. Okumura, N., Takimoto, K., Okada, M. and Nakagawa, H. (1989) C6 glioma cells produce basic fibroblast growth factor that can stimulate their own proliferation. J. Biochem., 106: 904-909.
18. Perraud, F., Besnard, F., Sensenbrenner, M. and Labourdette, G. (1987) Thrombin is a potent mitogen for rat astroblasts but not for oligodendroblasts and neuroblasts in primary culture. Int. J. Dev. Neurosci., 5: 181-188.
19. Pfeiffer, S.E., Herschman, H.R., Lightbody, J. and Sato, G. (1970) Synthesis by a clonal line of rat glial cells of a protein unique to the nervous system. J. Cell. Physiol., 75: 329-340.
20. Powell, P.P. and Klagsbrun, M. (1993) Regulation of basic fibroblast growth factor mRNA expression in rat C6 glioma cells. Exp. Cell Res., 209: 224-230.
21. Raju, T.R., Bignami, A. and Dahl, D. (1980) Glial fibrillary acidic protein in monolayer cultures of C-6 glioma cells: effect of aging and dibutyryl cyclic AMP. Brain Res., 200: 225-230.
22. Redekop, G.J. and Naus, C.C.G. (1995) Transfection with bFGF sense and antisense cDNA resulting in modification of malignant glioma growth. J. Neurosurg., 82: 83-90.
23. Rice, R.H., Means, G.E. and Brown, W.D. (1977) Stabilization of bovine trypsin by reductive methylation. Biochem. Biophys. Acta, 492 316-321.
24. Saksela, O. and Rifkin, D.B. (1990) Release of basic fibroblast growth factor-heparan sulfate complexes from endothelial cells by plasminogen activator-mediated proteolytic activity. J. Cell Biol., 110: 767-775.
25. Sedo, A., Rovero, P., Revoltella, R.P., Di Bartolo, V., Beffy, P. and Mizrahi, J. (1993) BQ-123 inhibits both endothelin 1 and endothelin 3 mediated C6 rat glioma cell proliferation suggesting an atypical endothelin receptor. J. Biol. Regul. Homeost. Agents, 7: 95-99.
26. Thornton, A.J., Bruzdzinski, C.J., Raper, S.E. and Gelehrter, T.D. (1994) Plasminogen activator inhibitor-1 is an immediate early-response gene in regenerating rat liver. Cancer Res., 54: 1337-1343.
27. Totani, L., Piccoli, A., Pellegrini, G. Di Santo, A. and Lorenzet, R. (1994) Polymorphonuclear leukocytes enhance release of growth factors by cultured endothelial cells. Arterioscler. Thromb., 14: 125-132.
28. Westermann, R., Hardung, M., Meyer, D.K., Ehrhard, P., Otten, U. and Unsicker, K. (1988) Neurotrophic factors released by C6 glioma cells. J. Neurochem., 50: 1747-1758.
29. Wolfe, R.A., Sato, G.H. and McClure, D.B. (1980) Continuous culture of rat C6 glioma in serum-free medium. J. Cell Biol., 87: 434-441.