The use of computer-assisted video image analysis for the quantification of CD8+T lymphocytes producing tumor necrosis factor α spots in response to peptide antigens

Journal of Immunological Methods

Volumn 203, Issue 2, 1997, Pages 141-152

  Herr, Wolfgang ^a   Linn, Bruno ^b   Leister, Nicole ^a   Wandel, Eveline ^a   Meyer Zum Büschenfelde, Karl Hermann ^a   Wölfel, Thomas ^a  

^a JOHANNES GUTENBERG UNIVERSITY   (Germany)

^b Leica GmbH   (Germany)

Author keywords

CD8+ T lymphocytes; CMV; ELISPOT assay; image analysis; influenza; peptides; TNF

Indexed keywords

CD8 ANTIGEN; HLA A2 ANTIGEN; SYNTHETIC PEPTIDE; TUMOR NECROSIS FACTOR ALPHA;

ADULT; ARTICLE; CASE REPORT; CONTROLLED STUDY; CYTOMEGALOVIRUS INFECTION; ENZYME IMMUNOASSAY; HUMAN; HUMAN CELL; IMAGE ANALYSIS; PRIORITY JOURNAL; T LYMPHOCYTE SUBPOPULATION;

EID: 0030934741     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0022-1759(97)00019-7     Document Type: Article

References (39)

1. Bednarek M.A., Sauma S.Y., Gammon M.C., Porter G., Tamhankar S., Williamson A.R., Zweerink H.J. The minimum peptide epitope from the influenza virus matrix protein. Extra and intracellular loading of HLA-A2. J. Immunol. 147:1991;4047.
2. Bessis, M. (1973) Lymphocytic series. II. Description of lymphocytic cells. In: Living blood cells and their ultrastructure. Springer-Verlag, Berlin, p. 419.
3. Boon T., Gajewski T.F., Coulie P.G. From defined human tumor antigens to effective immunization. Immunol. Today. 16:1995;334.
4. Borysiewicz L.K., Hickling J.K., Graham S., Sinclair J., Cranage M.P., Smith G.L., Sissons J.G.P. Human cytomegalovirus-specific cytotoxic T cells: Relative frequency of stage-specific CTL recognizing the 72-kD immediate early protein and glycoprotein B expressed by recombinant vaccinia viruses. J. Exp. Med. 168:1988;919.
5. Borysiewicz L.K., Fiander A., Nimako M., Man S., Wilkinson G.W.G., Westmoreland D., Evans A.S., Adams M., Stacey S.N., Boursnell M.E.G., Rutherford E., Hickling J.K., Inglis S.C. A recombinant vaccinia virus encoding human papillomavirus types 16 and 18, E6 and E7 proteins as immunotherapy for cervical cancer. Lancet. 347:1996;1523.
6. Brichard V., Van Pel A., Wölfel T., Wölfel C., De Plaen E., Lethé B., Coulie P., Boon T. The tyrosinase gene codes for an antigen recognized by autologous cytolytic T lymphocytes on HLA-A2 melanomas. Eur. J. Immunol. 178:1993;489.
7. Carmichael A., Jin X., Sissons P., Boryieswicz L. Quantitative analysis of the human immunodeficiency virus type 1 (HIV-1)-specific cytotoxic T lymphocyte (CTL) response at different stages of HIV-1 infection: Different CTL responses to HIV-1 and Epstein-Barr Virus in late disease. J. Exp. Med. 177:1993;249.
8. Cerundolo V., Alexander J., Anderson K., Lamb C., Cresswell P., McMichael A., Gotch F., Townsend A. Presentation of viral antigen controlled by a gene in the major histocompatibility complex. Nature. 345:1990;449.
9. Cohen J. Vaccines get a new twist. Science. 264:1994;503.
10. Coulie P., Somville M., Lehmann F., Hainaut P., Brasseur F., Devos R., Boon T. Precursor frequency analysis of human cytolytic T lymphocytes directed against autologous melanoma cells. Int. J. Cancer. 50:1992;289.
11. Czerkinsky C., Andersson G., Ekre H.-P., Nilsson L.-A., Klareskog L., Ouchterlony Ö. Reverse ELISPOT assay for clonal analysis of cytokine production. I Enumeration of gamma-interferon-secreting cells. J. Immunol. Methods. 110:1988;29.
12. Herr W., Wölfel T., Heike M., Meyer zum Büschefelde K.-H., Knuth A. Frequency analysis of tumor-reactive cytotoxic T lymphocytes in peripheral blood of a melanoma patient vaccinated with autologous tumor cells. Cancer Immunol. Immunother. 39:1994;93.
13. + T lymphocytes secreting tumor necrosis factor α in response to HLA-A2.1-binding melanoma and viral peptide antigens. J. Immunol. Methods. 191:1996;131.
14. Herzog W.R., Zanker B., Irschick E., Huber C., Franz H.E., Wagner H., Kabelitz D. Selective reduction of donor-specific cytotoxic T lymphocyte precursors in patients with a well-functioning kidney allograft. Transplantation. 43:1987;384.
15. Jäger E., Bernhard H., Romero P., Ringhoffer M., Arand M., Karbach J., Ilsemann C., Hagedorn M., Knuth A. Generation of cytotoxic T-cell responses with synthetic melanoma-associated peptides in vivo: Implications for tumor vaccines with melanoma-associated antigens. Int. J. Cancer. 66:1996;162.
16. Jarvis L.R. Microcomputer video image analysis. J. Microsc. 150:1988;83.
17. Kabilan L., Andersson G., Lolli F., Ekre H.-P., Olsson T., Troye-Blomberg M. Detection of intracellular expression and secretion of interferon-γ at the single-cell level after activation of human T cells with tetanus toxoid in vitro. Eur. J. Immunol. 20:1990;1085.
18. Kohlberger P.D., Obermair A., Sliutz G., Heinzl H., Koelbl H., Breitenecker G., Gitsch G., Kainz C. Quantitative immunohistochemistry of Factor VIII-related antigen in breast carcinoma. A comparison of computer-assisted image analysis with established counting methods. Am. J. Clin. Pathol. 105:1996;705.
19. Lewis C.L., McCracken D., Ling R., Richards P.S., McCarthy S.P., McGee J.O.D. Cytokine release by single, immunophenotyped human cells: Use of the reverse hemolytic plaque assay. Immunol. Rev. 119:1991;23.
20. Mahanty S., Abrams J.S., King C.L., Limaye A.P., Nutman T.B. Parallel regulation of IL-4 and IL-5 in human helminth infections. J. Immunol. 148:1992;3567.
21. Marchand M., Weynants P., Rankin E., Arienti F., Belli F., Parmiani G., Cascinelli N., Bourlond A., Vanwijck R., Humblet Y., Canon J.-L., Laurent C., Naeyaert J.-M., Plagne R., Deraemaeker R., Knuth A., Jäger E., Brasseur F., Herman J., Coulie P.G., Boon T. Tumor regression responses in melanoma patients treated with a peptide encoded by gene MAGE-3. Int. J. Cancer. 63:1995;883.
22. McMichael A.J., Gotch F.M., Noble G.R., Beare P.A.S. Cytotoxic T-cell immunity to influenza. N. Engl. J. Med. 309:1983;13.
23. + T cells using an ELISPOT assay. J. Immunol. Methods. 181:1995;45.
24. Nanda N.K., Sercarz E.E. Induction of anti-self-immunity to cure cancer. Cell. 82:1995;13.
25. Norazmi M.-N., Hohmann A.W., Jarvis L.R., Skinner J.M., Stoll P., Bradley J. The use of computer-assisted video image analysis in the enumeration of immuno-stained cells in tissue sections. J. Immunol. Methods. 131:1990;223.
26. Riddell S.R., Watanabe K.S., Goodrich J.M., Li C.R., Agha M.E., Greenberg P.D. Restoration of viral immunity in immunodeficient humans by the adoptive transfer of T cell clones. Science. 257:1992;238.
27. Rodrigues M.M., Li S., Murata K., Rodriguez D., Rodriguez J.R., Bacik I., Bennink J., Yewdell J., Garcia-Sastre A., Esteban M., Palese P., Nussenzweig R.S., Zavala F. Influenza and vaccinia viruses expressing malaria T and B cell epitopes in different structural arrangements: Comparison of their immunogenicity and capacity to induce protective immunity. J. Immunol. 153:1994;4636.
28. Salter R.D., Howell D.N., Cresswell P. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics. 21:1985;235.
29. Sharrock C.E.M., Kaminski E., Man S. Limiting dilution analysis of human T cells: a useful clinical tool. Immunol. Today. 11:1990;281.
30. Skipper J.C.A., Hendrickson R.C., Gulden P.H., Brichard V., Van Pel A., Chen Y., Shabanowitz J., Wölfel T., Slingluff G.L. Jr., Boon T., Hunt D.F., Engelhard V.H. An HLA-A2-restricted tyrosinase antigen on melanoma cells resulted from posttranslational modification and suggests a novel pathway for processing of membrane proteins. J. Exp. Med. 183:1996;527.
31. Tsang K.Y., Zaremba S., Nieroda C.A., Zhu M.Z., Hamilton J.M., Schlom J. Generation of human cytotoxic T cells specific for human carcinoembryonic antigen epitopes from patients immunized with recombinant vaccinia-CEA vaccine. J. Natl. Cancer Inst. 87:1995;982.
32. + T cells binds very tightly to the restricting class I major histocompatibility complex protein on intact cells but not to the purified class I protein. Proc. Natl. Acad. Sci. USA. 88:1991;11276.
33. + αβ T cell and γδ T cell responses to Mycobacterium tuberculosis. J. Immunol. 154:1995;1786.
34. Utz U., Koenig S., Coligan J.E., Biddison W.E. Presentation of three different viral peptides, HTLV-1 Tax, HCMV gB, and influenza virus M1, is determined by common structural features of the HLA-A2.1 molecule. J. Immunol. 149:1992;214.
35. Visseren M.J.W., Van Elsas A., Van der Voort E.I.H., Ressing M.E., Kast W.M., Schrier P.I., Melief C.J.M. CTL specific for tyrosinase autoantigen can be induced from healthy donor blood to lyse melanoma cells. J. Immunol. 154:1995;3991.
36. Vitiello A., Ishioka G., Grey H.M., Rose R., Farness P., LaFond R., Yuan L., Chisari F.V., Furze J., Bartholomeuz R., Chesnut R.W. Development of a lipopeptide-based therapeutic vaccine to treat chronic HBV infection. I. Induction of a primary cytotoxic T lymphocyte response in humans. J. Clin. Invest. 95:1995;341.
37. Wölfel T., Van Pel A., Brichard V., Schneider J., Seliger B., Meyer zum Büschenfelde K.-H., Boon T. Two tyrosinase nonapeptides recognized on HLA-A2 melanomas by autologous cytolytic T lymphocytes. Eur. J. Immunol. 24:1994;759.
38. Wucherpfennig K.W., Strominger J.L. Molecular mimicry in T cell-mediated autoimmunity: Viral peptides activate human T cell clones specific for myelin basic protein. Cell. 80:1995;695.
39. Yi Q., Lefvert A.K. Idiotypic and antiidiotypic T and B lymphocytes in myasthenia gravis. J. Immunol. 149:1992;3423.