Rapid identification of Bordetella pertussis pertactin gene variants using lightcycler real-time polymerase chain reaction combined with melting curve analysis and gel electrophoresis

Emerging Infectious Diseases

Volumn 7, Issue 6, 2001, Pages 952-958

  Mäkinen, Johanna ^a,b   Viljanen, Matti K ^a   Mertsola, Jussi ^b   Arvilommi, Heikki ^a   He, Qiushui ^a  

^a NATIONAL PUBLIC HEALTH INSTITUTE   (Finland)

^b TURKU UNIVERSITY HOSPITAL   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PERTUSSIS VACCINE; PROTEIN PRN; UNCLASSIFIED DRUG;

ALLELE; ARTICLE; ASSAY; BACTERIAL GENE; BACTERIUM ISOLATION; BORDETELLA PERTUSSIS; CONTROLLED STUDY; EUROPE; GEL ELECTROPHORESIS; GENE SEQUENCE; GENETIC VARIABILITY; METHODOLOGY; NONHUMAN; POLYMERASE CHAIN REACTION; RELIABILITY; SCREENING; STRAIN DIFFERENCE;

EID: 0035194019     PISSN: 10806040     EISSN: None     Source Type: Journal    
DOI: 10.3201/eid0706.010606     Document Type: Article

References (26)

1. Pertussis notifications in Australia
2. Return of epidemic pertussis in the United States
3. The return of pertussis
4. Pertussis in the Netherlands: An outbreak despite high levels of immunization with whole cell vaccine
5. Pertussis in Quebec: Ongoing epidemic since the late 1980s
6. Identification of a 69-kilodalton nonfimbrial protein as an agglutinogen of Bordetella pertussis
7. A search for serologic correlates of immunity to Bordetella pertussis cough illness
8. Characterization of the protective capacity and immunogenicity of the 69-kD outer membrane protein of Bordetella pertussis
9. Levels of anti-pertussis antibodies related to protection after household exposure to Bordetella pertussis
10. Molecular cloning and characterization of protective outer membrane protein P.69 from Bordetella pertussis
11. Identification and characterization of a protective immunodominant B cell epitope of pertactin (P.69) from Bordetella pertussis
12. Polymorphism in the Bordetella pertussis virulence factors P.69/pertactin and pertussis toxin in the Netherlands: Temporal trends and evidence for vaccine-driven evolution
13. Epidemiological typing of Bordetella pertussis isolates: Recommendations for a standard methodology
14. Polymorphism in Bordetella pertussis pertactin and pertussis toxin virulence factors in the United States, 1935-1999
15. Variation in the Bordetella pertussis virulence factors pertussis toxin and pertactin in vaccine strains and clinical isolates in Finland
16. Antigenic variants in Bordetella pertussis strains isolated from vaccinated and unvaccinated children
17. Application of a thermodynamic nearest-neighbour model to estimate nucleic acid stability and optimize probe design: Prediction of melting points of multiple mutations of apolipoprotein B-3500 and factor V with a hybridization probe genotyping assay on the Light Cycler
18. Fluorescence melting curve analysis for the detection of the bcl-1/ JH translocation in mantle cell lymphoma
19. Different real-time PCR formats compared for the quantitative detection of human cytomegalovirus DNA
20. The LightCycler: A microvolume multisample fluorimeter with rapid temperature control
21. Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferii sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene
22. Continuous fluorescence monitoring of rapid cycle DNA amplification
23. Comparison of polymerase chain reaction with culture and enzyme immunoassay for diagnosis of pertussis
24. Allele-specific PCR method based on pncA and oxyR sequences for distinguishing Mycobacterium bovis from M. tuberculosis: Intraspecific M. bovis pncA sequence polymorphism
25. Simultaneous identification of strains of Escherichia coli serotype O157:H7 and their Shigalike toxin type by mismatch amplification mutation assay-multiplex PCR
26. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction