Rapid PCR-mediated synthesis of competitor molecules for accurate quantification of β2 GABAA receptor subunit mRNA

Brain Research Protocols

Volumn 8, Issue 3, 2001, Pages 184-190

  Vela, José ^a   Vitorica, Javier ^a   Ruano, Diego ^a  

^a UNIVERSITY OF SEVILLE   (Spain)

Author keywords

Development; GABAA receptor; Internal standard; RNA quantification; RT competitive PCR

Indexed keywords

4 AMINOBUTYRIC ACID A RECEPTOR; MESSENGER RNA; PRIMER RNA; RECEPTOR SUBUNIT;

ACCURACY; ANIMAL TISSUE; ARTICLE; BRAIN DEVELOPMENT; CONTROLLED STUDY; HIPPOCAMPUS; MALE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; RNA SEQUENCE; RNA SYNTHESIS; SITE DIRECTED MUTAGENESIS; STANDARD;

ANIMALS; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; GABA ANTAGONISTS; HIPPOCAMPUS; INDICATORS AND REAGENTS; MALE; RATS; RATS, WISTAR; RECEPTORS, GABA-A; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 0035216995     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(01)00109-X     Document Type: Article

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4. Quantitative analysis of mu and delta opioid receptor gene expression in rat brain and peripheral ganglia using competitive polymerase chain reaction
5. Absolute quantitation of γ-aminobutyric acid A receptor subunit messenger RNAs by competitive polymerase chain reaction
6. Rapid quantification of gene expression by competitive RT-PCR and ion-pair reversed-phase HPLC
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8. Regulation of gamma-aminobutyric acid A receptor subunit expression by activation of N-methyl-D-aspartate-selective glutamate receptors
9. A receptor subunit mRNAs by layer V pyramidal cells of the rat primary visual cortex
10. A and alpha-amino-3-hydroxy-5-methylsoxazole-4-propionate receptors are differentially affected by aging in the rat hippocampus
11. Kainate receptor subunits expressed in single cultured hippocampal neurons: Molecular and functional variants by RNA editing
12. PCR-mediated synthesis of exogenous competitors for quantitative RT-PCR
13. A receptor beta subunit heterogeneity: Functional expression of cloned cDNAs