Excited state energy transfer pathways in photosynthetic reaction centers. 4. Asymmetric energy transfer in the heterodimer mutant

Journal of Physical Chemistry B

Volumn 105, Issue 9, 2001, Pages 1856-1862

  King, Brett A ^a   De Winter, Alex ^a   McAnaney, Tim B ^a   Boxer, Steven G ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHARGE TRANSFER; CHROMOPHORES; DIMERS; ELECTRON ENERGY LEVELS; ELECTRON TRANSITIONS; ENERGY TRANSFER; FLUORESCENCE; PHOTOSYNTHESIS; RATE CONSTANTS;

HETERODIMER MUTANTS;

BACTERIA;

EID: 0035280806     PISSN: 15206106     EISSN: None     Source Type: Journal    
DOI: 10.1021/jp002318j     Document Type: Article

References (40)

1. Breton, J.; Martin, J.-L.; Migus, A.; Antonetti, A.; Orszag, A. Proc. Natl. Acad. Sci. U.S.A. 1986, 83, 5121-5125.
2. Breton, J.; Martin, J.-L.; Fleming, G. R.; Lambry, J.-C. Biochemistry 1988, 27, 8276-8284.
3. Jia, Y.; Jonas, D. M.; Joo, T.; Nagasawa, Y.; Lang, M. J.; Fleming, G. R. J. Phys. Chem. 1995, 99, 6263-6266.
4. Jonas, D. M.; Lang, M. J.; Nagasawa, Y.; Joo, T.; Fleming, G. R. J. Phys. Chem. 1996, 700, 12660-12673.
5. Lin, S.; Taguchi, A. K. W.; Woodbury, N. W. J. Phys. Chem. 1996, 700, 17067-17078.
6. Stanley, R. J.; King, B. A.; Boxer, S. G. J. Phys. Chem. 1996, 700, 12052-12059.
7. King, B. A.; McAnaney, T. B.; de Winter, A.; Boxer, S. G. J. Phys. Chem. B 2000, 104, 8895-8902.
8. Kirmaier, C; Holten, D.; Bylina, E. J.; Youvan, D. C. Proc. Natl. Acad. Sci. U.S.A. 1988, 85, 7562-7566.
9. Hammes, S. L.; Mazzola, L.; Boxer, S. G.; Gaul, D. F.; Schenck, C. C. Proc. Natl. Acad. Sci. U.S.A. 1990, 87, 5682-5686.
10. Zhou, H. L.; Boxer, S. G. J. Phys. Chem. B 1997, 707, 57595766.
11. Laporte, L.; McDowell, L. M.; Kirmaier, C.; Schenck, C; Holten, D. Chem. Phys. 1993, 770, 615-629.
12. Kirmaier, C.; Bylina, E. J.; Youvan, D. C.; Holten, D. Chem. Phys. Lett. 1989, 759, 251-257.
13. McDowell, L. M.; Kirmaier, C.; Holten, D. J. Phys. Chem. 1991, 95, 3379-3383.
14. McDowell, L. M.; Gaul, D.; Kirmaier, C.; Holten, D.; Schenck, C. C. Biochemistry 1991, 30, 8315-8322.
15. McDowell, L. M.; Kirmaier, C.; Holten, D. Biochim. Biophys. Acta 1990, 239-246.
16. van Brederode, M. E.; van Stokkum, I. H. M.; Katilius, E.; van Mourik, F.; Jones, M. R.; van Grondelle, R. Biochemistry 1999, 33, 95569556.
17. Stanley, R. J.; Boxer, S. G. J. Phys. Chem. 1995, 99, 859-863.
18. The yield of 3D in M202HL is reported to be very low (Vrieze, J.; Schenck, C. C.; Hoff, A. J. Biochim. Biophys. Acta 1996, 7276, 229-238), and we confirmed that the absorption of D is not bleached on the 10 /
19. de Winter, A.; Boxer, S. G. J. Phys. Chem. B 1999, 103, 87868789.
20. Katilius, E.; Turanchik, T.; Lin, S.; Taguchi, A. K. W.; Woodbury, N. W. J. Phys. Chem. B 1999, 103, 7386-7389.
21. Goldsmith, J. O., Boxer, S. G. Biochim. Biophys. Acta 1996, 7276, 171-175.
22. Okamura, M. Y.; Isaacson, R. A.; Feher, G. Proc. Natl. Acad. Sci. U.S.A. 1975, 72, 3491-3495.
23. King, B. A.; Stanley, R. J.; Boxer, S. G. J. Phys. Chem. B 1997, 707, 3644-3648.
24. When the slow or fast component comprises a relatively small percentage of the total rise amplitude (<30%), the fit values for this component will be less accurate and will deviate from the values of 'B decay measured at 850 nm for direct excitation of B at 795 nm. In general, the slow component appears slower and the fast component appears faster and the values may lie outside the standard deviations reported for 'B decay. We note that such discrepancies are disguised in global analyses (common in the RC literature) where similar rate constants at different wavelengths are forced to converge upon a single value. Energy transfer to the highenergy feature or blue edge of D is likely following ultrafast relaxation (~100 fs) prior to emission (see Discussion and ref 24). For excitation in the B bands, where there is underlying absorption of D, direct excitation of D is unavoidable. Since we would not be able to resolve a 50-100 fs rise (relaxation of the initially excited state prior to emission) along with an ~190 fs rise ('B -D), the fit returns an intermediate value, depending on the relative populations of BM and D excited. This could explain why the rise of 'D in M202HL/M182HL for excitation at 802 nm appears faster than for excitation at 793 nm and why the rise of 'D is faster in general for excitation at the red edge of B compared with the blue edge.
25. Kirmaier, C.; Gaul, D.; Debey, R.; Holten, D.; Schenck, C. C. Science 1991, 257, 922-926.
26. Rubles, S. J.; Breton, J.; Youvan, D. C. Science 1990, 248, 14021405.
27. Eberl, U.; Gilbert, M.; Keupp, W.; Langenbacher, T.; Siegl, J.; Sinning, I.; Ogrodnik, A.; Robles, S. J.; Breton, J.; Youvan, D. C.; MichelBeyerle, M. E. Fast internal conversion in bacteriochlorophyll dimers; Breton, J., Vermeglio, A., Ed.; Plenum Press: New York, 1992; pp 25360.
28. Steffen, M. A.; Lao, K.; Boxer, S. G. Science 1994, 264, p 810816.
29. This result is reminiscent of what was observed in the M214LH ()6 and the M182HL (O)7 mutants, where changing the absorption band of the accessory chromophores (and presumably the spectral overlap of these bands with P) had little effect on the energy transfer rate as measured by the rise of 'P emission.
30. Chirino, A. J.; Lous, E. J.; Huber, M.; Allen, J. P.; Schenck, C. C; Paddock, M. L.; Feher, G.; Rees, D. C. Biochemistry 1994, 33, 4584-4593.
31. Jordanides, X. J.; Scholes, G. D.; Fleming, G. R. J. Pliys. Chem., in press.
32. Zhou, H. L.; Boxer, S. G. J. Phys. Chem. B 1998, 702, 91399147.
33. Zhou, H. L.; Boxer, S. G. J. Phys. Chem. B 1998, 702, 91489160.
34. van Brederode, M. E.; Jones, M. R.; van Grondelle, R. Chem. Phys. Lett. 1997, 268, 143-149.
35. Vos, M. H.; Breton, J.; Martin, J.-L. J. Phys. Chem. B 1997, 101, , 9820-9832.
36. Lin, S.; Jackson, J.; Taguchi, A. K. W.; Woodbury, N. W. J. Phys. Chem. B 1998, 702, 4016-4022.
37. Lao, K.; Moore, L. J.; Zhou, H.; Boxer, S. G. J. Phys. Chem. 1995, 99, 496.
38. van Brederode, M. E.; Jones, M. R.; van Mourik, F.; van Stokkum, I. H. M.; van Grondelle, R. Biochemistry 1997, 36, 6855-6861.
39. Differences in the kinetics between the QA-reduced and QA-depleted heterodimer RCs are seen in the ratios of the amplitudes of the slow and fast components of 'B emission. Essentially identical ratios between QAreduced and QA-depleted RCs when measuring the rise of 'D at 1040 nm indicates that there are not significant differences in the relative absorption of L and M side chromophores at the excitation wavelengths. One explanation of the amplitude differences in 'B emission is that subtle changes occur in the BL and/or BM emission bands between QA-reduced and QAdepleted RCs.
40. Deisenhofer, J.; Epp, O.; Sinning, L; Michel, H. J. Mol. Biol. 1995, . 246, 429-457.