System for cleavable Fc fusion proteins using tobacco etch virus (TEV) protease

BioTechniques

Volumn 30, Issue 1, 2001, Pages 60-66

  Haspel, J ^a   Blanco, C ^a   Jacob, J ^a   Grumet, M ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL ADHESION MOLECULE; HYBRID PROTEIN; IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G; PROTEINASE;

ARTICLE; EMBRYO; ENZYME ACTIVITY; ENZYME SPECIFICITY; HUMAN; HUMAN CELL; NERVE FIBER GROWTH; NONHUMAN; PLANT VIRUS; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN DOMAIN;

NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 0035191129     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01301st01     Document Type: Article

References (12)

1. Generation of soluble interleukin-1 receptor from an immunoadhesin by specific cleavage
2. Immunoadhesins: Principles and applications
3. Expression and characterization of a DNase I-Fc fusion enzyme
4. Critical and optimal Ig domains for promotion of neurite outgrowth by L1/Ng-CAM
5. Cleavable CD40Ig fusion proteins and the binding to sgp39
6. Immunoadhesins of interleukin-6 and the IL-6/soluble IL-6R fusion protein hyper-IL-6
7. A modular set of prokaryotic and eukaryotic expression vectors
8. Release of proteins and peptides from fusion proteins using a recombinant plant virus protease
9. TEV protease, recombinant: A site-specific protease for efficient cleavage of affinity tags from expressed proteins
10. New baculovirus expression vectors for the purification of recombinant protein from insect cells
11. Purification of Ig-fusion proteins from medium containing Ig
12. Calcium influx into neurons can solely account for cell contact-dependent neurite outgrowth stimulated by transfected L1