Topography of lacUV5 initiation complexes

Nucleic Acids Research

Volumn 29, Issue 3, 2001, Pages 854-861

  Studitsky, Vasily M ^a   Brodolin, Konstantin L ^b   Liu, Ye ^a   Mirzabekov, Andrei D ^c  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b INSTITUTE OF MOLECULAR GENETICS   (Russian Federation)

^c ENGELHARDT INSTITUTE OF MOLECULAR BIOLOGY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; DNA; RNA POLYMERASE;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; DNA CROSS LINKING; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE REARRANGEMENT; GENETIC ANALYSIS; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN CROSS LINKING; PROTEIN DNA BINDING; PROTEIN INTERACTION; TOPOGRAPHY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035253626     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/29.3.854     Document Type: Article

References (45)

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2. Changes in the DNA structure of the lac UV5 promoter during formation of an open complex with Escherichia coli RNA polymerase
3. Temperature dependence of the rate constants of the Escherichia coli RNA polymerase-lambda PR promoter interaction. Assignment of the kinetic steps corresponding to protein conformational change and DNA opening
4. DNA footprints of the two kinetically significant intermediates in formation of an RNA polymerase-promoter open complex: Evidence that interactions with start site and downstream DNA induce sequential polymerase and DNA
5. The kinetics of sigma subunit directed promoter recognition by E. coli RNA polymerase
6. Conformational changes in E. coli RNA polymerase during promoter recognition
7. Quantitative analysis of multiple-hit footprinting studies to characterize DNA conformational changes in protein-DNA complexes: Application to DNA opening by E sigma-70 RNA polymerase
8. Mapping of single-stranded regions in duplex DNA at the sequence level: Single-strand-specific cytosine methylation in RNA polymerase-promoter complexes
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10. Activation and repression of transcription by differential contact: Two sides of a coin
11. Protein-nucleic acid interactions during open complex formation investigated by systematic alteration of the protein and DNA binding partners
12. Structural organization of the RNA polymerase-promoter open complex
13. Mapping the sigma 70 subunit contact sites on Escherichia coli RNA polymerase with a sigma 70-conjugated chemical protease
14. Identification of RNA polymerase beta' subunit segment contacting the melted region of the lacUV5 promoter
15. A structural model of transcription elongation
16. Mapping DNA-protein interactions by cross-linking
17. Coli RNA polymerase interacts homologously with two different promoters
18. Contacts between Escherichia coli RNA polymerase and a lac operon promoter
19. A procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography
20. Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase
21. Topography of interaction of Escherichia coli RNA polymerase subunits with lac UV5 promoter
22. Sequencing end-labeled DNA with base-specific chemical cleavages
23. Characterization of the closed complex intermediate formed during transcription initiation by Escherichia coli RNA polymerase
24. Region I of sigma 70 is required for efficient isomerization and initiation of transcription by Escherichia coli RNA polymerase
25. Stimulation of open complex formation by nicks and apurinic sites suggests a role for nucleation of DNA melting in Escherichia coli promoter function
26. Structural studies of lacUV5-RNA polymerase interactions in vitro. Ethylation interference and missing nucleoside analysis
27. The 0 degree C closed complexes between Escherichia coli RNA polymerase and two promoters, T7-A3 and lacUV5
28. Direct evidence for the preferential binding of Escherichia coli RNA polymerase holoenzyme to the ends of deoxyribonucleic acid restriction fragments
29. A single polypeptide possesses the binding and transcription activities of the adenovirus major late transcription factor
30. Flexibility of the DNA enhances promoter affinity of Escherichia coli RNA polymerase
31. High-resolution analysis of lac transcription complexes inside cells
32. Protein-DNA cross-linking at the lac promoter
33. Altered promoter recognition by mutant forms of the sigma 70 subunit of Escherichia coli RNA polymerase
34. A mutant Escherichia coli sigma 70 subunit of RNA polymerase with altered promoter specificity
35. Mapping the promoter DNA sites proximal to conserved regions of sigma 70 in an Escherichia coli RNA polymerase-lacUV5 open promoter complex
36. Uranyl mediated photofootprinting reveals strong E.coli RNA polymerase-DNA backbone contacts in the +10 region of the DeoP1 promoter open complex
37. Topography of intermediates in transcription initiation of E.coli
38. Development of RNA polymerase-promoter contacts during open complex formation
39. Intermediates in the formation of the open complex by RNA polymerase holoenzyme containing the sigma factor sigma 32 at the groE promoter
40. Base flipping
41. Escherichia coli RNA polymerase, deleted in the C-terminal part of its alpha-subunit, interacts differently with the cAMP-CRP complex at the lacP1 and at the galP1 promoter
42. A third recognition element in bacterial promoters: DNA binding by the alpha subunit of RNA polymerase
43. Open complex formation during transcription initiation at the Escherichia coli gal P1 promoter: The role of the RNA polymerase alpha subunit at promoters lacking an UP-element
44. Three-dimensional structure of E. coli core RNA polymerase: Promoter binding and elongation conformations of the enzyme
45. Crystal structure of Thermus aquaticus core RNA polymerase at 3.3 Å resolution