Rapid quantification of gene expression by competitive RT-PCR and ion-pair reversed-phase HPLC

BioTechniques

Volumn 20, Issue 2, 1996, Pages 250-257

  Hayward Lester, Amanda ^a,c   Oefner, Peter J ^b   Doris, Peter A ^a  

^a TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER   (United States)

^b STANFORD UNIVERSITY   (United States)

^c TEXAS TECH UNIVERSITY HEALTH SCIENCES CENTER SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ANGIOTENSINOGEN; COMPLEMENTARY DNA; ISOENZYME; MUTANT PROTEIN; RNA;

AGAR GEL ELECTROPHORESIS; ANIMAL TISSUE; ARTICLE; BLOOD; CONTROLLED STUDY; GENE EXPRESSION; ION PAIR CHROMATOGRAPHY; KIDNEY; NONHUMAN; PROTEIN EXPRESSION; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ANGIOTENSINOGEN; ANIMALS; BASE SEQUENCE; BRAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA PRIMERS; ELECTROPHORESIS, AGAR GEL; GENE EXPRESSION; ISOENZYMES; KIDNEY; LINEAR MODELS; MOLECULAR SEQUENCE DATA; NA(+)-K(+)-EXCHANGING ATPASE; POLYMERASE CHAIN REACTION; RATS; RNA;

ANIMALIA;

EID: 0030020279     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96202rr02     Document Type: Article

References (24)

1. Becker-André, M. and K.Hahlbrock. 1989. Absolute mRNA quantification using the polymerase chain reaction (PCR). Nucleic Acids. Res. 17:9437-9446.
2. Bonn, G., C. Huber and P. Oefner, inventors. 1994. Verfahren zur trennung von nucleinsaeuren (Methods for the separation of nucleic acids) Austrian patent 398 973.
3. Chang, A., J. Zhao and M. Krajden. 1994. Polymerase chain reaction kinetics when using a positive internal control target to quantitatively detect cytomegalovirus target sequences. J. Virol. Methods 48:223-236
4. deKant, E., C.F. Rochlitz and R. Herrmann. 1994. Gene expression analysis by a competitive and differential PCR with antisense competitors. BioTechniques 17:934-942
5. Dostal, D.E., K.N. Rothblum and K.M. Baker. 1994. An improved method for absolute quantification of mRNA using multiplex polymerase chain reaction. determination of renin and angiotensinogen mRNA levels in various tissues Anal. Biochem. 223:239-250.
6. Ferré, F., A. Marchese, P. Pezzoli, S. Griffin, E. Buxton and V. Boyer. 1994. Quantitative PCR: an overview. In K.B. Mullis, F. Ferré and R.A. Gibbs (Eds ), The Polymerase Chain Reaction. Birkhauser, Boston.
7. Ferré, F., A.L. Marchese, S.L. Griffin, A.E. Daigle, S.P. Richieri, F.C. Jensen and D.J. Carlo. 1993. Development and validation of a polymerase chain reaction method for the precise quantitation of HIV-1 DNA in blood cells from subjects undergoing a 1-year immunotherapeutic treatment. AIDS 7 (2 Suppl): S21-S27.
8. Gilliland, G., S. Perrin, K. Blanchard and H.F. Bunn. 1990. Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87:2725-2729.
9. Huber, C.G., P.J. Oefner and G.K. Bonn. 1993. Rapid analysis of biopolymers on modified nonporous polystyrene-divinylbenzene particles. Chromatographia 37:653-658.
10. Huber, C.G., P.J. Oefner and G.K. Bonn. 1995. Rapid and accurate sizing of DNA fragments on alkylated nonporous poly(styrene-divinylbenzene) particles. Anal. Chem. 67:578-585.
11. Huber, C.G., P.J. Oefner, E. Preuss and G.K. Bonn. 1993. High-resolution liquid chromatography of DNA fragments on non-porous poly(styrene-divinylbenzene) particles [published erratum appears in Nucleic Acids Res., May 11, 1993; 21:2284]. Nucleic Acids Res 21:1061-1066.
12. Ikonen, E., T. Manninen, L. Peltonen and A.-C. Syvanen. 1994. Quantitative determination of rare mRNA species by PCR and solid-phase minisequencing. PCR Methods Appl. 1:234-240.
13. Katz, E.D. and L.A. Haff. 1990. Rapid separation, quantitation and purification of products of polymerase chain reaction by liquid chromatography. J. Chromatogr 512:433-444.
14. Landgraf, A., B. Reckmann and A. Pingoud. 1991. Quantitative analysis of polymerase chain reaction (PCR) products using primers labeled with biotin and a fluorescent dye. Anal. Biochem. 193:231-235.
15. Noonan, K.E., C. Beck, T.A. Holzmayer, J.E. Chin, J.S. Wunder, I.L. Andrulis, A.F. Gazdar, C.L. Willman, B. Griffith, D.D. Von Hoff and I.B. Roninson. 1990. Quantitative analysis of MDR1 gene expression in human tumors by polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87:7160-7164.
16. Oefner, P.J., C.G. Huber, E. Puchhammer-Stöckl, F. Umlauft, K. Grünewald, G.K. Bonn and C. Kunz. 1994. High-performance liquid chromatography for routine analysis of hepatitis C virus cDNA/PCR products. BioTechniques 16:898-908.
17. Piatak Jr., M., K.-C. Luk, B. Williams and J.D. Lifson. 1993. Quantitative competitive polymerase chain reaction for accurate quantification of HIV DNA and RNA species. BioTechniques 14:70-80.
18. Raeymaekers, L. 1993. Quantitative PCR: theoretical considerations with practical implications. Anal. Biochem. 214:582-585.
19. Schmitt, J.F., M. Guthridge, C. Economou, J. Bertolini and M.T.W. Hearn. 1992. A new quantitative polymerase chain reaction-high performance ion exchange liquid Chromatographic method for the detection of fibroblast growth factor-beta gene amplification J. Biochem. Biophys. Methods 24:119-133
20. Schneeberger, C., P. Speiser, F. Kury and R. Zeillinger. 1995. Quantitative detection of reverse transcriptase-PCR products by means of a novel and sensitive DNA stain. PCR Methods Appl. 4:234-238.
21. Siebert, P.D. and J.W. Larrick. 1992. Competitive PCR Nature 359:557-558.
22. Simmonds, P., P. Balfe, J.F. Peutherer, C.A. Ludlam, J.O. Bishop and A.J. Leigh Brown. 1990. Human immunodeficiency virus-infected individuals contain provirus in small numbers of peripheral mononuclear cells and at low copy numbers. J. Virol. 64:864-872.
23. Volkenandt, M., A.P. Dicker, D. Banerjee, R. Fanin, B. Schweitzer, T. Horikoshi, K. Danenberg, P. Danenberg and J.R. Bertino. 1992. Quantitation of gene copy number and mRNA using the polymerase chain reaction. Proc Soc. Exp. Biol. Med. 200:1-6.
24. Wei, J., E.A. Walton, A. Milici and J.J. Buccafusco. 1994. m1-m5 Muscarinic receptor distribution in rat CNS by RT-PCR and HPLC. J. Neurochem 63:815-821.