Optimization of an oligonucleotide microchip for microbial identification studies: A non-equilibrium dissociation approach

Environmental Microbiology

Volumn 3, Issue 10, 2001, Pages 619-629

  Liu, Wen Tso ^a,c   Mirzabekov, Andrei D ^d   Stahl, David A ^a,b  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b UNIVERSITY OF WASHINGTON   (United States)

^c Northwestern University   (United States)

^d ARGONNE NATIONAL LABORATORY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NASA DISCIPLINE LIFE SCIENCES TECHNOLOGIES; NON-NASA CENTER;

BACILLUS; EVALUATION STUDIES; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; OLIGONUCLEOTIDE PROBES; PHYLOGENY; RNA, BACTERIAL; RNA, RIBOSOMAL; TEMPERATURE;

BACILLI; BACILLUS ANTHRACIS; BACILLUS CEREUS; BACILLUS MYCOIDES; BACILLUS SUBTILIS;

EID: 0035210154     PISSN: 14622912     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1462-2920.2001.00233.x     Document Type: Article

References (31)

1. The oligonucleotide probe database
2. Diversity among Fibrobacter isolates: Towards a phylogenetic classification
3. Phylogenetic identification and in situ detection of individual microbial cells without cultivation
4. Portable system for microbial sample preparation and oligonucleotide microarray analysis
5. Gene organization and primary structure of a ribosomal RNA operon from Esherichia coli
6. The genus Bacillus
7. Optical melting of 128 octamer DNA duplexes
8. Sequence analysis by hybridization with oligonucleotide microchip: Identification of beta-thalassemia mutations
9. Microarrays; their origins and applications
10. Parallel thermodynamic analysis of duplexes on oligodeoxyribonucleotide microchips
11. Flow cytometric analysis of the in situ accessibility of Esherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes
12. Oligonucleotide microchips as genosensors for determinative and environmental studies in microbiology
13. Detection of heterozygous mutations in BRCA1 using high density oligonucleotide arrays and two-colour fluorescent analysis
14. Evolutionary sequence comparisons using high-density oligonucleotide arrays
15. Monitoring the enrichment and isolation of sulfate-reducing bacteria by using oligonucleotide hybridization probes designed from environmentally derived 16S rRNA sequences
16. The RDP (Ribosomal Database Project) continues
17. DNA chips: An array of possibilities
18. Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria
19. A simple method for quantification of uncultured microorganisms in the environment based on in vitro transcription of 16S rRNA
20. Chemical methods of DNA and RNA fluorescent
21. Group-specific 16S rRNA hybridization probes to describe natural communities of methanogens
22. Quantification of methanogenic groups in anaerobic biological reactors using oligonucleotide probe hybridizations
23. Critical evaluation of membrane supports for use in quantitative hybridizations
24. Microchip arrays put DNA on the spot
25. Application of phylogenetically based hybridization probes to microbial ecology
26. Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology
27. Bacterial evolution
28. DNA analysis and diagnostics on oligonucleotide microchips
29. Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies