Tagging Hansenula polymorpha genes by random integration of linear DNA fragments (RALF)

Molecular Genetics and Genomics

Volumn 266, Issue 4, 2001, Pages 646-656

  Van Dijk, R ^a   Faber, K N ^a   Hammond, A T ^b   Glick, B S ^b   Veenhuis, M ^a   Kiel, J A K W ^a  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF CHICAGO   (United States)

Author keywords

Gene tagging; Genomic integration; Mutants; Peroxisome homeostasis; Yeast

Indexed keywords

DNA FRAGMENT; RESTRICTION ENDONUCLEASE;

ARTICLE; BIOGENESIS; CONTROLLED STUDY; DEGRADATION; FUNGAL GENETICS; GENE CASSETTE; GENE FUNCTION; GENE ISOLATION; HANSENULA POLYMORPHA; MUTAGENESIS; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PEROXISOME; PHENOTYPE; PLASMID; PRIORITY JOURNAL; SEQUENCE ANALYSIS;

BASE SEQUENCE; DNA RESTRICTION ENZYMES; GENES, FUNGAL; GENETIC MARKERS; GENOME, FUNGAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PICHIA; PLASMIDS; RECOMBINATION, GENETIC; TRANSFORMATION, GENETIC;

PICHIA ANGUSTA; PROKARYOTA;

EID: 0035207040     PISSN: 16174615     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004380100584     Document Type: Article

References (34)

1. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
2. A stretch of positively charged amino acids at the N terminus of Hansenula polymorpha Pex3p is involved in incorporation of the protein into the peroxisomal membrane
3. Genomic exploration of the hemiascomycetous yeasts. 13. Pichia angusta
4. Tagging pathogenicity genes in Ustilago maydis by restriction enzyme-mediated integration (REMI)
5. Stable transformation and regulated expression of an inducible reporter construct in Candida albicans using restriction enzyme-mediated integration
6. Insertional mutagenesis of Aspergillus fumigatus
7. Peroxisome-deficient mutants of Hansenula polymorpha
8. Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance
9. Chromosomal targeting of replicating plasmids in the yeast Hansenula polymorpha
10. The methylotrophic yeast Hansenula polymorpha contains an inducible import pathway for peroxisomal matrix proteins with an N-terminal targeting signal (PTS2 proteins)
11. Highly-efficient electrotransformation of the yeast Hansenula polymorpha
12. Bleomycin resistance conferred by a drug-binding protein
13. Genetic analysis in the methylotrophic yeast Hansenula polymorpha
14. Restriction enzyme-mediated DNA integration in Coprinus cinereus
15. Characterization of a novel component of the peroxisomal protein import apparatus using fluorescent peroxisomal proteins
16. LbrA, a protein predicted to have a role in vesicle trafficking, is necessary for normal morphogenesis in Polysphondylium pallidura
17. Hansenula polymorpha Pex1p and Pex6p are peroxisome-associated AAA proteins that functionally and physically interact
18. Tagging developmental genes in Dictyostelium by restriction enzyme-mediated integration of plasmid DNA
19. Deletions in the gibberellin biosynthesis gene cluster of Gibberella fujikuroi by restriction enzyme-mediated integration and conventional transformation-mediated mutagenesis
20. Insertion of in-frame sequence tags into proteins using transposons
21. Overproduction of Pex5p stimulates import of alcohol oxidase and dihydroxyacetone synthase in a Hansenula polymorpha pex14 null mutant
22. Increased transformation frequency and tagging of developmental genes in Aspergillus nidulans by restriction enzyme-mediated integration (REMI)
23. Transformation of the edible basidiomycete Lentinus edodes by restriction enzyme-mediated integration of plasmid DNA
24. Integration of DNA fragments by illegitimate recombination in Saccharomyces cerevisiae
25. Promoter-tagged restriction enzyme-mediated insertion (PT-REMI) mutagenesis in Aspergillus niger
26. The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation
27. Isolation and characterization of mutants impaired in the selective degradation of peroxisomes in the yeast Hansenula polymorpha
28. Methanol metabolism in a peroxisome-deficient mutant of Hansenula polymorpha: A physiological study
29. The methylotrophic yeast Hansenula polymorpha: A versatile cell factory
30. Growth of Hansenula polymorpha in a methanol-limited chemostat. Physiological responses due to the involvement of methanol oxidase as a key enzyme in methanol metabolism
31. Degradation and turnover of peroxisomes in the yeast Hansenula polymorpha induced by selective inactivation of peroxisomal enzymes
32. Peroxisome biogenesis and degradation in yeast: A structure/function analysis
33. Isolation and characterization of peroxisomal protein import (pim-) mutants of Hansenula polymorpha