Quantitation of alternatively spliced estrogen receptor alpha mRNAs as separate gene populations

Steroids

Volumn 66, Issue 1, 2001, Pages 17-23

  Koduri, Sailaja ^a   Poola, Indra ^a  

^a HOWARD UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Estrogen receptor alpha; mRNA quantitation; Splice targeted primers; Splice variants; Template competition RT PCR

Indexed keywords

COMPLEMENTARY DNA; ESTROGEN RECEPTOR; ESTROGEN RECEPTOR ALPHA; MESSENGER RNA;

ARTICLE; BREAST CANCER; CANCER CELL; CELL STRAIN MCF 7; CONTROLLED STUDY; EXON; GENE; HUMAN; HUMAN CELL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA SPLICING;

ALTERNATIVE SPLICING; BREAST NEOPLASMS; DNA PRIMERS; ESTROGEN RECEPTOR ALPHA; EXONS; FEMALE; GENE DOSAGE; HUMANS; RECEPTORS, ESTROGEN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR CELLS, CULTURED;

MINK CELL FOCUS-FORMING VIRUS;

EID: 0035238763     PISSN: 0039128X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0039-128X(00)00128-8     Document Type: Article

References (19)

1. Estrogen receptor variants in clinical breast cancer
2. Coexpression of multiple estrogen receptor variant messenger RNAs in normal and neoplastic breast tissues and MCF-7 cells
3. Prevalence of estrogen receptor variant messenger RNAs in breast cancer
4. Oestrogen receptor variants and mutations in human breast cancer
5. Expression of a constitutively active estrogen receptor variant in the estrogen receptor negative BT-20 human breast cancer cell line
6. Models of estrogen receptor regulation by estrogens and anti-estrogens in breast cancer cell lines
7. Expression of wild type estrogen receptor beta and variant isoforms in human breast cancer
8. Variant human breast tumor estrogen receptor with constitutive transcriptional activity
9. Inhibition of estrogen receptor action by a naturally occurring variant in human breast tumors
10. Differential expression of wild type and variant ER mRNAs by stocks of MCF-7 breast cancer cells may account for differences in estrogen responsiveness
11. Loss of an estrogen receptor isoform (ER alpha delta 3) in breast cancer and the consequence of its re-expression; interference with estrogen-stimulated properties of malignant transformation
12. Primer design strategies for the amplification of alternatively spliced molecules
13. Alterations in the estrogen receptor alpha mRNA in the breast tumors of African American women
14. Analysis of cytokine mRNA and DNA; detection and quantitation by competitive PCR
15. Human estrogen receptor cDNA; sequence, expression, and homology to v-erb-A
16. Quantitation of estrogen receptor mRNA copy numbers in breast cancer cell lines and tumors
17. Comparison of the ligand specificity and transcript tissue distribution of estrogen receptor alpha and beta
18. Differential ligand activation of estrogen receptors ERα and ERβ in AP-1 sites
19. MCF-7/LCC2; a 4-hydroxytamoxifen resistant human breast cancer variant that remains sensitive to the steroidal antiestrogen ICI