Assessment of mitochondrial membrane potential in yeast cell populations by flow cytometry

Microbiology

Volumn 147, Issue 12, 2001, Pages 3335-3343

  Ludovico, P ^a   Sansonetty, F ^a   Corte Real M ^a  

^a UNIVERSITY OF MINHO   (Portugal)

Author keywords

Flow cytometry; Mitochondrial functionality; Rhodamine 123; Whole yeast cells

Indexed keywords

DYE; GREEN FLUORESCENT PROTEIN; RHODAMINE 123;

ARTICLE; BINDING AFFINITY; CELL POPULATION; CELL SUSPENSION; CELL VOLUME; CELLULAR DISTRIBUTION; CLINICAL PROTOCOL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CYTOTOXICITY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; HYPERPOLARIZATION; MEMBRANE POTENTIAL; MITOCHONDRIAL ENERGY TRANSFER; MITOCHONDRIAL MEMBRANE; MITOCHONDRIAL RESPIRATION; NONHUMAN; PRIORITY JOURNAL; STAINING; VALIDATION PROCESS; YEAST CELL;

EID: 0035215775     PISSN: 13500872     EISSN: None     Source Type: Journal    
DOI: 10.1099/00221287-147-12-3335     Document Type: Article

References (37)

1. A mitochondrial perspective on cell death
2. The energetics of sugar transport in yeast
3. Mitochondrial membrane potential in living cells
4. Fluorescent labeling of mitochondria
5. Probing mitochondria in living cells with rhodamine 123
6. A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5′,6,6′-tetrachloro-1,1′,3,3′ -tetraethylbenzimidazolcarbocyanine iodide (JC-1)
7. Rhodamine 123 as a probe of transmembrane potential in isolated rat-liver mitochondria: Spectral and metabolic properties
8. Glucose respiration and fermentation in Zygosaccharomyces bailii and Saccharomyces cerevisiae express different sensitivity patterns to ethanol and acetic acid
9. Cell cycle analysis of yeasts using Syber Green I
10. Status of mitochondria in living fibroblasts during growth and senescence in vitro: Use of the laser dye rhodamine 123
11. Conversion of biomembrane-produced energy into electric form. I. Submitochondrial particles
12. Flow cytometric investigation of heterogeneous copper-sensitivity in asynchronously grown Saccharomyces, cerevisiae
13. Localization of mitochondria in living cells with rhodamine 123
14. Monitoring of relative mitochondrial membrane potential in living cells by fluorescence microscopy
15. A fast kinetic method for assessing mitochondrial membrane potential in isolated hepatocytes with rhodamine 123 and flow cytometry
16. Anticancer drugs, ionophoric peptides, and steroids as substrates of the yeast multidrug transporter Pdr5p
17. Misconceptions about the energy metabolism of Saccharomyces cerevisiae
18. Evaluating the mitochondrial (respiratory) function of yeast
19. Flow cytometric monitoring of rhodamine 123 and a cyanine dye uptake by yeast during cider fermentation
20. Saccharomyces cerevisiae commits to a programmed cell death process in response to acetic acid
21. Flow cytometric analysis of mitochondrial activity in situ: Application to acetylceramide-induced mitochondrial swelling and apoptosis
22. Use of rhodamine 123 to investigate alterations in mitochondrial activity in isolated mouse liver mitochondria
23. Analysis of the membrane potential of rat- and mouse-liver mitochondria by flow cytometry and possible applications
24. Discrimination of respiratory dysfunction in yeast mutants by confocal microscopy, image, and flow cytometry
25. Pyruvate metabolism in Saccharomyces cerevisiae
26. Rapid detection of efflux pumps and their relation with drug resistance in yeast cells
27. Mitochondrial analysis in living cells: The use of rhodamine 123 and flow cytometry
28. 6(3) or rhodamine 123, is a reliable fluorescent probe to assess ΔΨ changes in intact cells: Implications for studies on mitochondrial functionality during apoptosis
29. Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: Analysis at the single cell and single organelle level
30. Comparison of indirect probes of membrane potential utilized in studies of human neutrophils
31. Cell membrane potential analysis
32. Flow cytometry as a tool to discriminate respiratory-competent and respiratory-deficient yeast cells
33. Transport-limited fermentation and growth of Saccharomyces cerevisiae and its competitive inhibition
34. Oxygen requirements of fermentative yeasts
35. A rapid characterization of yeast contaminants associated with sparkling wine production