Cost-efficient quantification of enzyme-linked immunospot

BioTechniques

Volumn 30, Issue 1, 2001, Pages 36-38

  Bharadwaj, M ^a   Parsons, P G ^a   Moss, D J ^a  

^a QIMR BERGHOFER MEDICAL RESEARCH INSTITUTE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOKINE;

ANALYTIC METHOD; ARTICLE; COST EFFECTIVENESS ANALYSIS; CYTOKINE RELEASE; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNE RESPONSE; IMMUNOSTIMULATION; T LYMPHOCYTE ACTIVATION;

EID: 0035185412     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/01301bm05     Document Type: Article

References (9)

1. Computer-assisted, quantitative cytokine enzyme-linked immunospot analysis of human immune effector cell function
2. Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells
3. Immunogenicities of intravenous and intramuscular administrations of modified vaccinia virus Ankara-based multi-CTL epitope vaccine for human immunodeficiency virus type 1 in mice
4. The use of computer-assisted video image analysis for the quantification of CD8+ T lymphocytes producing tumor necrosis factor alpha spots in response to peptide antigens
5. A sensitive ELISPOT assay to detect low-frequency human T lymphocytes
6. A sensitive ELISPOT assay for detection of CD8+ T lymphocytes specific for HLA class I-binding peptide epitopes derived from influenza proreins in the blood of healthy donors and melanoma patients
7. Evaluation of the interferon-gamma ELISPOT-assay for quantification of peptide specific T lymphocytes from peripheral blood
8. Digital quantification of the enzyme-linked immunospot (ELISPOT)
9. Application of ELISPOT assay to the characterization of CD8+ responses to Epstein-Barr virus antigens