Quantitative single-cell-reverse transcription-PCR demonstrates that A- current magnitude varies as a linear function of shal gene expression in identified stomatogastric neurons

Journal of Neuroscience

Volumn 17, Issue 17, 1997, Pages 6597-6610

  Baro, Deborah J ^a,b   Levini, Robert M ^a   Kim, Marshall T ^a   Willms, Allan R ^b   Lanning, Cathy Cole ^a   Rodriguez, Hilda E ^a   Harris Warrick, Ronald M ^a  

^a Seeley G Mudd Hall   (United States)

^b Cornell University ^*  (United States)

Author keywords

Gene regulation; Identified neuron; Invertebrate; Kv; Noncompetitive PCR; Potassium channel; Pyloric network; Quantitative; Shaker family; Shal; Single cell RT PCR; Stomatogastric; Transcriptional control; Transient potassium current

Indexed keywords

POTASSIUM CHANNEL;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; GENE CONTROL; GENE EXPRESSION REGULATION; LOBSTER; NONHUMAN; POTASSIUM CURRENT; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION REGULATION;

EID: 0030765507     PISSN: 02706474     EISSN: None     Source Type: Journal    
DOI: 10.1523/jneurosci.17-17-06597.1997     Document Type: Article

References (40)

1. + current in a subset of neurons. Neuron 2:129-140.
2. A) in piriform cortex. J Neurosci 16:3862-3876.
3. Baro DJ, Coniglio LM, Cole CL, Rodriguez HE, Lubell JK, Kim MT, Harris-Warrick RM (1996a) Lobster shal: comparison with Drosophila shal and native potassium currents in identified neurons. J Neurosci 16:1689-1701.
4. Baro DJ, Cole CL, Harris-Warrick RM (1996b) RT-PCR analysis of shaker, shab, shaw, and shal gene expression in single neurons and glial cells. Receptors Channels 4:149-159.
5. Barrantes FJ (1993) Structural-functional correlates of the nicotinic acetylcholine receptor and its lipid microenvironment. FASEB J 7:1460-1467.
6. Bhushan A, McNamee MG (1993) Correlation of phospholipid structure with functional effects on the nicotinic acetylcholine receptor. Biophys J 64:716-723.
7. + channel mRNA in isolated ferret cardiac myocytes. Circ Res 78:1083-1089.
8. A) on synaptic potentials in sympathetic ganglion cells of the guinea-pig. J Physiol (Lond) 374:273-288.
9. Cassell JF, Clark AL, McLachlan EP (1986) Characteristics of phasic and tonic sympathetic ganglion cells of the guinea-pig. J Physiol (Lond) 372:457-483.
10. + channel. J Gen Physiol 102:713-728.
11. Chandy CK, Gutman GA (1995) Voltage-gated potassium channel genes. In: Handbook of receptors and channels: ligand- and voltagegated ion channels (North RA, ed), pp 1-71. Boca Raton: CRC.
12. Chang HM, Reitstetter R, Gruener R (1995) Lipid-ion channel interactions: increasing phospholipid headgroup size, but not ordering acyl chains, alters reconstituted channel behavior. J Membr Biol 145:13-19.
13. + channel subunits from Shaker and eag families in Xenopus oocytes. Neuron 17:535-542.
14. Chouinard SW, Wilson GF, Schlimgen AK, Ganetzky B (1995) A potassium channel β-subunit related to the aldo-keto reductase superfamily is encoded by the Drosophila hyperkinetic locus. Proc Natl Acad Sci USA 92:6763-6767.
15. Chung S, Saal DB, Kaczmarek LK (1995) Elimination of potassium channel expression by antisense oligonucleotides in a pituitary cell line. Proc Natl Acad Sci USA 92:5955-5959.
16. + channel Kir 2.3 to PSD-95 is regulated by protein kinase A phosphorylation. Neuron 17:759-767.
17. Connor JA (1975) Neural repetitive firing: a comparative study of membrane properties of crustacean walking leg axons. J Neurophysiol 351:922-932.
18. Coronado R, Latorre R, Mautner HG (1984) Single potassium channels with delayed rectifier behavior from lobster axon membranes. Biophys J 45:289-299.
19. + current systems. Neuron 7:763-773.
20. Curtis D, Lehmann R, Zamore PD (1995) Translational regulation in development. Cell 81:171-178.
21. Deal KK, Lovinger DM, Tamkun MM (1994) The brain Kvl.1 potassium channel: in vitro and in vivo studies on subunit assembly and post-translational processing. J Neurosci 14:1666-1676.
22. Decker CJ, Parker R (1995) Diversity of cytoplasmic functions for the 3′ untranslated region of eukaryotic transcripts. Curr Opin Cell Biol 7:386-392.
23. + channel messenger RNAs from different subfamilies. J Neurosci 12:538-548.
24. Dixon JE, McKinnon D (1994) Quantitative analysis of potassium channel mRNA expression in atrial and ventricular muscles of rats. Circ Res 75:252-260.
25. Dixon JE, McKinnon D (1996) Potassium channel mRNA expression in prevertebral and paravertebral sympathetic neurons. Eur J Neurosci 8:183-191.
26. Eisen JS, Marder E (1982) Mechanisms underlying pattern generation in lobster stomatogastric ganglion as determined by selective inactivation of identified neurons. III. Synaptic connections of electrically coupled pyloric neurons. J Neurophysiol 48:1392-1415.
27. + channel β subunit expressed in human heart. Proc Natl Acad Sci USA 92:6309-6313.
28. Ferre F (1992) Quantitative or semi-quantitative PCR: reality versus myth. PCR Methods Appl 2:1-9.
29. Foley KR, Leonard MW, Engel JD (1993) Quantitation of RNA using the polymerase chain reaction. Trends Genet 9:380-385.
30. Furakawa Y, Kandel E, Pfaffinger P (1992) Three types of early transient potassium currents in Aplysia neurons. J Neurosci 12:989-1000.
31. Gause WC, Adamovicz J (1994) The use of PCR to quantitate gene expression. PCR Methods Appl 3[Suppl 6]:123-135.
32. Graubard K, Hartline DK (1991) Voltage-clamp analysis of intact stomatogastric neurons. Brain Res 557:241-254.
33. Hamill OP, Huguenard JJ, Prince DA (1991) Patch-clamp studies of voltage-gated currents in identified neurons of the rat cerebral cortex. Cereb Cortex 1:48-61.
34. Harris-Warrick RM, Marder E, Selverston AI, Moulins M (1992) Dynamic biological networks: the stomatogastric nervous system. Cambridge, MA: MIT.
35. Harris-Warrick RM, Coniglio LM, Barazangi N, Guckenheimer J, Gueron S (1995a) Dopamine modulation of transient potassium current evokes phase shifts in a central pattern generator network. J Neurosci 15:342-358.
36. Harris-Warrick RM, Coniglio LM, Levini RM, Gueron S, Guckenheimer J (1995b) Dopamine modulation of two subthreshold currents produces phase shifts in activity of an identified motoneuron. J Neurophysiol 74:1404-1420.
37. Wymore RS, Negulescu D, Kinoshita K, Kalman K, Aiyars J, Gutman GA, Chandy KG (1996) Characterization of the transcription unit of mouse Kvl.4, a voltage-gated potassium channel gene. J Biol Chem 271:15629-15634.
38. Xu J, Yu W, Jan YN, Jan LY, Li M (1995) Assembly of voltage-gated potassium channels. J Biol Chem 270:24761-24768.
39. Yu W, Jia X, Li M (1996) NAB domain is essential for the subunit assembly of both α-α and α-β complexes of shaker-like potassium channels. Neuron 16:441-453.
40. + currents in Drosophila muscle by mutations in eag. Science 252:1562-1564.