A dual role of substrate S-adenosyl-L-methionine in the methylation reaction with bacteriophage T4 Dam DNA-[N6-adenine]-methyltransferase

Nucleic Acids Research

Volumn 29, Issue 11, 2001, Pages 2361-2369

  Malygin, Ernst G ^a   Evdokimov, Alexey A ^a   Zinoviev, Victor V ^a   Ovechkina, Lidiya G ^a   Lindstrom, William M ^b   Reich, Norbert O ^b   Schlagman, Samuel L ^c   Hattman, Stanley ^c  

^a STATE RESEARCH CENTER OF VIROLOGY AND BIOTECHNOLOGY VECTOR   (Russian Federation)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF ROCHESTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINOPURINE; ADENINE; CYTOSINE; DNA METHYLTRANSFERASE; DOUBLE STRANDED DNA; GUANINE; METHYL GROUP; S ADENOSYLHOMOCYSTEINE; S ADENOSYLMETHIONINE; SINEFUNGIN; THYMINE;

ALLOSTERISM; ARTICLE; BACTERIOPHAGE T4; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DNA HELIX; DNA METHYLATION; DNA STRAND; ENZYME BINDING; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE; FLUORESCENCE; NUCLEIC ACID BASE SUBSTITUTION; PRIORITY JOURNAL;

EID: 0035369787     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (40)

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2. Kinetic and catalytic mechanism of HhaI methyltransferase
3. Crystal structure of the HhaI DNA methyltransferase complexed with S-adenosyl-L-methionine
4. Crystal structure of the HhaI DNA methyltransferase
5. HhaI methyltransferase flips its target base out of the DNA helix
6. The crystal structure of HaeIII methyltransferase covalently complexed to DNA: An extrahelical cytosine and rearranged base pairing
7. Three-dimensional structure of the adenine-specific DNA methyltransferase M.TaqI in complex with the cofactor S-adenosylmethionine
8. Structure of PvuII DNA-(cytosine N4) methyltransferase, an example of domain permutation and protein fold assignment
9. Crystal structure of the DpnM DNA adenine methyltransferase from the DpnII restriction system of Streptococcus pneumoniae bound to S-adenosylmethionine
10. Structure of RsrI methyltransferase, a member of the N6-adenine β class of DNA methyltransferases
11. Structure of the N6-adenine DNA methyltransferase M.TaqI in complex with DNA and a cofactor analog
12. Pre-steady-state kinetics of bacteriophage T4 Dam DNA-[N6-adenine] methyltransferase: Interaction with native (GATC) or modified sites
13. Direct real time observation of base flipping by the EcoRI DNA methyltransferase
14. 2-Aminopurine as a fluorescent probe for DNA base flipping by methyltransferases
15. Targeted base stacking disruption by the EcoRI DNA methyltransferase
16. Functional roles of the conserved aromatic amino acid residues at position 108 (motif IV) and position 196 (motif VIII) in base flipping and catalysis by the N6-adenine DNA methyltransferase from Thermus aquaticus
17. Substrate binding in vitro and kinetics of RsrI [N6-adenine] DNA methyltransferase
18. Functional roles of the conserved threonine 250 in the target recognition domain of HhaI DNA methyltransferase
19. Chemical display of thymine residues flipped out by DNA methyltransferases
20. Mutational analysis of target base flipping by the EcoRV adenine-N6 DNA methyltransferase
21. + gene coding for phage T4 DNA adenine methylase
22. 6-adenine]-methyltransferase. Overexpression, purification and characterization
23. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
24. Calculation of protein extinction coefficients from amino acid sequence data
25. Dam methyltransferase from Escherichia coli: Kinetic studies using modified DNA oligomers: Nonmethylated substrates
26. Interaction of the phage T4 DNA-[N6-adenine]-(Dam)methyltransferase with oligonucleotides containing native or modified (defective) recognition sites
27. LAC9 DNA-binding domain coordinates two zinc atoms per monomer and contacts DNA as a dimer
28. The DNA binding domain of the rat liver nuclear protein C/EBP is bipartite
29. The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E.coli
30. Sequence-specific binding of DNA by the EcorV restriction and modification enzymes with nucleic acid and cofactor analogues
31. Sequence specific DNA binding by the MspI DNA methyltransferase
32. 6-adenine] methyltransferase
33. Fluorescence quenching study of equilibrium binding of phage T4 Dam DNA-(N6-adenine)-methyltransferase with substrates and ligands
34. A structural basis for the preferential binding of hemimethylated DNA by HhaI DNA methyltransferase
35. Reconciling structure and function in HhaI DNA cytosine-C-5 methyltransferase
36. Structures of HhaI methyltransferase complexed with substrates containing mismatches at the target base
37. HhaI and HpaII DNA methyltransferases bind DNA mismatches, methylate uracil and block DNA repair
38. Mechanism of inhibition of DNA (cytosine C5)-methyltransferase by oligodeoxyribonucleotides containing 5,6-dihydro-5-azacytosine
39. DNA containing 4′-thio-2′-deoxycytidine inhibits methylation by HhaI methyltransferase