Functional roles of the conserved aromatic amino acid residues at position 108 (Motif IV) and position 196 (Motif VIII) in base flipping and catalysis by the N6-adenine DNA methyltransferase from Thermus aquaticus

Biochemistry

Volumn 38, Issue 5, 1999, Pages 1426-1434

  Pues, Heike ^a,b   Bleimling, Nathalie ^a   Holz, Birgit ^a,c   Wölcke, Julian ^a,d   Weinhold, Elmar ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^b UNIVERSITY HOSPITAL ESSEN   (Germany)

^c PROCTER AND GAMBLE COMPANY   (United States)

^d EVOTEC BioSystems AG   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

2 AMINOPURINE; ADENINE; AROMATIC AMINO ACID; DNA METHYLTRANSFERASE; DOUBLE STRANDED DNA; METHYL GROUP; OLIGODEOXYNUCLEOTIDE; RNA METHYLTRANSFERASE; S ADENOSYLMETHIONINE;

ARTICLE; CATALYSIS; DNA SEQUENCE; FLUORESCENCE; MUTATION; NONHUMAN; PRIORITY JOURNAL; THERMUS AQUATICUS;

AMINO ACID SEQUENCE; BASE PAIR MISMATCH; CATALYSIS; CONSERVED SEQUENCE; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHENYLALANINE; POLYMERASE CHAIN REACTION; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); TAQ POLYMERASE; THERMUS; TRYPTOPHAN;

EID: 0033514427     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9818016     Document Type: Article

References (54)

1. Hotchkiss, R. D. (1948) J. Biol. Chem. 168, 315-332.
2. Wyatt, G. R. (1951) Biochem. J. 48, 581-584.
3. Dunn, D. B., and Smith, J. D. (1958) Biochem. J. 68, 627-636.
4. Janulaitis, A., Klimašauskas, S., Petrušyte, M., and Butkus, V. (1983) FEBS Lett. 161, 131-134.
5. Cheng, X. (1995) Annu. Rev. Biophys. Biomol. Struct. 24, 293-318.
6. Adams, R. L. P., and Burdon, R. H. (1985) Molecular Biology of DNA Methylation p 3, Springer-Verlag, New York.
7. Heitman, J. (1993) Genet. Eng. 15, 57-108.
8. Barras, F., and Marinus, M. G. (1989) Trends Genet. 5, 139-143.
9. Jost, J. P., and Saluz, H. P. (1993) DNA Methylation: Molecular Biology and Biological Significance, Brikhäuser Verlag, Basel, Switzerland.
10. Klimasauskas, S., Kumar, S., Roberts, R. J., and Cheng, X. (1994) Cell 76, 357-369.
11. Reinisch, K. M., Chen, L., Verdine, G. L., and Lipscomb, W. N. (1995) Cell 82, 143-153.
12. Wu, J. C., and Santi, D. V. (1987) J. Biol. Chem. 262, 4778-4786.
13. Chen, L., MacMillan, A. M., Chang, W., Ezaz-Nikpay, K., Lane, W. S., and Verdine, G. L. (1991) Biochemistry 30, 11018-11025.
14. Pogolotti, A. L., Ono, A., Subramaniam, R., and Santi, D. V. (1988) J. Biol. Chem. 263, 7461-7464.
15. Ho, D. K., Wu, J. C., Santi, D. V., and Floss, H. G. (1991) Arch. Biochem. Biophys. 284, 264-269.
16. Allan, B. W., and Reich, N. O. (1996) Biochemistry 35, 14757-14762.
17. Holz, B., Klimasaukas, S., Serva, S., and Weinhold, E. (1998) Nucleic Acids Res. 26, 1076-1083.
18. Labahn, J., Granzin, J., Schluckebier, G., Robinson, D. P., Jack, W. E., Schildkraut, I., and Saenger, W. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 10957-10961.
19. Gong, W., O'Gara, M., Blumenthal, R. M., and Cheng, X. (1997) Nucleic Acids Res. 25, 2702-2715.
20. Schluckebier, G., Labahn, J., Granzin, J., Schildkraut, I., and Saenger, W. (1995) Gene 157, 131-134.
21. Schluckebier, G., O'Gara, M., Saenger, W., and Cheng, X. (1995) J. Mol. Biol. 247, 16-20.
22. Malone, T., Blumenthal, R. M., and Cheng, X. (1995) J. Mol. Biol. 253, 618-632.
23. Pósfai, J., Bhagwat, A. S., Pósfai, G., and Roberts, R. J. (1989) Nucleic Acids Res. 17, 2421-2435.
24. Yu, L., Petros, A. M., Schnuchel, A., Zhong, P., Severin, J. M., Walter, K., Holzman, T. F., and Fesik, S. W. (1997) Nat. Struct. Biol. 4, 483-489.
25. Bussiere, D. E., Muchmore, S. W., Dealwis, C. G., Schluckebier, G., Nienaber, V. L., Edalji, R. P., Walter, K. A., Ladror, U. S., Holzman, T. F., and Abad-Zapatero, C. (1998) Biochemistry 37, 7103-7112.
26. McClelland, M. (1981) Nucleic Acids Res. 9, 6795-6804.
27. Schluckebier, G., Labahn, J., Granzin, J., and Saenger, W. (1998) Biol. Chem. 379, 389-400.
28. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
29. Pues, H., Holz, B., and Weinhold, E. (1997) Nucleic Acids Res. 25, 1303-1304.
30. Schluckebier, G., Kozak, M., Bleimling, N., Weinhold, E., and Saenger, W. (1997) J. Mol. Biol. 265, 56-67.
31. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254.
32. Reinstein, J., Vetter, I. R., Schlichting, I., Rösch, P., Wittinghofer, A., and Goody, R. S. (1990) Biochemistry 29, 7440-7450.
33. Leatherbarrow, R. J. (1992) GraFit Version 3.0, Erithacus Software Ltd., Staines, U.K.
34. Kunkel, T. A. (1985) Proc. Natl. Acad. Sci. U.S.A. 82, 488-492.
35. Kunkel, T. A., Roberts, J. D., and Zakour, R. A. (1987) Methods Enzymol. 154, 367-382.
36. Hemsley, A., Arnheim, N., Toney, M. D., Cortopassi, G., and Galas, D. J. (1989) Nucleic Acids Res. 17, 6545-6551.
37. Barnes, W. M. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 2216-2220.
38. Slatko, B. E., Benner, J. S., Jager-Quinton, T., Moran, L. S., Simcox, T. G., Van Cott, E. M., and Wilson, G. G. (1987) Nucleic Acids Res. 15, 9781-9796.
39. Kolb, A. I. (1988) Beckman Technical Bulletin, T-1650-NUC-88-6.
40. Ahmad, I., and Rao, D. N. (1996) J. Mol. Biol. 259, 229-240.
41. Saha, S., Ahmad, I., Reddy, Y. V. R., Krishnamurthy, V., and Rao, D. N. (1998) Biol. Chem. 379, 511-517.
42. Willcock, D. F., Dryden, D. T. F., and Murray, N. E. (1994) EMBO J. 13, 3902-3908.
43. Wölcke, J. (1998) Dissertation, University Dortmund, Germany.
44. Reich, N. O., and Mashhoon, N. (1991) Biochemistry 30, 2933-2939.
45. Gromova, E. S., Oretskaya, T. S., Eritja, R., and Guschlbauer, W. (1995) Biochem. Mol. Biol. Int. 36, 247-255.
46. Thielking, V., Du Bois, S., Eritja, R., and Guschlbauer, W. (1997) Biol. Chem. 378, 407-415.
47. Zinoviev, V. V., Evdokimov, A. A., Gorbunov, Y. A., Malygin, E. G., Kossykh, V. G., and Hattman, S. (1998) Biol. Chem. 379, 481-488.
48. Holz, B. (1998) Dissertation, University Dortmund, Germany.
49. Holz, B., Pues, H., Wölcke, J., and Weinhold, E. (1997) FASEB J. 11, A1151.
50. Serva, S., Klimašaukas, S., and Weinhold, E. (1997) Biologija 1997, 9-12.
51. Allan, B. W., Beechem, J. M., Lindstrom, W. M., and Reich, N. O. (1998) J. Biol. Chem. 273, 2368-2373.
52. Ward, D. C., Reich, E., and Stryer, L. (1969) J. Biol. Chem. 244, 1228-1237.
53. Fauchère, J.-L., and Pliška, V. (1983) Eur. J. Med. Chem. 18, 369-375.
54. Nicholls, A., Sharp, K. A., and Honig, B. (1991) Proteins 11, 281-296.