Use of transposon Tn5367 mutagenesis and a nitroimidazopyran-based selection system to demonstrate a requirement for fbiA and fbiB in coenzyme F420 biosynthesis by Mycobacterium bovis BCG

Journal of Bacteriology

Volumn 183, Issue 24, 2001, Pages 7058-7066

  Choi, K P ^a   Bair, T B ^a   Bae, Y M ^a   Daniels, L ^a  

^a UNIVERSITY OF IOWA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; COENZYME F; MESSENGER RNA; NITROIMIDAZOLE DERIVATIVE; PROTEIN FBIA; PROTEIN FBIB; PYRAN DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME SYNTHESIS; GENETIC COMPLEMENTATION; MUTAGENESIS; MYCOBACTERIUM BOVIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS; SEQUENCE HOMOLOGY; STRAIN DIFFERENCE; TRANSPOSON;

ANTITUBERCULAR AGENTS; BACTERIAL PROTEINS; COENZYMES; DNA TRANSPOSABLE ELEMENTS; DRUG RESISTANCE, BACTERIAL; ENZYMES; GENES, BACTERIAL; GENETIC COMPLEMENTATION TEST; MULTIGENE FAMILY; MUTAGENESIS, INSERTIONAL; MYCOBACTERIUM BOVIS; NITROIMIDAZOLES; OPERON; RIBOFLAVIN; SEQUENCE ANALYSIS, DNA; TRANSCRIPTION, GENETIC;

EID: 0035212484     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.24.7058-7066.2001     Document Type: Article

References (47)

1. Basic local alignment search tool
2. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
3. 420 in Mycobacterium species
4. Conditionally replicating mycobacteriophages: A system for transposon delivery to Mycobacterium tuberculosis
5. Complete genome sequence of the methanogenic archaeon Methanococcus jannaschii
6. Rapid amplification of uncharacterized transposon-tagged DNA sequences from genomic DNA
7. Discovery, production, and biological assay of an unusual flavenoid cofactor involved in lincomycin biosynthesis
8. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence
9. Massive gene decay in the leprosy bacillus
10. 420 and properties of its hydrolytic fragments
11. 420 from Methanobacterium
12. Biosynthesis of nucleotides, flavins, and deazaflavins in Methanobacterium thermoautotrophicum
13. Photoreactivating enzyme from the green alga Scenedesmus acutus. Evidence for the presence of two different flavin chromophores
14. DNA photoreactivating enzyme from the cyanobacterium Anacystis nidulans
15. Separation and quantitation of cofactors from methanogenic bacteria by high-performance liquid chromatography: Optimum and routine analysis
16. 420 in the Methanoarchaea
17. 420 dependence of the methylenetetrahydromethanopterin dehydrogenase of Methanobacterium thermoautotrophicum
18. Gene replacement and expression of foreign DNA in mycobacteria
19. 420 biosynthesis in Mycobacterium smegmatis
20. Purification and properties of an 8-hydroxy-5-deazaflavin-reducing hydrogenase from Methanobacterium thermoautotrophicum
21. 420 in methanogenic archaea
22. Reconstitution of a formate-NADP+ oxidoreductase from formate dehydrogenase and a 5-deazaflavin-linked NADP+ reductase isolated from Methanococcus vannielii
23. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions
24. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus
25. 2: Quinone oxidoreductase from Archaeoglobus fulgidus: Characterization of a membrane-bound multisubunit complex containing FAD and iron-sulfur clusters
26. 10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg)
27. An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria
28. Molecular characterization and analysis of the biosynthetic gene cluster for the antitumor antibiotic mitomycin C from Streptomyces lavendulae NRRL 2564
29. In vivo growth characteristics of leucine and methionine auxotrophic mutants of Mycobacterium bovis BCG generated by transposon mutagenesis
30. 420 of Methanobacterium sp
31. Genome sequence of Halobacterium species NRC-1
32. Expression of genes in mycobacteria
33. Efficient allelic exchange and transposon mutagenesis in Mycobacterium tuberculosis
34. 420-dependent glucose-6-phosphate dehydrogenase from Mycobacterium smegmatis
35. 420-dependent glucose-6-phosphate dehydrogenase in Mycobacterium and Nocardia species, but absence in Streptomyces and Corynebacterium species and methanogenic Archaea
36. A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome
37. Biosynthetic precursors of deazaflavins
38. Biochemical and genetic comparison of Streptomyces rimosus mutants impaired in oxytetracyeline biosynthesis
39. Sequence logos: A new way to display consensus sequences
40. Complete genome sequence of Methanobacterium thermoautotrophicum ΔH: Functional analysis and comparative genomics
41. Pattern-induced multisequence alignment (PIMA) algorithm employing secondary structure-dependent gap penalties for use in comparative protein modeling
42. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis
43. A small-molecule nitroimidazopyran drug candidate for the treatment of tuberculosis
44. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
45. 420-specific enzyme from Methanogenium thermophilum strain TCI