Effects of recombinant ovine leptin on in vitro lipolysis and lipogenesis in subcutaneous adipose tissue from lactating and nonlactating sheep

Journal of Animal Science

Volumn 79, Issue 2, 2001, Pages 445-452

  Newby, D ^a   Gertler, A ^b   Vernon, R G ^a  

^a HANNAH RESEARCH INSTITUTE   (United Kingdom)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Adipose Tissue; Leptin; Lipogenesis; Lipolysis; Sheep

Indexed keywords

ADENOSINE DEAMINASE; BETA ADRENERGIC RECEPTOR STIMULATING AGENT; DNA BINDING PROTEIN; GROWTH HORMONE; ISOPRENALINE; LEPTIN; LIPID; MILK PROTEIN; PHENYLISOPROPYLADENOSINE; RECOMBINANT PROTEIN; STAT3 PROTEIN; STAT5 PROTEIN; TRANSACTIVATOR PROTEIN;

ADIPOCYTE; ADIPOSE TISSUE; ANIMAL; ANIMAL DISEASE; ARTICLE; BIOSYNTHESIS; CULTURE TECHNIQUE; DRUG EFFECT; FEMALE; LACTATION; LIPOLYSIS; MALE; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY; SHEEP; SIGNAL TRANSDUCTION; WESTERN BLOTTING;

ADENOSINE DEAMINASE; ADIPOCYTES; ADIPOSE TISSUE; ADRENERGIC BETA-AGONISTS; ANIMALS; BLOTTING, WESTERN; CULTURE TECHNIQUES; DNA-BINDING PROTEINS; FEMALE; GROWTH HORMONE; ISOPROTERENOL; LACTATION; LEPTIN; LIPIDS; LIPOLYSIS; MALE; MILK PROTEINS; PHENYLISOPROPYLADENOSINE; PHOSPHORYLATION; RECOMBINANT PROTEINS; SHEEP; SIGNAL TRANSDUCTION; STAT3 TRANSCRIPTION FACTOR; STAT5 TRANSCRIPTION FACTOR; TRANS-ACTIVATORS;

EID: 0035256613     PISSN: 00218812     EISSN: None     Source Type: Journal    
DOI: 10.2527/2001.792445x     Document Type: Article

References (33)

1. Bai, Y., S. Zhang, K-S. Kim, J.-K. Lee, and K.-H. Kim. 1996. Obese gene expression alters the ability of 30A5 preadipocytes to respond to lipogenic hormones. J. Biol. Chem. 271:13939-13942.
2. Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72:248-254.
3. Campbell, G. S., D. J. Meyer, R. Raz, D. E. Levy, J. Schwartz, and C. Carter-Su. 1995. Activation of acute phase response factor (APRF)/Stat 3 transcription factor by growth hormone. J. Biol. Chem. 270:3974-3979.
4. Campfield, L. A., F. J. Smith, Y. Guisez, R. Devos, and P. Burn. 1995. Recombinant mouse OB protein: Evidence for a peripheral signal linking adiposity and central neural networks. Science (Wash DC) 269:546-549.
5. Delavaud, C., F. Bocquier, Y. Chilliard, D. H. Keisler, A. Gertler, and G. Kann. 2000. Plasma leptin determination in ruminants: effect of nutritional status and body fatness on plasma leptin concentration assessed by a specific RIA in sheep. J. Endocrinol. 165:519-526.
6. Dyer, C. J., J. M. Simmons, R. L. Matteri, and D. H. Keisler. 1997. Leptin receptor mRNA is expressed in ewe anterior pituitary and adipose tissues and is differentially expressed in hypothalamic regions of well-fed and feed-restricted ewes. Domest. Anim. Endocrinol. 14:119-128.
7. Folch, J., M. Lees, and G. H. S. Stanley. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226:497-509.
8. Frederich, R. C., B. Lollmann, A. Hamann, A. Napolitano-Rosen, B. B. Kahn, B. B. Lowel, and J. S. Flier. 1995. Expression of ob mRNA and its encoded protein in rodents. Impact of nutrition and obesity. J. Clin. Investig. 96:1658-1663.
9. Fruhbeck, G., M. Aguado, J. Gomez-Ambrosi, and J. A. Martinez. 1998. Lipolytic effect of in vivo leptin administration on adipocytes of lean and ob/ob mice, but not db/db mice. Biochem. Biophys. Res. Commun. 250:99-102.
10. Fruhbeck, G., M. Aguado, and J. A. Martinez. 1997. In vitro lipolytic effect of leptin on mouse adipocytes: Evidence for a possible autocrine/paracrine role of leptin. Biochem. Biophys. Res. Commun. 240:590-594.
11. Gertler, A., J. Simmons, and D. H. Keisler. 1998. Large-scale preparation of biologically active recombinant ovine obese protein (leptin). FEBS Letters 422:137-140.
12. Halaas, J. L., K. S. Gakiwala, M. Maffei, S. L. Cohen, B. T. Chait, D. Rabinowtz, R. L. Lallone, S. K. Burley, and J. M. Friedman. 1995. Weight-reducing effects of the plasma protein encoded by the obese gene. Science (Wash. DC) 269:543-546.
13. Hanson, R. W., and F. J. Ballard. 1968. Citrate, pyruvate and lactate contaminants of commercial serum albumin. J. Lipid Res. 9:667-668.
14. Houseknecht, K. L., and C. P. Portocarrero. 1998. Leptin and its receptors: Regulators of whole-body energy homeostasis. Domest. Anim. Endocrinol. 15:457-475.
15. Krebs, H. A., and K. Henseleit. 1933. Urea formation in the animal body. Hoppe-Seyler's Z. Physiol. Chem. 210:33-66.
16. Mick, G., T. Vanderbloomer, C. L. Fu, and K. McCormick. 1998. Leptin does not affect adipocyte glucose metabolism: Studies in fresh and cultured adipocytes. Metabolism 47:1360-1365.
17. Morrison, C. D., J. A. Daniel, B. J. Holmberg, O. U. Bolden, N. Raver, A. Gertler, and D. H. Keisler. 1998. Effects of lateral cerebroventricular infusion of leptin on ewe lambs. J. Anim. Sci. 76(Suppl. 1):225.
18. Mostyn, A., E. J. Dastoor, T. Stephenson, R. Webb, D. H. Keisler, and M. E. Symonds. 2000. Effect of acute leptin administration on thermoregulation in day old lambs during non-rapid eye movement sleep. Proc. Nutr. Soc. 59:13a.
19. Muller, G., J. Ertl, M. Gerl, and G. Preibisch. 1997. Leptin impairs metabolic actions of insulin in isolated rat adipocytes. J. Biol. Chem. 272:10585-10593.
20. Pelleymounter, M. A., M. J. Cullen, M. B. Baker, R. Hecht, D. Winters, T. Boone, and F. Collins. 1995. Effects of the obese gene product on body weight regulation in ob/ob mice. Science (Wash DC) 269:540-543.
21. Plested, C. P., E. Taylor, D. N. Brindley, and R. G. Vernon. 1987. Interactions of insulin and dexamethasone in the control of pyruvate activity and glucose metabolism in sheep adipose tissue. Biochem. J. 247:459-465.
22. Ranganathan, S., T. P. Ciaraldi, R. R. Henry, S. Mudaliar, and P. A. Kern. 1998. Lack of effect of leptin on glucose transport, lipoprotein lipase, and insulin action in adipose and muscle cells. Endocrinology 139:2509-2513.
23. Robertson, J. P., A. Faulkner, and R. G. Vernon. 1982. Regulation of glycolysis and fatty acid synthesis from glucose in sheep adipose tissue. Biochem. J. 206:577-586.
24. Schwartz, M. W., R. J. Seeley, L. A. Campfield, P. Burn, and D. G. Baskin. 1996. Identification of targets of leptin action in rat hypothalamus. J. Clin. Investig. 98:1101-1106.
25. Siegrist-Kaiser, C. A., V. Pauli, C. E. Juge-Aubry, O. Boss, A. Pernin, W. W. Chin, I. Cusin, F. Rohner-Jeanrenaud, A. G. Burger, J. Zapf, and C. A. Meier. 1997. Direct effects of leptin on brown and white adipose tissue. J. Clin. Investig. 100:2858-2864.
26. Soret, B., H.-J. Lee, E. Finley, S. C. Lee, and R. G. Vernon. 1999. Regulation of differentiation of sheep subcutaneous and abdominal preadipocytes in culture. J. Endocrinol. 161:517-524.
27. Stephens, T. W., M. Basinki, P. K. Bristow, J. M. Bue-Valleskey, S. G. Burgett, L. Craft, J. Hale, J. Hoffmann, H. M. Hsiung, A. Kriauciunas, W. MacKellar, P. R. Rosteck, Jr., B. Schoner, D. Smith, F. C. Tinsley, X. Y. Zhang, and M. Heiman. 1995. The role of neuropeptide Y in the antiobesity action of the obese gene product. Nature (Lond.) 377:530-532.
28. Vernon, R. G., R. A. Clegg, and D. J. Flint. 1981. Metabolism of sheep adipose tissue during pregnancy and lactation: Adaptation and regulation. Biochem. J. 200:307-314.
29. Vernon, R. G., and E. Finley. 1988. Roles of insulin and growth hormone in the adaptations of fatty acid synthesis in white adipose tissue during the lactation cycle in sheep. Biochem. J. 256:873-878.
30. Wang, M.-Y., Y. Lee, and R. H. Unger. 1999. Novel form of lipolysis induced by leptin. J. Biol. Chem. 274:17541-17544.
31. Wood, T. J. J., L.-A. Hakdosen, D. Sliva, M. Sundstrom, and G. Norstedt. 1997. Stimulation of kinase cascades by growth hormone: A paradigm for cytokine signalling. Prog. Nucleic Acid Res. Mol. Biol. 57:73-94.
32. Zhang, Y., R. Proenca, M. Maffei, M. Barone, L. Leopold, J. and M. Friedman. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature (Lond) 372:425-432.
33. Zierath, J. R., E. U. Frevert, J. W. Ryder, P.-O. Berggren, and B. B. Kahn. 1998. Evidence against a direct effect of leptin on glucose transport in skeletal muscle and adipocytes. Diabetes 47:1-4.